{"product_id":"thunderbird-next-probe-qpcr-mix","title":"THUNDERBIRD Next Probe qPCR Mix-Toyobo","description":"\u003cdiv style=\"font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif; color: #333; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 10px;\"\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDescription\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #f9f9f9; padding: 20px; border-radius: 4px; border: 1px solid #eee;\"\u003e\n\u003cp style=\"margin-top: 0;\"\u003eTHUNDERBIRD™ Next Probe qPCR Mix is a 2x concentration premixed reagent for real-time PCR using various detection systems such as TaqMan™ probes and fluorescently labeled primers. The components other than ROX (provided separately), probes, and primers are pre-mixed, making the preparation of the reaction solution convenient. Additionally, this kit minimizes variation in fluorescence intensity between samples, allowing for highly reproducible results.\u003c\/p\u003e\n\u003cp style=\"margin-top: 0;\"\u003eThis product has further improved the composition of the conventional THUNDERBIRD™ Probe qPCR Mix [Code No. QPS-101], optimizing it for multiplex detection and detection from samples including PCR inhibitors. It is also compatible with high-speed PCR cycles.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eFeatures\u003c\/h2\u003e\n\u003cul style=\"list-style-type: none; padding-left: 0;\"\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e \u003cstrong\u003eBroad dynamic range\u003c\/strong\u003e\u003cbr\u003eHigh efficiency and specific amplification enable analysis over a broad dynamic range.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e \u003cstrong\u003eFast cycles with 10 s extension times achievable\u003c\/strong\u003e\u003cbr\u003eTargets can be detected even in fast PCR with an extension time of 10s.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e \u003cstrong\u003eImproved quantitative performance in the presence of PCR inhibitors\u003c\/strong\u003e\u003cbr\u003eEfficient amplification can be performed even in the presence of PCR inhibitors.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e \u003cstrong\u003eEnhanced multiplex detection performance\u003c\/strong\u003e\u003cbr\u003eEfficient multiplex detection amplification can be performed.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e\u003cstrong\u003e Enhanced stability of prepared reaction mixtures\u003c\/strong\u003e\u003cbr\u003eOur unique Buffer composition improves the stability of primer-template mixtures.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e \u003cstrong\u003eCompatibility with dUTP carryover prevention\u003c\/strong\u003e\u003cbr\u003eThe use of dUTP prevents false positives due to carryover contamination*.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e \u003cstrong\u003eCompatibility with various real-time thermocyclers and reverse transcription reagents\u003c\/strong\u003e\u003cbr\u003eThis kit can be used with typical real-time PCR instruments, and can be combined with various RT reagents. This kit combined with Toyobo's RT reagents (ReverTra Ace™ series and SuperPrep™ series) has been shown to enable highly accurate and sensitive expression analysis.\u003cbr\u003e\u003cbr\u003e* Uracil-DNA Glycosylase (UNG) is not supplied with this kit. Uracil-DNA Glycosylase (UNG), Heat-labile [Code No.UNG-101] , sold separately, is required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eSpecification\u003c\/h2\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 600px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eCategory\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eItem\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; font-weight: bold;\"\u003eStorage condition\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: center;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eStore at -20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eComponents\u003c\/h2\u003e\n\u003cp\u003eThis kit includes the following components for 100 reactions (QPX-101T) and 500 reactions (QPX-101), with a total of 20μL per reaction. All reagents should be stored at -20°C.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"background: #003366; color: #fff;\"\u003e\n\u003cth style=\"padding: 10px;\"\u003eQPX-101T\u003c\/th\u003e\n\u003cth style=\"padding: 10px;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px;\"\u003eTHUNDERBIRD™ Next probe qPCR Mix\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003e1mL × 1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px;\"\u003e50× ROX reference dye\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003e50μL × 1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"overflow-x: auto;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"background: #003366; color: #fff;\"\u003e\n\u003cth style=\"padding: 10px;\"\u003eQPX-101\u003c\/th\u003e\n\u003cth style=\"padding: 10px;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px;\"\u003eTHUNDERBIRD™ Next probe qPCR Mix\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003e1.67mL × 3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px;\"\u003e50× ROX reference dye\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003e250μL × 1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eApplication Data\u003c\/h2\u003e\n\u003cdiv style=\"margin-bottom: 30px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3\u003eExample 1.Quantification of GAPDH cDNA (total RNA 0.2 pg ~ 20 ng equivalent)\u003c\/h3\u003e\n\u003cp\u003eGAPDH was detected using a fluorescent dye (FAM) labeled TaqMan™ probe.