Description

This kit can extract whole proteins from mammalian tissues and cultured cells. Lysis Buffer homogenate containing a mixture of protease inhibitors and phosphatase inhibitors is used to lyse tissues or cells, with a mild effect and a simple and efficient extraction process. It can extract the vast majority of proteins from cultured cells or animal tissues. Proteins can be extracted from 107 cultured cells or 200 mg of animal tissue at a time.

Specification

Parameter	Specification
Package	50 ASSAYS
Full name	Tissue or Cell Total Protein Extraction
Composition	Lysis buffer, Protease inhibitors, Phosphatase inhibitors, PMSF
Component	Lysis buffer, Protease inhibitors, Phosphatase inhibitors, PMSF

Features

The extracted proteins include membrane, nucleus, nuclear matrix, and cytoplasmic proteins.
The entire operation process only takes about 30 minutes.
It can be used to culture cells (50 × 10 ^ 7), can also be used in animal tissues (50 × 200 mg) protein extraction.
Avoid protein degradation by proteases and phosphatases, maintain protein integrity and natural activity.
No need for high-speed centrifugation, multiple samples can be processed simultaneously.

Applications

The extracted whole protein can be used for Western Blot, immunoprecipitation, enzyme activity analysis, Pull down, EMSA and other subsequent studies, but cannot be used for protein kinase and phosphatase immunoprecipitation studies (inhibitors of these two enzymes are included in this kit).

Storage

Store separately according to label prompts

Tissue or Cell Total Protein Extraction