{"product_id":"tris-glycine-sds-electrophoresis-buffer-10x","title":"Tris-Glycine-SDS Rapid Electrophoresis Buffer (10×)","description":"\u003c!-- =========================\nPRODUCT DESCRIPTION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eTris-Glycine-SDS Running Buffer (10X) is an optimized protein electrophoresis buffer designed for Tris-Glycine SDS-PAGE workflows. During routine protein gel electrophoresis, adjusting voltage conditions between stacking and resolving gels can increase operation time and reduce workflow efficiency. This rapid SDS-PAGE running buffer provides a simplified electrophoresis solution that enables constant voltage operation while maintaining reliable protein separation performance.\u003c\/p\u003e\n\u003cp\u003eCompared with conventional Tris-Glycine SDS running buffer, this 10X electrophoresis buffer allows SDS-PAGE separation under a continuous 180–200 V constant voltage condition, reducing total electrophoresis time to approximately 30–35 minutes. The optimized formulation improves separation efficiency for low molecular weight proteins and enables clearer resolution of closely spaced protein bands, including improved visibility of 10 kDa and 15 kDa prestained protein marker bands when using polyacrylamide gels above 8% concentration.\u003c\/p\u003e\n\u003cp\u003eTris-Glycine-SDS Running Buffer (10X) is suitable for protein gel electrophoresis, Western blot sample preparation, protein molecular weight analysis, and routine SDS-PAGE applications. It provides a convenient alternative for laboratories requiring fast, reproducible, and efficient protein separation workflows.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nSPECIFICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eTris-Glycine-SDS Running Buffer (10X)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eAP1091\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eVolume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eRapid Tris-Glycine SDS-PAGE Running Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eBuffer System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eTris-Glycine-SDS Electrophoresis System\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eWorking Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e1X working solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eDilution Ratio\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e10X concentrate diluted 1:10 with deionized water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eRecommended Voltage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e180–200 V constant voltage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eElectrophoresis Time\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eApproximately 30–35 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eRoom temperature\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e18 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFEATURES\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRapid SDS-PAGE electrophoresis:\u003c\/strong\u003e Optimized Tris-Glycine-SDS running buffer enables fast protein separation with approximately 30–35 minutes electrophoresis time.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eConstant voltage operation:\u003c\/strong\u003e Supports continuous 180–200 V electrophoresis without requiring separate voltage adjustment between stacking and resolving gel stages.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eImproved small protein resolution:\u003c\/strong\u003e Provides enhanced separation performance for low molecular weight proteins and closely positioned protein bands.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCompatible with standard SDS-PAGE workflows:\u003c\/strong\u003e Designed for conventional Tris-Glycine protein electrophoresis systems and Western blot sample analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eConvenient 10X concentrate format:\u003c\/strong\u003e Reduces storage space requirements and allows flexible preparation of working electrophoresis buffer.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eReproducible protein separation:\u003c\/strong\u003e Provides consistent electrophoresis conditions for routine protein analysis applications.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nAPPLICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eTris-Glycine-SDS Running Buffer (10X) is widely used as a protein electrophoresis buffer in molecular biology, biochemistry, and life science research workflows.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSDS-PAGE Protein Electrophoresis:\u003c\/strong\u003e Provides running buffer conditions for separation of denatured proteins according to molecular weight.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eWestern Blot Protein Analysis:\u003c\/strong\u003e Supports protein separation before transfer to PVDF or nitrocellulose membranes for antibody-based detection.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003ePrestained Protein Marker Analysis:\u003c\/strong\u003e Enables clear visualization of protein molecular weight markers during gel electrophoresis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eLow Molecular Weight Protein Separation:\u003c\/strong\u003e Suitable for analysis of small proteins and closely spaced protein bands requiring improved resolution.