Description

The TIANgel Purification Kit adopts a unique adsorption column to effectively recover DNA fragments from agarose gel in either TAE or TBE buffer, while removing impurities such as proteins, ions and small primers. 100 bp-15 kb DNA fragments can be recovered with the recovery rate up to 80%. The maximal amount of DNA that can be adsorbed by each adsorption column is 10 μg.

Features

Fast: The entire operation process is fast and convenient, and can be completed in around 10 min.
Convenient: Gel blocks can be dissolved at room temperature without the use of balance buffer.
High efficient: The unique spin column and buffers ensure maximal recovery of high-purity target DNA.

Applications

The purified DNA can be directly used for regular experiments such as restriction enzyme digestion, PCR, sequencing, library screening, ligation, transformation, etc.

Required Reagents

Ethanol

Storage

This kit can be stored dry for 12 months under room temperature (15-25℃ ). For longterm storage, store at 2-8℃ . When stored at 2-8℃ , precipitation might be formed in buffer. Place the buffers at room temperature for a period of time before use, and if necessary, incubate the buffers in a 37 ℃ water bath for 10 minutes to dissolve the precipitation.

Documents

GDP219-TIANgel Purification Kit-250318

DP209-TIANgel Midi Purification Kit-190123

TIANgel Purification Kit-Tiangen