Description

THUNDERBIRD™ Probe and SYBR® qPCR Mix is a highly efficient 2x Master Mix for real-time PCR using TaqMan™ probes and SYBR® Green I. The master mix contains all required components, except for ROX reference dye, probe and primers (50x ROX reference dye is individually supplied with this kit). The master mix facilitates reaction setup, and improves the reproducibility of experiments.
These products are improved versions of Realtime PCR Master Mix (Code No. QPK-101) and Realtime PCR Master Mix (Code No. QPK-201) . In particular, reaction specificity and PCR efficiency is enhanced.

This product is sold in U.S. under the license of US patent 7772383
from Chakrabarti Advanced Technology NewCo LLC.

Features

• The specificity for the detection of low-copy targets is improved.
• The dispersion of PCR efficiency between targets is reduced by a new PCR enhancer*. (*Patent pending)
• High specificity and effective amplification enable the detection of a broad dynamic range.
• The reagent is applicable to most real-time cyclers (i.e. Block type and glass capillary type). Because the 50x ROX reference dye is individually supplied with this kit, the kit can be applied to real-time cyclers that require a passive reference dye.
• The master mix contains anti-Taq DNA polymerase antibodies for hot start technology. The antibodies are easily inactivated in the first denaturation step, thereby activating the DNA polymerase.

Specification

Category	Item	Details
Storage condition	-	Store at -20ºC

Components

QPS-101T	Details
THUNDERBIRD™ Probe qPCR Mix	1 mL × 1
50x ROX reference dye	50 µL × 1

QPS-101	Details
THUNDERBIRD™ Probe qPCR Mix	1.67 mL × 3
50x ROX reference dye	250 µL × 1

QPS-201T	Details
THUNDERBIRD™ SYBR® qPCR Mix	1 mL × 1
50x ROX reference dye	50 µL × 1

QPS-201	Details
THUNDERBIRD™ SYBR® qPCR Mix	1.67 mL × 3
50x ROX reference dye	250 µL × 1

Compatible Real-Time Instruments

Manufacturer	Instrument	Manufacturer	Instrument
Applied Biosystems	ABI PRISM™ 7000	Roche Diagnostics	LightCycler™ 1.x / 2.0
Applied Biosystems	ABI PRISM™ 7700	Roche Diagnostics	LightCycler™ Nano
Applied Biosystems	Applied Biosystems™ 7300	Roche Diagnostics	LightCycler™ 480
Applied Biosystems	Applied Biosystems™ 7500/7500FAST	Bio-Rad / MJ	MiniOpticon™
Applied Biosystems	Applied Biosystems™ 7900HT	Bio-Rad / MJ	CFX96 Touch™
Applied Biosystems	Applied Biosystems™ StepOne™	Agilent Technologies	Mx3000P / Mx3005P / Mx4000
Applied Biosystems	Applied Biosystems™ StepOnePlus™	TaKaRa	Thermal Cycler Dice™ Real Time Systems

Application Data

Example 1. Comparison of the sensitivity and efficiency of the SYBR® Green I assay

β-actin mRNA was detected with serially diluted cDNA from HeLa cell total RNA. THUNDERBIRD™ SYBR® qPCR Mix [Code No. QPS-201] showed greater sensitivity and efficiency than other kits (companies A and B).

Example 2. Comparison of the PCR efficiency

Norovirus G1 and human GAPDH genes were detected using serially diluted cDNA samples by SYBR® Green I and TaqMan real-time PCR. THUNDERBIRD™ qPCR Master Mix showed greater efficiency than other reagents.

Detection of Norovirus G1 gene by SYBR® Green I assay.
[Roche Diagnostics LightCycler 1.1]

Detection of GAPDH gene by TaqMan™ assay.
[Roche Diagnostics LightCycler 1.1]

Example 3. Verification of the measurement accuracy

Human GAPDH genes were detected using serially 20.5 fold diluted cDNA synthesised from HeLa cell total RNA by SYBR® Green I assay. THUNDERBIRD™ qPCR Master Mix successfully detected the differences between dilutions.

Detection of human GAPDH gene by SYBR® GREEN I assay.
[Applied Biosystems 7900HT]

Documents

Download Manual Download SDS

THUNDERBIRD SYBR qPCR Mix-Toyobo