{"product_id":"tris-glycine-sds-running-buffer-powder","title":"Tris-Glycine-SDS Electrophoresis Buffer Instant Powder","description":"\u003c!-- =========================\nPRODUCT DESCRIPTION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eDuring routine SDS-PAGE protein electrophoresis workflows, preparing running buffer manually can introduce variability caused by repeated weighing, reagent handling, and preparation errors. Tris-Glycine-SDS Electrophoresis Buffer Instant Powder provides a convenient solution for researchers who need a consistent SDS-PAGE running buffer preparation workflow with reduced preparation steps.\u003c\/p\u003e\n\u003cp\u003eTris-Glycine-SDS Electrophoresis Buffer Instant Powder is a pre-measured powder formulation designed for preparation of Tris-Glycine-SDS running buffer used in denaturing polyacrylamide gel electrophoresis. Each package can be dissolved and brought to a final volume of 1 L to prepare SDS-PAGE electrophoresis buffer for protein separation applications.\u003c\/p\u003e\n\u003cp\u003eThis SDS-PAGE running buffer contains SDS and is suitable for Tris-HCl based polyacrylamide gel electrophoresis systems. It is commonly used for denaturing protein electrophoresis workflows, including protein separation prior to downstream analysis such as Western blotting. This product is not suitable for polyacrylamide gel systems using alternative buffer systems such as HEPES.\u003c\/p\u003e\n\u003cp\u003eThe instant powder format provides convenient room temperature storage and efficient laboratory preparation compared with manually prepared electrophoresis buffers.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nSPECIFICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eTris-Glycine-SDS Electrophoresis Buffer Instant Powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eAP0541\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eSDS-PAGE Running Buffer Powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eInstant Powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSpecification\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e10 × 1 L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eSDS-PAGE, protein electrophoresis, Western blot workflows\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eCompatible Gel System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eTris-HCl based polyacrylamide gels\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eRoom Temperature\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e2 years\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFEATURES\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eConvenient powder format:\u003c\/strong\u003e Pre-measured instant powder simplifies SDS-PAGE running buffer preparation and reduces manual reagent handling.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eDesigned for SDS-PAGE protein electrophoresis:\u003c\/strong\u003e Contains SDS and supports denaturing protein separation using Tris-Glycine buffer systems.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eConsistent buffer preparation:\u003c\/strong\u003e Each package is prepared to a final volume of 1 L for convenient laboratory use.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRoom temperature storage:\u003c\/strong\u003e Powder format enables convenient storage with a 2-year shelf life under recommended conditions.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCompatible with downstream protein analysis:\u003c\/strong\u003e Suitable for protein separation workflows before applications such as Western blot analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eReusable running buffer option:\u003c\/strong\u003e Prepared electrophoresis buffer may be recovered and reused as an outer chamber running buffer for limited cycles.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nAPPLICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eTris-Glycine-SDS Electrophoresis Buffer Instant Powder is designed for protein electrophoresis workflows requiring reliable SDS-PAGE running buffer preparation.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSDS-PAGE Protein Electrophoresis:\u003c\/strong\u003e Used as a running buffer for denaturing polyacrylamide gel electrophoresis to separate proteins according to molecular characteristics.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eWestern Blot Sample Preparation:\u003c\/strong\u003e Supports protein separation workflows before transfer and antibody-based protein detection.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein Molecular Analysis:\u003c\/strong\u003e Provides electrophoresis conditions for routine protein characterization and comparison studies.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Protein Research:\u003c\/strong\u003e Suitable for analyzing protein expression profiles during molecular biology research workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCell Biology Research:\u003c\/strong\u003e Used with protein lysates prepared from biological samples for SDS-PAGE analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBiochemical Research:\u003c\/strong\u003e Supports common protein separation workflows requiring Tris-Glycine-SDS electrophoresis systems.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPROTOCOL OVERVIEW\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eProtocol Overview\u003c\/h2\u003e\n\u003cp\u003eOne package of Tris-Glycine-SDS Electrophoresis Buffer Instant Powder is used to prepare 1 L SDS-PAGE running buffer.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eAdd one package of powder into a clean container.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdd approximately 900 mL distilled water and dissolve completely.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdjust the final volume to 1 L with distilled water.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMix thoroughly before use in SDS-PAGE electrophoresis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eUnused prepared buffer can typically be stored at room temperature for 2–3 days or at 4°C for 1–2 weeks. For optimal electrophoresis performance, freshly prepared running buffer is recommended.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nORDERING INFORMATION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eOrdering Information\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eAP0541\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eTris-Glycine-SDS Electrophoresis Buffer Instant Powder\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e10 × 1 L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFAQ\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is Tris-Glycine-SDS Electrophoresis Buffer used for?\u003c\/summary\u003e\n\u003cp\u003eTris-Glycine-SDS Electrophoresis Buffer is a running buffer used for SDS-PAGE protein electrophoresis. It supports denaturing protein separation in Tris-HCl based polyacrylamide gel systems.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the difference between Tris-Glycine-SDS buffer and native PAGE buffer?\u003c\/summary\u003e\n\u003cp\u003eTris-Glycine-SDS running buffer contains SDS and is designed for denaturing protein electrophoresis. Native PAGE workflows typically require different buffer systems without SDS.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan this SDS-PAGE running buffer be used for Western blot workflows?\u003c\/summary\u003e\n\u003cp\u003eYes. This Tris-Glycine-SDS running buffer can be used during protein separation before downstream Western blot analysis.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow do I prepare the instant powder electrophoresis buffer?\u003c\/summary\u003e\n\u003cp\u003eDissolve one package of powder in distilled water, initially using approximately 900 mL water, then adjust the final volume to 1 L before use.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan prepared running buffer be reused?\u003c\/summary\u003e\n\u003cp\u003ePrepared electrophoresis buffer may be recovered as an outer chamber running buffer and reused 1–2 times. For optimal SDS-PAGE performance, fresh running buffer is recommended.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs this buffer compatible with HEPES-based PAGE systems?\u003c\/summary\u003e\n\u003cp\u003eNo. This product is designed for Tris-HCl based polyacrylamide gel electrophoresis systems and is not recommended for HEPES-based PAGE systems.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPRODUCT SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!-- =========================\nFAQ SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AccuRef Scientific","offers":[{"title":"10×1L","offer_id":52784047849653,"sku":"AP0541","price":42.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_2edf4177-51c1-477a-8a3d-bc7106f4eca4.jpg?v=1783669335","url":"https:\/\/biofargo.com\/products\/tris-glycine-sds-running-buffer-powder","provider":"Biofargo","version":"1.0","type":"link"}