Description

The TIANamp Bacteria DNA Kit adopts a highly efficient, DNA-specific adsorption spin column and a unique buffer system for both genomic DNA extraction from Gram-negative and Gram-positive bacteria. The kit can also be used for the extraction of pathogenic bacteria (microorganisms) of food, such as Staphylococcus aureus, cholera sclerotia, hemorrhagic Escherichia coli O157:H7, Listeria monocytogenes, Salmonella, Enterobacter sakazakii, etc. 10-40 μl of pure genomic DNA can be rapidly extracted and purified from 1-5 ml of bacterial culture medium, and the obtained genomic DNA can be directly used in molecular biology experiments such as PCR template, restriction enzyme digestion and Southern blot.

Features

Simple and fast: High purity genomic DNA of Gram-negative bacteria can be obtained within 1 hour
Excellent quality: The purified DNA can be directly used in downstream molecular experiments such as PCR, restriction endonuclease digestion, Southern blotting, etc.

Required Reagents

Lysozyme (Gram-positive bacteria), ethanol, lysozyme buffer (20 mM Tris, pH8.0; 2 mM Na2-EDTA, 1.2% Triton-100)

Applications

Genome extraction of Gram-negative bacteria
Genome extraction of Gram-positive bacteria
Genome extraction of pathogenic bacteria in food

DNA Extraction Yield From Various Plant Tissues

Bacteria Type	Gram-negative bacteria(such as E.coli)	Gram-positive bacteria(such as Glucococcus epidermidis)
Bacteria Concentration	2x 10⁸ cells/mL	3.5x 10⁸ cells/mL
Culture Volume	1 mL	1 mL
DNA Yield	15-20 μg	6-13 μ9
OD₂₆₀/OD₂₈₀	1.7-1.9	1.7-1.9

Note: The DNA extraction amount may vary depending on the bacteria types and culture time, etc. Gram positive bacteria require special treatments such as lysozyme for lysing, and the genomic DNA extraction can be performed according to the procedures of Gram-negative bacteria.

Storage

Room temperature (15-25℃)

Documents

GDP302-TIANamp Bacteria DNA Kit-250318

Publications

Solobacterium moorei promotes the progression of adenomatous polyps by causing inflammation and disrupting the intestinal barrier.

Publication: Journal of Translational Medicine

TIANamp Bacteria DNA Kit-Tiangen