{"product_id":"tris-glycine-sds-running-buffer-10x","title":"Tris-Glycine-SDS Electrophoresis Buffer (10×)","description":"\u003c!-- =========================\nPRODUCT DESCRIPTION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eTris-Glycine-SDS Electrophoresis Buffer (10×) is a concentrated SDS-PAGE running buffer designed for reliable protein separation using the traditional Tris-Glycine gel electrophoresis system. During protein electrophoresis workflows, unstable buffer conditions, incorrect ionic balance, or unsuitable running solutions can affect protein migration, band resolution, and downstream Western blot analysis. This 10X Tris Glycine SDS Running Buffer provides a convenient solution for preparing consistent electrophoresis conditions for denaturing protein separation.\u003c\/p\u003e\n\u003cp\u003eFormulated with the classic Tris-Glycine-SDS buffer system, this protein electrophoresis buffer supports SDS-mediated protein denaturation and size-based separation on Tris-HCl polyacrylamide gels. The prepared 1X working solution is suitable for SDS-PAGE gel electrophoresis workflows, including recombinant protein analysis, cell lysate evaluation, and Western blot sample preparation.\u003c\/p\u003e\n\u003cp\u003eTris-Glycine-SDS Electrophoresis Buffer (10×) is supplied as a concentrated formulation that can be diluted with deionized water before use, reducing storage requirements and improving laboratory workflow efficiency. This running buffer is compatible with standard Tris-Glycine SDS-PAGE systems and is not intended for electrophoresis systems based on alternative buffer chemistries such as HEPES gel systems.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nSPECIFICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eTris-Glycine-SDS Electrophoresis Buffer (10×)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eAP0281\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eVolume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e10X Tris Glycine SDS Running Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eBuffer System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eTris-Glycine-SDS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eWorking Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e1X after 10-fold dilution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eCompatible Gel System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eTris-HCl polyacrylamide gels\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eSDS-PAGE, protein electrophoresis, Western blot workflows\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eRoom temperature\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e18 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFEATURES\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eClassic SDS-PAGE running buffer formulation:\u003c\/strong\u003e Uses the widely adopted Tris-Glycine-SDS system for denaturing protein electrophoresis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e10X concentrated format:\u003c\/strong\u003e Provides a space-efficient electrophoresis buffer solution that can be easily diluted to prepare 1X working buffer.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eReliable protein separation:\u003c\/strong\u003e Supports consistent protein migration and molecular weight-based separation during SDS-PAGE.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCompatible with Western blot workflows:\u003c\/strong\u003e Suitable for protein electrophoresis steps before membrane transfer and antibody detection.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eConvenient laboratory preparation:\u003c\/strong\u003e Reduces preparation time compared with manually preparing individual Tris, glycine, and SDS components.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eReusable electrophoresis buffer option:\u003c\/strong\u003e Used running buffer may be recovered for external chamber electrophoresis applications when appropriate.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nAPPLICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eTris-Glycine-SDS Electrophoresis Buffer (10×) is designed for protein electrophoresis applications in molecular biology, biochemistry, and life science research laboratories.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSDS-PAGE Protein Separation:\u003c\/strong\u003e Provides the running environment required for denaturing polyacrylamide gel electrophoresis and protein molecular weight analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eWestern Blot Sample Analysis:\u003c\/strong\u003e Supports electrophoretic separation of proteins before transfer to PVDF or nitrocellulose membranes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Protein Expression Analysis:\u003c\/strong\u003e Used for evaluating recombinant protein production and purification results through SDS-PAGE.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCell Lysate Protein Analysis:\u003c\/strong\u003e Suitable for separation and analysis of proteins extracted from cultured cells.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTissue Protein Electrophoresis:\u003c\/strong\u003e Supports protein profiling from tissue-derived samples in biochemical research workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein Molecular Weight Determination:\u003c\/strong\u003e Enables comparison of protein migration patterns with molecular weight markers.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein Purification Evaluation:\u003c\/strong\u003e Used to assess purification fractions and protein sample quality after chromatography workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eGeneral Biochemical Research:\u003c\/strong\u003e Suitable for routine protein electrophoresis experiments requiring a standard Tris-Glycine SDS buffer system.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPROTOCOL OVERVIEW\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eProtocol Overview\u003c\/h2\u003e\n\u003cp\u003ePrepare the working electrophoresis buffer before SDS-PAGE experiments according to laboratory requirements.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDilute 10X Tris-Glycine-SDS Electrophoresis Buffer with deionized water at a 1:9 ratio to prepare 1X running buffer.\u003c\/li\u003e\n\u003cli\u003eMix thoroughly before adding the buffer to the electrophoresis chamber.\u003c\/li\u003e\n\u003cli\u003eLoad prepared protein samples onto Tris-HCl polyacrylamide gels and perform SDS-PAGE according to the selected electrophoresis conditions.\u003c\/li\u003e\n\u003cli\u003eAfter electrophoresis, proteins can be visualized by staining or transferred for Western blot detection.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eIf precipitation occurs after low-temperature storage or transportation, allow the solution to return to room temperature and mix until fully dissolved before use.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nORDERING INFORMATION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eOrdering Information\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eAP0281\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eTris-Glycine-SDS Electrophoresis Buffer (10×)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFAQ\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is Tris-Glycine-SDS Electrophoresis Buffer used for?\u003c\/summary\u003e\n\u003cp\u003eTris-Glycine-SDS Electrophoresis Buffer is a protein running buffer used for SDS-PAGE electrophoresis. It provides the ionic environment required for separating denatured proteins according to molecular weight in Tris-Glycine gel systems.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow do you prepare 1X SDS-PAGE running buffer from 10X Tris Glycine SDS Buffer?\u003c\/summary\u003e\n\u003cp\u003ePrepare 1X running buffer by diluting one part of 10X Tris-Glycine-SDS Electrophoresis Buffer with nine parts of deionized water. For example, mix 100 mL of 10X buffer with 900 mL water to prepare 1 L of working solution.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan this Tris Glycine SDS Running Buffer be used for Western blot?\u003c\/summary\u003e\n\u003cp\u003eYes. This SDS-PAGE running buffer is commonly used for protein separation before Western blot transfer and detection workflows.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat type of gels are compatible with this electrophoresis buffer?\u003c\/summary\u003e\n\u003cp\u003eThis buffer is designed for Tris-HCl polyacrylamide gel systems using the standard Tris-Glycine-SDS electrophoresis method. It is not recommended for HEPES-based gel systems or alternative buffer chemistries.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan used SDS-PAGE running buffer be reused?\u003c\/summary\u003e\n\u003cp\u003eRecovered running buffer may be reused for external chamber electrophoresis applications 1–2 times. However, fresh running buffer is recommended when maximum electrophoresis performance and reproducibility are required.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhy does the buffer sometimes show precipitation?\u003c\/summary\u003e\n\u003cp\u003ePrecipitation may occur after cold storage or transportation because some components can crystallize at low temperatures. Allowing the solution to warm to room temperature and mixing for approximately 10–30 minutes usually restores the solution.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPRODUCT SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!-- =========================\nFAQ SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AccuRef Scientific","offers":[{"title":"500mL","offer_id":52778526605493,"sku":"AP0281","price":33.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_a38b2cde-b134-41a9-9240-6e54afdd0788.jpg?v=1783566115","url":"https:\/\/biofargo.com\/products\/tris-glycine-sds-running-buffer-10x","provider":"Biofargo","version":"1.0","type":"link"}