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Protein Characterization:\u003c\/strong\u003e Used for evaluating recombinant protein expression and molecular weight profiles.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCell Lysate Protein Analysis:\u003c\/strong\u003e Compatible with protein samples extracted from cultured cells and biological samples.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein Molecular Weight Determination:\u003c\/strong\u003e Provides reliable electrophoretic separation for protein size estimation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHigh-Throughput Protein Research:\u003c\/strong\u003e Suitable for laboratories requiring faster SDS-PAGE workflows and increased experimental efficiency.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPROTOCOL OVERVIEW\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eProtocol Overview\u003c\/h2\u003e\n\u003cp\u003ePrepare the working electrophoresis buffer before SDS-PAGE experiments.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDilute Tris-Glycine-SDS Running Buffer (10X) with deionized water at a 1:10 ratio to prepare 1X working solution.\u003c\/li\u003e\n\u003cli\u003eUse the prepared 1X running buffer for standard Tris-Glycine SDS-PAGE electrophoresis systems.\u003c\/li\u003e\n\u003cli\u003ePerform electrophoresis under constant voltage conditions of 180–200 V.\u003c\/li\u003e\n\u003cli\u003eFor optimized separation performance, select appropriate polyacrylamide gel concentration according to target protein molecular weight.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003ePrepared 1X working buffer is recommended to be used within two weeks. If precipitation occurs after low-temperature storage, gently warm the solution until completely dissolved and allow it to return to room temperature before use.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nORDERING INFORMATION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eOrdering Information\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eAP1091\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eTris-Glycine-SDS Running Buffer (10X)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFAQ\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is Tris-Glycine-SDS Running Buffer used for?\u003c\/summary\u003e\n\u003cp\u003eTris-Glycine-SDS Running Buffer is an electrophoresis buffer used for SDS-PAGE protein separation. It provides the ionic environment required for protein migration through polyacrylamide gels and is commonly used in Western blot and protein analysis workflows.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the difference between 10X and 1X Tris-Glycine SDS running buffer?\u003c\/summary\u003e\n\u003cp\u003eThe 10X formulation is a concentrated stock solution designed for storage and convenient preparation. Before electrophoresis, it should be diluted with deionized water to prepare the 1X working running buffer.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow fast can SDS-PAGE be completed using this rapid running buffer?\u003c\/summary\u003e\n\u003cp\u003eThis rapid SDS-PAGE running buffer supports constant voltage electrophoresis at 180–200 V, allowing protein separation to be completed in approximately 30–35 minutes depending on voltage conditions and gel characteristics.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan this Tris-Glycine SDS buffer be used for Western blot?\u003c\/summary\u003e\n\u003cp\u003eYes. This buffer is suitable for SDS-PAGE protein separation before Western blot transfer and detection workflows.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes this buffer improve separation of small molecular weight proteins?\u003c\/summary\u003e\n\u003cp\u003eYes. The optimized formulation provides improved resolution for low molecular weight proteins and can enhance separation of closely spaced bands such as 10 kDa and 15 kDa protein marker bands when using suitable gel concentrations.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat voltage should be used with this SDS-PAGE running buffer?\u003c\/summary\u003e\n\u003cp\u003eThe recommended electrophoresis condition is constant voltage operation at 180–200 V. The exact running time may vary depending on gel percentage, electrophoresis equipment, and sample characteristics.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow should the 10X running buffer be stored?\u003c\/summary\u003e\n\u003cp\u003eThe concentrated Tris-Glycine-SDS running buffer can be stored at room temperature with an 18-month shelf life. Low-temperature storage may cause temporary precipitation that can be dissolved by gentle warming.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPRODUCT SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!-- =========================\nFAQ SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AccuRef Scientific","offers":[{"title":"500mL","offer_id":52778529620149,"sku":"AP1091","price":57.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_5ea7a70b-10a0-4066-8a0d-f3beb12b945f.jpg?v=1783566537","url":"https:\/\/biofargo.com\/products\/tris-glycine-sds-electrophoresis-buffer-10x","provider":"Biofargo","version":"1.0","type":"link"}