{"product_id":"thunderbird-next-sybr-qpcr-mix","title":"THUNDERBIRD Next SYBR qPCR Mix-Toyobo","description":"\u003cdiv style=\"font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif; color: #333; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 10px;\"\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDescription\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #f9f9f9; padding: 20px; border-radius: 4px; border: 1px solid #eee;\"\u003e\n\u003cp style=\"margin-top: 0;\"\u003eTHUNDERBIRD™ Next SYBR® qPCR Mix is a highly efficient 2× Master Mix for real-time PCR using SYBR® Green I. The master mix contains all required components, except primers. The master mix facilitates reaction setup, and improves the reproducibility of experiments.\u003c\/p\u003e\n\u003cp style=\"margin-top: 0;\"\u003eThis product is an improved version of THUNDERBIRD™ SYBR® qPCR Mix (Code No. QPS-201). In particular, the reaction specificity and PCR efficiency is enhanced.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eFeatures\u003c\/h2\u003e\n\u003cul style=\"list-style-type: none; padding-left: 0;\"\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e \u003cstrong\u003eHigh specificity\u003c\/strong\u003e\u003cbr\u003eThe specificity for the detection of low-copy targets is improved.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e \u003cstrong\u003eHomogeneous amplification\u003c\/strong\u003e\u003cbr\u003eThe dispersion of PCR efficiency between targets is reduced by a new PCR enhancer.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e \u003cstrong\u003eBroad dynamic range\u003c\/strong\u003e\u003cbr\u003eHigh specificity and effective amplification enable the detection of a broad dynamic range.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e \u003cstrong\u003eCompatibility for various real-time cyclers.\u003c\/strong\u003e\u003cbr\u003eThe reagent is applicable to most real-time cyclers (i.e. Block type and glass capillary type). Because the passive reference dye is included with this kit, the kit can be applied to real-time cyclers that require a passive reference dye.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e \u003cstrong\u003eFast PCR\u003c\/strong\u003e\u003cbr\u003eThis reagent enables amplification using fast cycle condition.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e \u003cstrong\u003eUtilization of dUTP\u003c\/strong\u003e\u003cbr\u003eThis master mix contains dUTP instead of dTTP. Therefore, the rate of false-positive detection can be reduced by adding uracil-N-glycosylase (UNG). *UNG is not supplied with this kit. Uracil-DNA Glycosylase (UNG), Heat-labile (Code No.UNG-101) can be used.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e \u003cstrong\u003eUtilization of a visible tracking dye\u003c\/strong\u003e\u003cbr\u003eThis master mix contains visible tracking dye. This dye helps to eliminate pipetting errors, and does not spectrally overlap with fluorescent dyes used for qPCR and will not interfere with real-time detection.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_r-pcr-011_01.png?v=1775525066\" style=\"max-width: 100%; height: auto;\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eApplication\u003c\/h2\u003e\n\u003cp style=\"padding-left: 5px;\"\u003eIntercalation assay with SYBR® Green I using DNA template\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eSpecification\u003c\/h2\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 600px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eCategory\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eItem\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; font-weight: bold; background-color: #fcfcfc;\"\u003eStorage condition\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: center;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eStore at -20℃, with blocking the light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eComponents\u003c\/h2\u003e\n\u003cp\u003eThis kit includes the following components for 100 reactions (QPX-201T) and 500 reactions (QPX-201), with a total of 20μL per reaction. All reagents should be stored at -20°C.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 600px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eQPX-201T\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eTHUNDERBIRD™ Next SYBR® qPCR Mix\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 600px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eQPX-201\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eTHUNDERBIRD™ Next SYBR® qPCR Mix\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e1.67 mL x 3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003eNote:\u003cbr\u003eTHUNDERBIRD™ Next SYBR® qPCR Mix can be stored, protected from light, at 2-8°C for up to 3 months. For longer storage, this reagent should be kept at -20°C and protected from light. No negative effect was detected by 10 freeze-thaw cycles of THUNDERBRID™ Next SYBR® qPCR Mix.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eApplication Data\u003c\/h2\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 1.Amplification of the 100~500bp region\u003c\/h3\u003e\n\u003cp\u003eUsing artificial gene as template and forward primer as common, reverse primer was designed so that the amplification product lengths were 100bp, 200bp, 300bp, 400bp and 500bp. We used these primers to amplify 107 to 10 copies using THUNDERBIRD™ Next\u003cbr\u003eSYBR® qPCR Mix and other companies to compare the PCR efficiency for each targets. As a result, the amplification efficiency may decrease or be undetectable as the target length increases in other companies, but stable PCR efficiency was achieved with THUNDERBIRD™ Next SYBR® qPCR Mix.