Description

Tris-Glycine-SDS Electrophoresis Buffer (10×) is a concentrated SDS-PAGE running buffer designed for reliable protein separation using the traditional Tris-Glycine gel electrophoresis system. During protein electrophoresis workflows, unstable buffer conditions, incorrect ionic balance, or unsuitable running solutions can affect protein migration, band resolution, and downstream Western blot analysis. This 10X Tris Glycine SDS Running Buffer provides a convenient solution for preparing consistent electrophoresis conditions for denaturing protein separation.

Formulated with the classic Tris-Glycine-SDS buffer system, this protein electrophoresis buffer supports SDS-mediated protein denaturation and size-based separation on Tris-HCl polyacrylamide gels. The prepared 1X working solution is suitable for SDS-PAGE gel electrophoresis workflows, including recombinant protein analysis, cell lysate evaluation, and Western blot sample preparation.

Tris-Glycine-SDS Electrophoresis Buffer (10×) is supplied as a concentrated formulation that can be diluted with deionized water before use, reducing storage requirements and improving laboratory workflow efficiency. This running buffer is compatible with standard Tris-Glycine SDS-PAGE systems and is not intended for electrophoresis systems based on alternative buffer chemistries such as HEPES gel systems.

Specifications

Product Name	Tris-Glycine-SDS Electrophoresis Buffer (10×)
SKU	AP0281
Volume	500 mL
Product Type	10X Tris Glycine SDS Running Buffer
Buffer System	Tris-Glycine-SDS
Working Concentration	1X after 10-fold dilution
Compatible Gel System	Tris-HCl polyacrylamide gels
Applications	SDS-PAGE, protein electrophoresis, Western blot workflows
Storage	Room temperature
Shelf Life	18 months

Features

Classic SDS-PAGE running buffer formulation: Uses the widely adopted Tris-Glycine-SDS system for denaturing protein electrophoresis.
10X concentrated format: Provides a space-efficient electrophoresis buffer solution that can be easily diluted to prepare 1X working buffer.
Reliable protein separation: Supports consistent protein migration and molecular weight-based separation during SDS-PAGE.
Compatible with Western blot workflows: Suitable for protein electrophoresis steps before membrane transfer and antibody detection.
Convenient laboratory preparation: Reduces preparation time compared with manually preparing individual Tris, glycine, and SDS components.
Reusable electrophoresis buffer option: Used running buffer may be recovered for external chamber electrophoresis applications when appropriate.

Applications

Tris-Glycine-SDS Electrophoresis Buffer (10×) is designed for protein electrophoresis applications in molecular biology, biochemistry, and life science research laboratories.

SDS-PAGE Protein Separation: Provides the running environment required for denaturing polyacrylamide gel electrophoresis and protein molecular weight analysis.
Western Blot Sample Analysis: Supports electrophoretic separation of proteins before transfer to PVDF or nitrocellulose membranes.
Recombinant Protein Expression Analysis: Used for evaluating recombinant protein production and purification results through SDS-PAGE.
Cell Lysate Protein Analysis: Suitable for separation and analysis of proteins extracted from cultured cells.
Tissue Protein Electrophoresis: Supports protein profiling from tissue-derived samples in biochemical research workflows.
Protein Molecular Weight Determination: Enables comparison of protein migration patterns with molecular weight markers.
Protein Purification Evaluation: Used to assess purification fractions and protein sample quality after chromatography workflows.
General Biochemical Research: Suitable for routine protein electrophoresis experiments requiring a standard Tris-Glycine SDS buffer system.

Protocol Overview

Prepare the working electrophoresis buffer before SDS-PAGE experiments according to laboratory requirements.

Dilute 10X Tris-Glycine-SDS Electrophoresis Buffer with deionized water at a 1:9 ratio to prepare 1X running buffer.
Mix thoroughly before adding the buffer to the electrophoresis chamber.
Load prepared protein samples onto Tris-HCl polyacrylamide gels and perform SDS-PAGE according to the selected electrophoresis conditions.
After electrophoresis, proteins can be visualized by staining or transferred for Western blot detection.

If precipitation occurs after low-temperature storage or transportation, allow the solution to return to room temperature and mix until fully dissolved before use.

Ordering Information

SKU	Product	Size
AP0281	Tris-Glycine-SDS Electrophoresis Buffer (10×)	500 mL

FAQ

What is Tris-Glycine-SDS Electrophoresis Buffer used for?

Tris-Glycine-SDS Electrophoresis Buffer is a protein running buffer used for SDS-PAGE electrophoresis. It provides the ionic environment required for separating denatured proteins according to molecular weight in Tris-Glycine gel systems.

How do you prepare 1X SDS-PAGE running buffer from 10X Tris Glycine SDS Buffer?

Prepare 1X running buffer by diluting one part of 10X Tris-Glycine-SDS Electrophoresis Buffer with nine parts of deionized water. For example, mix 100 mL of 10X buffer with 900 mL water to prepare 1 L of working solution.

Can this Tris Glycine SDS Running Buffer be used for Western blot?

Yes. This SDS-PAGE running buffer is commonly used for protein separation before Western blot transfer and detection workflows.

What type of gels are compatible with this electrophoresis buffer?

This buffer is designed for Tris-HCl polyacrylamide gel systems using the standard Tris-Glycine-SDS electrophoresis method. It is not recommended for HEPES-based gel systems or alternative buffer chemistries.

Can used SDS-PAGE running buffer be reused?

Recovered running buffer may be reused for external chamber electrophoresis applications 1–2 times. However, fresh running buffer is recommended when maximum electrophoresis performance and reproducibility are required.

Why does the buffer sometimes show precipitation?

Precipitation may occur after cold storage or transportation because some components can crystallize at low temperatures. Allowing the solution to warm to room temperature and mixing for approximately 10–30 minutes usually restores the solution.