Description

Tiger Puffer Nervous Necrosis Virus (TPNNV) Probe Realtime RT-PCR Kit is designed for the quantitative detection of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples.

Key Features

One-tube qPCR format minimizes carry-over contamination risk

High sensitivity: analytical LOD as low as 100 copies/reaction
Excellent specificity: no cross-reactivity with DNA from other pathogens
Includes amplification positive control for result verification
Compatible with various DNA purification methods
Suitable for both qualitative and quantitative applications
For quantitative use: the linear range covers a 100,000-fold concentration difference.
Sufficient for 50 reactions (20 μL each)
For Research Use Only (RUO)

Kit Components

Component Name	Product Number.	Size	Vial	Lid Color
Probe qRT-PCR Buffer	990504a	500 μL	0.5 μL Vial	Blue
Probe qRT-PCR Enzyme	990504bv2	50 μL	0.5 mL Vial	Red
Template Dilution Buffer	180701	1 mL	1.5 mL Vial	Green
Primer-Probe Powder	yp15-55220	50 T	1.5 mL Brown Vial	Brown
Amplification Positive Control (1E7 copies/μL)	pc55220	0.5 mL	0.5 mL Vial	Yellow
Manual	sc15-55220	1 Set	NA	NA

Application Areas

Aquaculture disease surveillance and biosecurity: rapid screening and viral load quantification of Tiger Puffer Nervous Necrosis Virus (TPNNV) in farmed fish (e.g., tiger puffer, grouper, sea bass) for early outbreak detection, treatment/vaccine efficacy monitoring, and selective-breeding programs.
Environmental and wild-population pathogen monitoring: quantitative detection of TPNNV RNA in seawater, sediment and wild fish samples to support marine ecosystem health assessments, epidemiological surveillance and risk-based management.
Diagnostic development and laboratory research: quantitative RT‑PCR for assay validation, diagnostic R&D, host–pathogen interaction studies and vaccine development/testing in finfish model systems.

Storage & Shipping

Store at - 20 °C
The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.

FAQ

Q: Are Biofargo PCR and qPCR Kits compatible with my thermal cycler or real-time PCR instrument?

A: Yes. Biofargo PCR Kits and probe-based qPCR Kits are designed for broad compatibility with mainstream instruments.

PCR Kits can be used with standard thermal cyclers from leading brands.

Probe-based qPCR Kits are fully compatible with major real-time PCR platforms, including ABI, Bio-Rad, Roche, QuantStudio, Qiagen Rotor-Gene Q (5-plex), and Genesig Q16.

Our technical team is available to assist with instrument setup and run optimization to ensure the best results.

Documents

Tiger Puffer Nervous Necrosis Virus (TPNNV) Probe Realtime RT-PCR Kit User Manual

MSDS