{"product_id":"three-color-prestained-protein-marker-10-250-kda","title":"Three-color Prestained Protein Marker (10–250 kDa)","description":"\u003c!-- =========================\nPRODUCT DESCRIPTION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eThree-color Prestained Protein Marker (10–250 kDa) is a ready-to-use protein molecular weight marker designed for accurate protein size estimation during SDS-PAGE and Western blot workflows. During protein electrophoresis experiments, unclear band positioning, inconsistent molecular weight estimation, and difficulty monitoring membrane transfer efficiency can affect downstream protein analysis. A reliable prestained protein ladder provides a visible reference standard to help researchers evaluate protein migration, gel separation performance, and transfer results.\u003c\/p\u003e\n\u003cp\u003eThis three-color protein marker contains 10 prestained protein bands covering a broad molecular weight range from 10 kDa to 250 kDa. The color-coded design includes a green 10 kDa reference band, an orange 70 kDa reference band, and blue reference bands at other molecular weights, enabling rapid visualization of protein separation during electrophoresis. The marker can be directly loaded onto polyacrylamide gels without additional staining procedures and is suitable as a protein molecular weight marker for routine laboratory workflows.\u003c\/p\u003e\n\u003cp\u003eCompared with traditional unstained protein standards, this prestained protein ladder allows researchers to monitor protein migration in real time during SDS-PAGE and provides a convenient reference for Western blot membrane transfer evaluation. Three-color Prestained Protein Marker (10–250 kDa) is suitable for protein expression analysis, recombinant protein characterization, cell lysate analysis, and general protein electrophoresis applications.\u003c\/p\u003e\n\u003cp\u003eThis protein marker is supplied in a ready-to-use format and requires no dilution or heating before loading, making it an efficient solution for laboratories performing frequent SDS-PAGE and Western blot experiments.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nSPECIFICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eThree-color Prestained Protein Marker (10–250 kDa)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eRP0021S\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eVolume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e250 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003ePrestained Protein Molecular Weight Marker\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eMolecular Weight Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e10–250 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eNumber of Bands\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e10 Prestained Protein Bands\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eColor System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eThree-color design (Green, Orange, Blue)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eReference Bands\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e10 kDa Green Band; 70 kDa Orange Band\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eRecommended Loading Volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e5 μL per lane\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eSDS-PAGE, Western Blot, Protein Electrophoresis, Protein Size Estimation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eAt least 2 years\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFEATURES\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eThree-color visual identification:\u003c\/strong\u003e Color-coded protein bands provide rapid visualization of molecular weight positions during SDS-PAGE and Western blot workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBroad molecular weight coverage:\u003c\/strong\u003e Covers a range of 10–250 kDa, supporting analysis of a wide variety of proteins from small proteins to high molecular weight targets.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eReady-to-use protein ladder:\u003c\/strong\u003e Supplied in a convenient format that can be directly loaded after thawing and mixing, reducing preparation steps before electrophoresis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eReliable reference for Western blot:\u003c\/strong\u003e Helps researchers evaluate protein transfer efficiency and identify membrane transfer performance during blotting workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eStable performance:\u003c\/strong\u003e Maintains product stability under recommended storage conditions for consistent use in routine protein analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCompatible with common protein electrophoresis workflows:\u003c\/strong\u003e Suitable for SDS-PAGE gels and standard Western blot procedures used in molecular biology laboratories.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nAPPLICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThree-color Prestained Protein Marker (10–250 kDa) is widely used as a protein electrophoresis standard in molecular biology, biochemistry, and life science research laboratories. The visible reference bands provide convenient support for protein separation analysis and Western blot workflows.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eWestern Blot Protein Analysis:\u003c\/strong\u003e Used as a Western blot marker to estimate target protein molecular weight and monitor protein transfer from gel to membrane.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSDS-PAGE Protein Electrophoresis:\u003c\/strong\u003e Provides visible molecular weight references for evaluating protein migration and electrophoretic separation performance.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein Molecular Weight Estimation:\u003c\/strong\u003e Enables approximate determination of target protein size by comparing sample bands with known protein standards.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCell Lysate Analysis:\u003c\/strong\u003e Supports protein expression analysis from whole-cell lysates prepared from cultured cells.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Protein Characterization:\u003c\/strong\u003e Used during recombinant protein expression and purification workflows to evaluate protein size and purity.