{"product_id":"tri-color-prestained-protein-marker-10-180-kda","title":"Tri-Color Prestained Protein Marker (10–180 kDa)","description":"\u003c!-- =========================\nPRODUCT DESCRIPTION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eTri-Color Prestained Protein Marker (10–180 kDa) is a ready-to-use protein molecular weight marker designed for accurate protein size estimation during SDS-PAGE and Western blot workflows. In protein electrophoresis experiments, unclear band positioning, inconsistent molecular weight estimation, and difficulty monitoring membrane transfer can affect downstream protein analysis. A reliable prestained protein ladder provides researchers with a visible reference standard to evaluate protein migration and transfer performance.\u003c\/p\u003e\n\u003cp\u003eThis tri-color prestained protein marker contains 10 colored protein bands covering a broad molecular weight range from 10 kDa to 180 kDa. The marker produces clear visual bands directly during gel electrophoresis and after transfer onto PVDF or nitrocellulose (NC) membranes, making it suitable as a protein molecular weight marker for Western blotting and SDS-PAGE applications.\u003c\/p\u003e\n\u003cp\u003eThe three-color design includes a green 10 kDa reference band, a red 70 kDa reference band, and blue bands for the remaining molecular weight positions. Compared with traditional single-color protein ladders, this multicolor protein marker enables faster identification of protein regions and improves experimental convenience in routine molecular biology workflows.\u003c\/p\u003e\n\u003cp\u003eTri-Color Prestained Protein Marker is supplied as a ready-to-use liquid formulation that requires no heating, dilution, or addition of reducing agents before loading. It is suitable for laboratories performing protein electrophoresis, recombinant protein analysis, and Western blot detection.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nSPECIFICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eTri-Color Prestained Protein Marker (10–180 kDa)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eRP0011S\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eVolume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e250 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eProduct Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003ePrestained Protein Molecular Weight Marker\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eMolecular Weight Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e10–180 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eNumber of Bands\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e10 prestained protein bands\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eColor Reference Bands\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e10 kDa Green Band; 70 kDa Red Band; Other Bands Blue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eSDS-PAGE, Western Blot, Protein Electrophoresis, Molecular Weight Estimation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eMembrane Compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003ePVDF Membrane, Nitrocellulose (NC) Membrane\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eAt least 2 years\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFEATURES\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTri-color prestained protein ladder:\u003c\/strong\u003e Provides clearly distinguishable colored bands for rapid protein migration monitoring and molecular weight estimation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBroad molecular weight coverage:\u003c\/strong\u003e Contains 10 protein bands ranging from 10 kDa to 180 kDa, suitable for a wide range of protein analysis applications.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eReady-to-use formulation:\u003c\/strong\u003e Direct loading without heating, dilution, or additional reducing agents simplifies SDS-PAGE sample preparation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCompatible with SDS-PAGE and Western blot:\u003c\/strong\u003e Enables visualization during electrophoresis and after transfer to PVDF or NC membranes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eStable long-term performance:\u003c\/strong\u003e Maintains reliable band quality during long-term storage at -20 °C.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eEasy protein size identification:\u003c\/strong\u003e Dedicated colored reference bands help researchers quickly locate target protein regions.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nAPPLICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eTri-Color Prestained Protein Marker is widely used as a protein electrophoresis standard for molecular biology, biochemical research, and protein analysis workflows.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eWestern Blot Protein Detection:\u003c\/strong\u003e Used as a prestained Western blot protein marker to monitor protein transfer efficiency and estimate target protein molecular weight after membrane transfer.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSDS-PAGE Protein Electrophoresis:\u003c\/strong\u003e Provides visible molecular weight references during gel electrophoresis for protein separation and analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein Molecular Weight Estimation:\u003c\/strong\u003e Helps determine approximate sizes of unknown proteins by comparing sample bands with known marker positions.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Protein Analysis:\u003c\/strong\u003e Supports verification of recombinant protein expression and purification results.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCell Lysate Protein Analysis:\u003c\/strong\u003e Suitable for evaluating protein profiles from cultured cells and biological samples.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein Transfer Monitoring:\u003c\/strong\u003e Allows researchers to visually confirm protein migration from gel to PVDF or NC membranes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein Electrophoresis Teaching and Training:\u003c\/strong\u003e Suitable for educational laboratories requiring clear visualization of protein standards.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPROTOCOL OVERVIEW\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eProtocol Overview\u003c\/h2\u003e\n\u003cp\u003eTri-Color Prestained Protein Marker is supplied as an immediate-use protein ladder for SDS-PAGE and Western blot workflows.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eGently mix the marker solution before use to ensure uniform distribution of protein bands.\u003c\/li\u003e\n\u003cli\u003eLoad 3–5 μL of the marker directly into the SDS-PAGE gel.\u003c\/li\u003e\n\u003cli\u003ePerform electrophoresis according to standard protein separation procedures.\u003c\/li\u003e\n\u003cli\u003eVisualize colored marker bands during electrophoresis or after transfer onto PVDF\/NC membranes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eNo heating, dilution, or addition of reducing agents is required before loading.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nORDERING INFORMATION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eOrdering Information\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eRP0011S\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eTri-Color Prestained Protein Marker (10–180 kDa)\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e250 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFAQ\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is a prestained protein marker used for?\u003c\/summary\u003e\n\u003cp\u003eA prestained protein marker is used as a visible molecular weight standard during SDS-PAGE and Western blot experiments. It helps researchers estimate protein size, monitor protein migration, and evaluate transfer efficiency during membrane transfer.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat molecular weight range does this protein ladder cover?\u003c\/summary\u003e\n\u003cp\u003eTri-Color Prestained Protein Marker covers a molecular weight range from 10 kDa to 180 kDa and contains 10 colored protein bands suitable for common protein analysis workflows.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan this protein marker be used for Western blot?\u003c\/summary\u003e\n\u003cp\u003eYes. This Western blot protein marker can be visualized after transfer onto PVDF or nitrocellulose membranes and is commonly used for monitoring protein transfer and estimating target protein molecular weight.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes the protein ladder require heating before loading?\u003c\/summary\u003e\n\u003cp\u003eNo. This ready-to-use protein ladder does not require heating, dilution, or addition of reducing agents before SDS-PAGE loading. Simply mix gently and load the recommended volume.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat are the colored bands in this tri-color protein marker?\u003c\/summary\u003e\n\u003cp\u003eThe marker contains a green 10 kDa reference band, a red 70 kDa reference band, and blue bands for the remaining molecular weight positions, allowing rapid identification during electrophoresis.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow much protein marker should be loaded for SDS-PAGE?\u003c\/summary\u003e\n\u003cp\u003eThe recommended loading volume is approximately 3–5 μL per lane. The optimal amount may vary depending on gel percentage, imaging conditions, and experimental requirements.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow should the prestained protein ladder be stored?\u003c\/summary\u003e\n\u003cp\u003eThe protein molecular weight marker should be stored at -20 °C. Under recommended storage conditions, the product remains stable for at least two years.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPRODUCT SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!-- =========================\nFAQ SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AccuRef Scientific","offers":[{"title":"250μL","offer_id":52776570093749,"sku":"RP0011S","price":87.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_a2df3de7-51f8-436c-af7d-71d3b37d32ea.jpg?v=1783499244","url":"https:\/\/biofargo.com\/products\/tri-color-prestained-protein-marker-10-180-kda","provider":"Biofargo","version":"1.0","type":"link"}