{"product_id":"thunderbird-next-probe-one-step-qrt-pcr-4x-mix","title":"THUNDERBIRD Next Probe One-step qRT-PCR 4x Mix-Toyobo","description":"\u003cdiv style=\"font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif; color: #333; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 10px;\"\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDescription\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #f9f9f9; padding: 20px; border-radius: 4px; border: 1px solid #eee;\"\u003e\n\u003cp style=\"margin-top: 0;\"\u003eTHUNDERBIRDTMNext Probe One-step qRT-PCR 4×Mix is a one-step real-time reverse-transcription polymerase chain reaction (RT-PCR) mix that uses the highly efficient reverse transcriptase “ReverTra AceTM” and Hot Start TTx DNA Polymerase. The combination of these two enzymes and our optimized buffer system enables the quickly and highly sensitively detection and quantification of small quantities of RNA. This mix does not contain glycerol and contains a lyophilization stabilizer; therefore, there should be no need to add any further excipients to assist lyophilization. After freeze-drying, it can be stored stably at room temperature and transported without the need for ice packs. In addition, real-time PCR reactions can be performed immediately by simply adding a sample containing a template and water. Freeze-drying does not impact the performance of this PCR mix.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eFeatures\u003c\/h2\u003e\n\u003cul style=\"list-style-type: none; padding-left: 0;\"\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e \u003cstrong\u003eRapid and Sensitive\u003c\/strong\u003e\u003cbr\u003eThis corresponds to a fast cycle.\u003cbr\u003eIt enables rapid and sensitive detection of trace amounts of RNA.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e \u003cstrong\u003eEasy to use\u003c\/strong\u003e\u003cbr\u003eThe primers, probes, and samples are simply added to initiate the reaction.\u003cbr\u003eThis reduces the risk of contamination and pipetting errors.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e \u003cstrong\u003eImproving contaminant resistance\u003c\/strong\u003e\u003cbr\u003eEfficient amplification can be achieved even in the presence of contaminants that inhibit PCR.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e \u003cstrong\u003eHigh multiplex performance\u003c\/strong\u003e\u003cbr\u003eThis corresponds to the multiplex reactions that can simultaneously detect multiple genes.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e \u003cstrong\u003eCompatibility with dUTP carryover prevention (combined with UNG sold separately)\u003c\/strong\u003e\u003cbr\u003eThe use of dUTP prevents false positives due to carryover contamination.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e \u003cstrong\u003eEnables lyophilization\u003c\/strong\u003e\u003cbr\u003eExcipients for lyophilization are included, allowing for the preparation of lyophilized reagents.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eSpecification\u003c\/h2\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 600px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eCategory\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eItem\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; font-weight: bold; background-color: #fcfcfc;\"\u003eStorage condition\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: center;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eStore at -20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003eThis kit includes the following components for 250 reactions (QRX-101), with a total of 20μL per reaction. All reagents should be stored at -20°C.\u003c\/p\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eComponents\u003c\/h2\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 600px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eQRX-101\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eTHUNDERBIRD™ Next Probe One-step qRT-PCR 4×Mix\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e1.25mL × 1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e50× ROX reference dye\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e100μL × 1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e* Uracil-DNA Glycosylase (UNG) is not supplied with this kit.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eApplication Data\u003c\/h2\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 1.Detection sensitivity comparison\u003c\/h3\u003e\n\u003cp\u003eUsing TaqManTM probe, we compared the detection sensitivity and quantitation of mumps virus RNA with other companies' products.\u003cbr\u003eOnly this kit was able to detect RNA of less than 10 copies and quantify it over a wide dynamic range. This kit is effective in detecting RNA viruses and mRNAs with low expression levels.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/application1.jpg?v=1775530200\" alt=\"\" style=\"max-width: 100%; height: auto;\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 2.Detection comparison during fast cycles\u003c\/h3\u003e\n\u003cp\u003eInfluenza virus RNA was detected using the “Normal” cycle with an elongation time of 45 seconds and the “Fast” cycle with an elongation time of 10 seconds. Analysis was performed using Applied BiosystemsTM StepOnePlusTM.