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_r-pcr-011_02.png?v=1775525232\" alt=\"\" style=\"max-width: 100%; height: auto;\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 2.Amplification from 1 copy gene\u003c\/h3\u003e\n\u003cp\u003eIf one copy target can be amplified, the number of detected copies would be equivalent to the expected number of detected copies from the Poisson distribution. When one copy is added, the theoretical value from the Poisson distribution is 37% of the probability of having 0 copies and 63% of the probability of having one or more copies.\u003cbr\u003eTHUNDERBIRD™ Next SYBR® qPCR Mix was used to detect 96 samples using Salmonella genome diluted to one copy as a template.\u003cbr\u003eThe results showed that 38.5% of the samples were undetected and 61.5% were detected, which is equivalent to the expected number of samples from the Poisson distribution, suggesting that one copy equivalent can be detected.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_r-pcr-011_03.png?v=1775525232\" alt=\"\" style=\"max-width: 100%; height: auto;\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 3.Amplification of 65bp domain of G3PDH\u003c\/h3\u003e\n\u003cp\u003ecDNA from total RNA of Hela cells synthesized by reversetranscription reagents (Code No. FSQ-101) was used to amplify a 65bp G3PDH gene at 5-fold dilutions of cDNA. As a result, nonspecific amplification occurred in the low copy range in other companies, but nonspecific amplification was not observed by using THUNDERBIRD™ Next SYBR® qPCR Mix, allowing accurate quantification to the low copy range.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_r-pcr-011_04.png?v=1775525233\" alt=\"\" style=\"max-width: 100%; height: auto;\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 4.Comparison of normal cycle and high-speed cycle\u003c\/h3\u003e\n\u003cp\u003eUsing cDNA from Hela cells total RNA synthesized by reverse-transcription reagents (Code No. FSQ-101), amplification of β-actin gene (316bp) was amplified by \"normal cycle with 30sec. extension \" and \"high-speed cycle with 10sec. extension\". As a result, other company products that recommend normal cycling could not be amplified efficiently by high-speed cycling, but THUNDERBIRD™ Next SYBR® qPCR Mix could be amplified efficiently by high-speed cycling.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_r-pcr-011_05.png?v=1775525233\" alt=\"\" style=\"max-width: 100%; height: auto;\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 5.Stability test of the prepared PCR reaction solution\u003c\/h3\u003e\n\u003cp\u003ePrimers and template (cDNA from Hela cells total RNA) were mixed into the PCR-reaction solution, and amplifications of the targets were performed immediately or after standing for 48 hours at a light-shielding room temperature. As a result, Ct values decreased after 48 hours in our conventional kit and other products. But in THUNDERBIRD™ Next SYBR® qPCR Mix, Ct values remained stable even after 48 hours.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_r-pcr-011_06.png?v=1775525233\" alt=\"\" style=\"max-width: 100%; height: auto;\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 6.Prevention of false positives\u003c\/h3\u003e\n\u003cp\u003eTo confirm UNG treatment for preventing carry-over contamination. PCR products containing dUTP (104 copies) were used as template, THUNDERBIRD™ Next SYBR® qPCR Mix and Uracil-DNA Glycosylase(UNG), Heat-labile(Code No. UNG-101) were added, and amplification of the same target was performed by real-time PCR. As a result, we were able to confirm that the first PCR products were degraded by UNG treatment completely.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_r-pcr-011_07.png?v=1775525233\" alt=\"\" style=\"max-width: 100%; height: auto;\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDocuments\u003c\/h2\u003e\n  \u003cdiv style=\"border: 1px solid #eee; background-color: #f9f9f9; padding: 24px; border-radius: 8px;\"\u003e\n\u003cdiv style=\"display: flex; flex-wrap: wrap; gap: 15px;\"\u003e\n\u003ca style=\"display: inline-block; background-color: #003366; color: #ffffff; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold;\" rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/QPX-201_E.pdf?v=1775801243\" target=\"_blank\"\u003e Download Manual \u003c\/a\u003e \n  \u003ca style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/QPX-201_SDS.pdf?v=1775801243\" target=\"_blank\"\u003e Download SDS \u003c\/a\u003e\n\u003ca style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" rel=\"noopener noreferrer\" href=\"https:\/\/biofargo.com\/pages\/contact-us?srsltid=AfmBOorrYySVXlrC6mOen85FBiTKooBmPEyrEjjsqXyC0kKPRm7fDFPV\" target=\"_blank\"\u003e Request COA \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n","brand":"Toyobo","offers":[{"title":"100Rxn","offer_id":52417325695157,"sku":"QPX-201T","price":48.32,"currency_code":"USD","in_stock":true},{"title":"500Rxn","offer_id":52417325727925,"sku":"QPX-201","price":189.6,"currency_code":"USD","in_stock":true},{"title":"2500Rxn","offer_id":52417325760693,"sku":"QPX-201X5","price":853.2,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/THUNDERBIRD_Next_SYBR_qPCR_Mix-Toyobo.jpg?v=1777517164","url":"https:\/\/biofargo.com\/products\/thunderbird-next-sybr-qpcr-mix","provider":"Biofargo","version":"1.0","type":"link"}