Synonym(s): N-[Tris(hydroxymethyl)methyl]glycine, Tricine, ULTROL® Grade, Tricine
Linear Formula: (HOCH2)3CNHCH2CO2H

Description

In biological experiments, it is an important pH stabilizing reagent. Typically, a suitable weak acid is mixed with its conjugate base to obtain the appropriate pH value. Most biological reactions occur under neutral conditions, generally with a pH between 6 and 8, so the effective buffering range of the buffer is also required to be between 6 and 8. In addition, it is required that the acidic and basic forms of the buffer do not chelate with certain metal ions.

Specifications

Item	Value
Chemical Name	N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine
Appearance	White Crystalline Powder
CAS No.	5704-04-1
Molecular Formula	C₆H₁₃NO₅
Molecular Weight	179.2
Grade	Biochemical Grade
Purity	≥99.0%
Loss on Drying	≤1.0%
Water Solubility	Clear
pH	4.0-6.0
Heavy Metals	≤5ppm
UV Absorbance (260nm, 1mol/L, 1cm)	≤0.1
UV Absorbance (280nm, 1mol/L, 1cm)	≤0.05
Storage / Handling	Store at Room Temperature.

Features

High-purity biochemical buffer (≥99%) ensuring reliable performance in sensitive biological and biochemical assays.
Excellent buffering capacity in the pH 6.0–8.0 range, making it ideal for experiments requiring near-neutral and stable conditions.
Low UV absorbance at 260 nm and 280 nm, supporting applications that require minimal optical interference such as nucleic acid and protein detection.
High water solubility with clear solution formation, simplifying buffer preparation and ensuring consistent results.
Minimal metal-ion chelation, reducing interference in metalloprotein studies, enzyme assays, and electrophoretic separations.
Stable and non-reactive crystalline form, compatible with long-term storage and repeated laboratory use.

Applications

Electrophoresis (especially Tricine-SDS-PAGE)

Ideal for resolving low-molecular-weight proteins and peptides due to its superior buffering behavior at lower molecular weight ranges.
Protein and enzyme research

Maintains stable reaction conditions with minimal metal-ion interaction, suitable for enzymatic assays, kinetic studies, and protein purification.
Cell-free systems & biochemical reactions

Supports in vitro transcription/translation, metabolic assays, and reactions requiring strict pH stability around neutrality.
Nucleic acid–related workflows

Low UV background enhances accuracy in DNA/RNA quantification and optical detection methods.
Molecular biology buffers & reagent preparation

Frequently used in preparing running buffers, sample buffers, dilution buffers, and reaction systems needing consistent and reliable pH control.

Safety Disclaimer

This product is intended for laboratory research use only. Not for drug, human, or veterinary use. Review the Safety Data Sheet (SDS) provided by BioFargo for detailed handling, storage, hazard, and disposal information. Use appropriate personal protective equipment (PPE) including gloves, lab coat, and eye protection when handling. Dispose of waste and residual buffer in accordance with local, state, and federal regulations. Ensure proper ventilation if preparing large volumes or heating solutions.

Documents

SDS

COA

Protocol

TRICINE 99% Biochemical Grade