Description

The EndoFree Midi Plasmid Kit adopts a unique silica membrane adsorption technique to efficiently and specifically bind plasmid DNA.The special Buffer EBT and the Filtration CS1 can effectively remove impurities such as endotoxin and protein. The entire extraction process takes only 1 hour, which is convenient and quick.

Features

Suitable for extracting plasmids from 50-100 ml bacteria culture medium.
The purified plasmid contain very low endotoxin and can meet the requirements of high quality transfection.
Easy to operate with a unique and rapid endotoxin removal method.

Storage

Room temperature (15-25℃ ) in dry condition

Applications

The purified plasmid DNA can be applied in molecular biology experiments and cell biology experiments such as restriction endonuclease digestion,sequencing, transformation, transfection, microinjection, etc.

Plasmid Yield

Plasmid type	Bacterial cells volume	Plasmid yield
High copy	50 mL	250-750 μg
Low copy	100 mL	100-300 μg

Purification Workflow

Documents

DP108-EndoFree Midi Plasmid Kit-190123

DP203-TIANquick Mini Purification Kit-121221

TIANprep Midi Plasmid Kit -Tiangen