\u003cbr\u003eAs a result, efficient amplification was possible even with a fast cycle of 10 s elongation time.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Application2_0.jpg?v=1775530200\" alt=\"\" style=\"max-width: 100%; height: auto;\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 3.Comparison of the detection sensitivitiesfor different viruses\u003c\/h3\u003e\n\u003cp\u003eThe detection sensitivity of five types of viral RNA were cmpared using TaqManTM probes.\u003cbr\u003eApplied BiosystemsTM StepOnePlusTM was used for the analysis.\u003cbr\u003eThis product was able to detect all targets with a sensitivity of 10 copies or less.\u003cbr\u003eIt is possible to detect various RNAs with high sensitivity, regardless of the primer\/probe sequence.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Application3_0.jpg?v=1775530200\" alt=\"\" style=\"max-width: 100%; height: auto;\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 4.Tolerant of contaminant\u003c\/h3\u003e\n\u003cp\u003eBiological samples (nasal swabs, saliva, and plasma) were added to the reaction to detect 50 copies of influenza virus RNA and evaluate resistance to PCR inhibitors. Bio-Rad CFX96 Touch Deep Well was used for the analysis.\u003cbr\u003eWhile other company reagents caused poor amplification when PCR inhibitors were added, this reagent showed minimal effect on Ct even when biological samples were added.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Application4_0.jpg?v=1775530200\" alt=\"\" style=\"max-width: 100%; height: auto;\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 5.Comparison between singleplex and multiplex methods\u003c\/h3\u003e\n\u003cp\u003eSingle-and multiplex detections were compared using TaqManTM probes labeled with four different fluorescent dyes. HeLa S3 RNA (10x dilutions of 500 ng to 0.05 ng [5 steps]) was used as a template.\u003cbr\u003eThe results revealed that single and multiplex detection showed comparable PCR efficiencies and that multiplex detection did not affect the reaction.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Application5_0.jpg?v=1775530200\" alt=\"\" style=\"max-width: 100%; height: auto;\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 6.Carryover prevention by adding UNG\u003c\/h3\u003e\n\u003cp\u003eAssuming that the splashed amplified product was contaminated in the subsequent PCR (carryover contamination), we used the amplified sample (196bp) equivalent to 103, 102 copies as a template and detected the same target under the conditions of UNG addition (+)\/(-).\u003cbr\u003eAnalysis was performed using Applied BiosystemsTM StepOnePlusTM.\u003cbr\u003eAs a result, the template was degraded and no amplification was observed when UNG was added.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/application6_0.jpg?v=1775530200\" alt=\"\" style=\"max-width: 100%; height: auto;\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 7.Performance comparison before and after lyophilization\u003c\/h3\u003e\n\u003cp\u003eqPCR Mix was prepared using THUNDERBIRDTM Next Probe One-step qRT-PCR 4×Mix, and 1500, 375, 94, 23, and 6 copies of influenza A virus RNA were detected before and after lyophilization, respectively. Analysis was performed using a Bio-Rad CFX96 Touch Deep Well.\u003cbr\u003eGood amplification was obtained in all cases and no freeze-drying effects were observed.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/application7_0.jpg?v=1775530200\" alt=\"\" style=\"max-width: 100%; height: auto;\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDocuments\u003c\/h2\u003e\n\u003cdiv style=\"border: 1px solid #eee; background-color: #f9f9f9; padding: 24px; border-radius: 8px;\"\u003e\n\u003cdiv style=\"display: flex; flex-wrap: wrap; gap: 15px;\"\u003e\n\u003ca style=\"display: inline-block; background-color: #003366; color: #ffffff; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold;\" rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Manual_QRX-101_2407.pdf?v=1775802491\" target=\"_blank\"\u003e Download Manual \u003c\/a\u003e \n  \u003ca style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/SDS_QRX-101_2407.pdf?v=1775802487\" target=\"_blank\"\u003e Download SDS \u003c\/a\u003e\n  \u003ca style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/QRX-101_Eng.pdf?v=1775802533\" target=\"_blank\"\u003e Download Flyer \u003c\/a\u003e\n\u003ca style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" rel=\"noopener noreferrer\" href=\"https:\/\/biofargo.com\/pages\/contact-us?srsltid=AfmBOorrYySVXlrC6mOen85FBiTKooBmPEyrEjjsqXyC0kKPRm7fDFPV\" target=\"_blank\"\u003e Request COA \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Toyobo","offers":[{"title":"250Rxn","offer_id":52417379664053,"sku":"QRX-101","price":280.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/THUNDERBIRD_Next_Probe_One-step_qRT-PCR_4x_Mix-Toyobo.jpg?v=1777517859","url":"https:\/\/biofargo.com\/products\/thunderbird-next-probe-one-step-qrt-pcr-4x-mix","provider":"Biofargo","version":"1.0","type":"link"}