Description

Tris-Glycine-SDS Running Buffer (10X) is an optimized protein electrophoresis buffer designed for Tris-Glycine SDS-PAGE workflows. During routine protein gel electrophoresis, adjusting voltage conditions between stacking and resolving gels can increase operation time and reduce workflow efficiency. This rapid SDS-PAGE running buffer provides a simplified electrophoresis solution that enables constant voltage operation while maintaining reliable protein separation performance.

Compared with conventional Tris-Glycine SDS running buffer, this 10X electrophoresis buffer allows SDS-PAGE separation under a continuous 180–200 V constant voltage condition, reducing total electrophoresis time to approximately 30–35 minutes. The optimized formulation improves separation efficiency for low molecular weight proteins and enables clearer resolution of closely spaced protein bands, including improved visibility of 10 kDa and 15 kDa prestained protein marker bands when using polyacrylamide gels above 8% concentration.

Tris-Glycine-SDS Running Buffer (10X) is suitable for protein gel electrophoresis, Western blot sample preparation, protein molecular weight analysis, and routine SDS-PAGE applications. It provides a convenient alternative for laboratories requiring fast, reproducible, and efficient protein separation workflows.

Specifications

Product Name	Tris-Glycine-SDS Running Buffer (10X)
SKU	AP1091
Volume	500 mL
Product Type	Rapid Tris-Glycine SDS-PAGE Running Buffer
Buffer System	Tris-Glycine-SDS Electrophoresis System
Working Concentration	1X working solution
Dilution Ratio	10X concentrate diluted 1:10 with deionized water
Recommended Voltage	180–200 V constant voltage
Electrophoresis Time	Approximately 30–35 minutes
Storage	Room temperature
Shelf Life	18 months

Features

Rapid SDS-PAGE electrophoresis: Optimized Tris-Glycine-SDS running buffer enables fast protein separation with approximately 30–35 minutes electrophoresis time.
Constant voltage operation: Supports continuous 180–200 V electrophoresis without requiring separate voltage adjustment between stacking and resolving gel stages.
Improved small protein resolution: Provides enhanced separation performance for low molecular weight proteins and closely positioned protein bands.
Compatible with standard SDS-PAGE workflows: Designed for conventional Tris-Glycine protein electrophoresis systems and Western blot sample analysis.
Convenient 10X concentrate format: Reduces storage space requirements and allows flexible preparation of working electrophoresis buffer.
Reproducible protein separation: Provides consistent electrophoresis conditions for routine protein analysis applications.

Applications

Tris-Glycine-SDS Running Buffer (10X) is widely used as a protein electrophoresis buffer in molecular biology, biochemistry, and life science research workflows.

SDS-PAGE Protein Electrophoresis: Provides running buffer conditions for separation of denatured proteins according to molecular weight.
Western Blot Protein Analysis: Supports protein separation before transfer to PVDF or nitrocellulose membranes for antibody-based detection.
Prestained Protein Marker Analysis: Enables clear visualization of protein molecular weight markers during gel electrophoresis.
Low Molecular Weight Protein Separation: Suitable for analysis of small proteins and closely spaced protein bands requiring improved resolution.
Recombinant Protein Characterization: Used for evaluating recombinant protein expression and molecular weight profiles.
Cell Lysate Protein Analysis: Compatible with protein samples extracted from cultured cells and biological samples.
Protein Molecular Weight Determination: Provides reliable electrophoretic separation for protein size estimation.
High-Throughput Protein Research: Suitable for laboratories requiring faster SDS-PAGE workflows and increased experimental efficiency.

Protocol Overview

Prepare the working electrophoresis buffer before SDS-PAGE experiments.

Dilute Tris-Glycine-SDS Running Buffer (10X) with deionized water at a 1:10 ratio to prepare 1X working solution.
Use the prepared 1X running buffer for standard Tris-Glycine SDS-PAGE electrophoresis systems.
Perform electrophoresis under constant voltage conditions of 180–200 V.
For optimized separation performance, select appropriate polyacrylamide gel concentration according to target protein molecular weight.

Prepared 1X working buffer is recommended to be used within two weeks. If precipitation occurs after low-temperature storage, gently warm the solution until completely dissolved and allow it to return to room temperature before use.

Ordering Information

SKU	Product	Size
AP1091	Tris-Glycine-SDS Running Buffer (10X)	500 mL

FAQ

What is Tris-Glycine-SDS Running Buffer used for?

Tris-Glycine-SDS Running Buffer is an electrophoresis buffer used for SDS-PAGE protein separation. It provides the ionic environment required for protein migration through polyacrylamide gels and is commonly used in Western blot and protein analysis workflows.

What is the difference between 10X and 1X Tris-Glycine SDS running buffer?

The 10X formulation is a concentrated stock solution designed for storage and convenient preparation. Before electrophoresis, it should be diluted with deionized water to prepare the 1X working running buffer.

How fast can SDS-PAGE be completed using this rapid running buffer?

This rapid SDS-PAGE running buffer supports constant voltage electrophoresis at 180–200 V, allowing protein separation to be completed in approximately 30–35 minutes depending on voltage conditions and gel characteristics.

Can this Tris-Glycine SDS buffer be used for Western blot?

Yes. This buffer is suitable for SDS-PAGE protein separation before Western blot transfer and detection workflows.

Does this buffer improve separation of small molecular weight proteins?

Yes. The optimized formulation provides improved resolution for low molecular weight proteins and can enhance separation of closely spaced bands such as 10 kDa and 15 kDa protein marker bands when using suitable gel concentrations.

What voltage should be used with this SDS-PAGE running buffer?

The recommended electrophoresis condition is constant voltage operation at 180–200 V. The exact running time may vary depending on gel percentage, electrophoresis equipment, and sample characteristics.

How should the 10X running buffer be stored?

The concentrated Tris-Glycine-SDS running buffer can be stored at room temperature with an 18-month shelf life. Low-temperature storage may cause temporary precipitation that can be dissolved by gentle warming.

Tris-Glycine-SDS Rapid Electrophoresis Buffer (10×)