Description

This product adopts magnetic beads and a unique buffer system to isolate and purify high-quality genomic DNA from various plant tissues. The uniquely embedded magnetic beads have a strong affinity for nucleic acid under certain conditions. When the conditions are changed, the magnetic beads can release the absorbed nucleic acid to rapidly separate and purify the nucleic acid.

It can be perfectly fitted with the TGuide S16 Nucleic Acid Extractor. Through absorption, transfer, and release of magnetic beads by the special magnetic bar, magnetic beads and nucleic acid can be transferred to improve the degree of automation. The whole process is safe and convenient, and the extracted genomic DNA fragments are large, with high purity and reliable quality.

The DNA purified by this kit is suitable for a range of common downstream applications, including digestion, PCR, library construction, Southern hybridization, and other experiments.

Features

Simple and fast: Ultra-pure genomic DNA can be obtained by running TGuide S16 for 60 minutes.
Widely applicable: Suitable for a variety of plant tissues, especially polysaccharide- or polyphenol-rich plants.
Safe and non-toxic: No toxic organic reagents such as phenol/chloroform.
High purity: The obtained DNA has high purity and can be directly used for chip detection, high-throughput sequencing, and other experiments.

Kit Contents

Components		Quantity
GDP607-DH	Buffer GPS	30 ml
	Buffer GPA	10 ml
	Plant DNA Reagents	48
	Proteinase K	1 ml
	RNase A (100 mg/ml)	200 μl
	Buffer TB	15 ml
OSE-TGA-S36	TGuide Smart Tip Comb	12 pcs

Applications

For purification of genomic DNA from various plant Tissues.

Storage Condition

All components of the kit can be stored in dry conditions at room temperature (15~30°C) for 12 months. If the solution precipitates, it can be preheated in a water bath at 37°C for 10 min before use to dissolve the precipitation, without affecting the effect.

Important Notes

To use the TGuide Smart Magnetic Plant DNA Kit, you must have the TGuide Smart Magnetic Plant DNA (program no. DP607) installed on the TGuide S16/S32 pro Nucleic Acid Extractor.
Repeated freezing and thawing samples should be avoided, otherwise the extracted DNA fragments will be small and the total yield will decrease.
If there is precipitation in the buffer GPS, it can be dissolved in a 37°C water bath and used after shaking well.

Operational Steps

Detection of DNA Concentration and Purity

The size of the obtained genomic DNA fragment is affected by the sample storage time and shear force during operation. The concentration and purity of the obtained DNA fragments can be detected by agarose gel electrophoresis and UV spectrophotometer. Ideally, the DNA should absorb at most at OD260, where an OD260 value of 1 corresponds to approximately 50 μg/ml double strand DNA and 40 μg/ml single strand DNA. The OD260/OD280 ratio should be 1.7~1.9.

Recommended Usage

This kit is designed for both automated and manual nucleic acid extraction workflows.

Automated use: For optimal performance, the TGuide Smart Magnetic Plant DNA Kit should be used with the TGuide S16 Nucleic Acid Extractor, ensuring fully automated and standardized purification.
Manual use: When operated manually, a magnetic rack is required to complete the magnetic bead separation and washing steps.

Documents

Manual

TGuide Smart Magnetic Plant DNA Kit-Tiangen