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein Transfer Monitoring:\u003c\/strong\u003e Helps researchers visually confirm transfer efficiency during Western blot membrane preparation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein Electrophoresis Quality Control:\u003c\/strong\u003e Provides a consistent molecular weight reference for routine laboratory protein analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eMolecular Biology Research:\u003c\/strong\u003e Suitable for general protein detection workflows involving SDS-PAGE, immunoblotting, and biochemical studies.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPROTOCOL OVERVIEW\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eProtocol Overview\u003c\/h2\u003e\n\u003cp\u003eThree-color Prestained Protein Marker (10–250 kDa) is supplied as a ready-to-use protein ladder for SDS-PAGE and Western blot applications. Proper handling before electrophoresis helps maintain clear band appearance and consistent migration performance.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eAllow the protein marker to equilibrate to room temperature before use.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMix gently by vortexing or pipetting to ensure uniform distribution of prestained protein bands.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eLoad approximately 5 μL of the protein molecular weight marker per lane for routine SDS-PAGE analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003ePerform electrophoresis using an appropriate polyacrylamide gel concentration according to the expected molecular weight range of target proteins.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFor low molecular weight proteins below 25 kDa, higher percentage separation gels (≥15%) are recommended to improve band resolution.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFor Western blot workflows, optimize transfer conditions according to protein size. High molecular weight proteins may require extended transfer time to achieve efficient membrane transfer.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eBecause prestained protein markers contain dyes covalently associated with proteins, their electrophoretic migration may differ slightly from unstained protein standards. Therefore, this protein ladder should be used for approximate molecular weight estimation rather than precise molecular mass determination.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nORDERING INFORMATION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eOrdering Information\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eRP0021S\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eThree-color Prestained Protein Marker (10–250 kDa)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e250 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFAQ\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is a three-color prestained protein marker used for?\u003c\/summary\u003e\n\u003cp\u003eA three-color prestained protein marker is used as a visible molecular weight reference during SDS-PAGE and Western blot experiments. It helps researchers estimate protein size, monitor electrophoresis separation, and evaluate membrane transfer performance.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the molecular weight range of this protein ladder?\u003c\/summary\u003e\n\u003cp\u003eThis prestained protein ladder covers a molecular weight range of 10–250 kDa and contains 10 colored protein bands suitable for analysis of a wide variety of proteins.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan this prestained protein marker be used for Western blot?\u003c\/summary\u003e\n\u003cp\u003eYes. Three-color Prestained Protein Marker (10–250 kDa) is suitable as a Western blot marker for monitoring protein transfer efficiency and estimating the molecular weight position of target proteins after membrane transfer.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the difference between prestained and unstained protein markers?\u003c\/summary\u003e\n\u003cp\u003ePrestained protein markers contain color labels that allow direct visualization during electrophoresis and transfer workflows, while unstained protein markers require additional staining procedures for visualization. Prestained markers provide faster workflow monitoring but are mainly used for approximate molecular weight estimation because dye modification can affect protein migration.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow much protein marker should be loaded for SDS-PAGE?\u003c\/summary\u003e\n\u003cp\u003eApproximately 5 μL of Three-color Prestained Protein Marker (10–250 kDa) is recommended for routine SDS-PAGE analysis. The optimal loading volume may vary depending on gel thickness, staining conditions, and detection requirements.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhy are low molecular weight bands difficult to separate on some gels?\u003c\/summary\u003e\n\u003cp\u003eProtein marker resolution depends on polyacrylamide gel concentration. When separation gels with concentrations below 15% are used, proteins below 25 kDa may not separate clearly. Higher percentage gels are recommended for improved resolution of small molecular weight proteins.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan prestained protein markers accurately determine protein molecular weight?\u003c\/summary\u003e\n\u003cp\u003ePrestained protein markers provide approximate molecular weight estimation. Since protein dyes can influence electrophoretic mobility, precise molecular weight determination is better performed using appropriate unstained protein standards.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow should this protein molecular weight marker be stored?\u003c\/summary\u003e\n\u003cp\u003eStore the protein marker at -20 °C. Under recommended storage conditions, the product remains stable for at least two years.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPRODUCT SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!-- =========================\nFAQ SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AccuRef Scientific","offers":[{"title":"250μL","offer_id":52776611578037,"sku":"RP0021S","price":95.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_1027008c-eb3b-4357-a825-f59ac422e85a.jpg?v=1783499607","url":"https:\/\/biofargo.com\/products\/three-color-prestained-protein-marker-10-250-kda","provider":"Biofargo","version":"1.0","type":"link"}