\u003cbr\u003ecDNA from HeLa cell total RNA (total RNA 0.2 pg ~ 20 ng equivalent) was used.\u003cbr\u003eAnalyses confirmed high quantitative performance over a broad dynamic range from 0.2 pg to 20 ng.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_r-pcr-015_01.png?v=1775524475\" style=\"max-width: 100%;\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3\u003eExample 2.Comparison of normal\/fast cycles in multiplex detection system\u003c\/h3\u003e\n\u003cp\u003eβ-actin, GAPDH, PLA, and TUB genes were detected using a multiplex detection system with TaqMan™ probes labeled with four different fluorescent dyes in a \"Normal cycle\" with an extension time of 30 s and in a \"Fast cycle\" with an extension time of 10s.\u003cbr\u003e4-fold dilutions [5 steps] of cDNA derived from HeLa cell total RNA were used as samples and analyzed using Bio-Rad CFX96.\u003cbr\u003eHigh PCR efficiency was demonstrated in fast cycles, even in multiplex detection system where amplification is difficult.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_r-pcr-015_02.png?v=1775524475\" style=\"max-width: 100%;\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3\u003eExample 3.Evaluation of multiplex performance in the presence of inhibitors (EtOH, hematin, heparin, humic acid, UTM, EDTA)\u003c\/h3\u003e\n\u003cp\u003eβ-actin, GAPDH, PLA, and TUB genes were detected using a multiplex detection system with TaqMan™ probes labeled with four different fluorescent dyes in the presence of the inhibitors described in the figure below.\u003cbr\u003e4-fold dilutions [5 steps] of cDNA from HeLa cell total RNA were used as samples and analyzed using Bio-Rad’s CFX96.\u003cbr\u003eAddition of PCR inhibitors caused amplification failure with other companies' reagents. By contrast, using THUNDERBIRD™ Next Probe qPCR Mix, no significant difference in efficiency was observed even when inhibitors were added.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_r-pcr-015_03.png?v=1775524475\" style=\"max-width: 100%;\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3\u003eExample 4.Evaluation of multiplex performance in the presence of inhibitors (feces, whole blood, saliva)\u003c\/h3\u003e\n\u003cp\u003egDNA derived from Shigella, VTEC, and Salmonella were detected using a multiplex detection system with TaqMan™ probes labeled with four different fluorescent dyes. These targets were detected in the presence of PCR inhibitors (feces, whole blood, and saliva). These detections were performed using Bio-Rad's CFX96.\u003cbr\u003eWhile other companies' reagents cannot efficiently amplify in a multiplex detection system in the presence of PCR inhibitors, THUNDERBIRD™ Next Probe qPCR Mix can detect as low as 10 copies of each bacterial gDNA even in the presence of PCR inhibitors.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_r-pcr-015_04.png?v=1775524558\" style=\"max-width: 100%;\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3\u003eExample 5.Stability of prepared reaction mixtures (including primers, probes, and templates)\u003c\/h3\u003e\n\u003cp\u003eAfter mixing primers, probes, and templates in PCR mixtures, target amplification was performed immediately or after incubation for 48 hours at room temperature, protected from light.\u003cbr\u003eCt value delayed after 48 hours with other companies' products; however, THUNDERBIRD™ Next Probe qPCR Mix showed stable performance even after 48 hours.\u003cbr\u003eIf there is a difference in ΔCt of 0.5 or more, or if ΔCt cannot be detected, the column is highlighted in yellow.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_r-pcr-015_05.png?v=1775524558\" style=\"max-width: 100%;\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDocuments\u003c\/h2\u003e\n  \u003cdiv style=\"border: 1px solid #eee; background-color: #f9f9f9; padding: 24px; border-radius: 8px;\"\u003e\n\u003cdiv style=\"display: flex; flex-wrap: wrap; gap: 15px;\"\u003e\n\u003ca style=\"display: inline-block; background-color: #003366; color: #ffffff; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold;\" rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/QPX-101_manual_EN.pdf?v=1775800877\" target=\"_blank\"\u003e Download Manual \u003c\/a\u003e \n  \u003ca style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/QPX-101_SDS.pdf?v=1775800877\" target=\"_blank\"\u003e Download SDS \u003c\/a\u003e\n  \u003ca style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/QPX-101_Eng.pdf?v=1775800993\" target=\"_blank\"\u003e Download Flyer \u003c\/a\u003e\n\u003ca style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" rel=\"noopener noreferrer\" href=\"https:\/\/biofargo.com\/pages\/contact-us?srsltid=AfmBOorrYySVXlrC6mOen85FBiTKooBmPEyrEjjsqXyC0kKPRm7fDFPV\" target=\"_blank\"\u003e Request COA \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n","brand":"Toyobo","offers":[{"title":"100Rxn","offer_id":52417309638837,"sku":"QPX-101T","price":28.8,"currency_code":"USD","in_stock":true},{"title":"500Rxn","offer_id":52417309671605,"sku":"QPX-101","price":189.6,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/THUNDERBIRD_Next_Probe_qPCR_Mix-Toyobo.jpg?v=1777517198","url":"https:\/\/biofargo.com\/products\/thunderbird-next-probe-qpcr-mix","provider":"Biofargo","version":"1.0","type":"link"}