Description

This kit uses a unique lysis solution combined with a specially optimized and highly efficient HA removal buffer to extract genomic DNA of Gram-negative bacteria, Gram-positive bacteria, fungi and other microorganisms from a variety of environmental samples, such as soil, feces, fermentation products, water, pond sludge, marine sediments, and intestinal contents. The grinding beads and homogenizer can effectively crush the microorganisms in the environmental samples to ensure the high efficiency and integrity of the extracted genomic DNA. Genomic DNA purified by this kit has few impurities and good integrity, and can be directly used in downstream experiments of molecular biology such as PCR, library prep and NGS.

Kit Contents

Contents		GDP613-DE (48 preps)
GDP613-DH	Buffer SNA	40 ml
	HA Removal Buffer CN	15 ml
	RNase A (10 mg/ml)	500 µl
	Buffer TB	15 ml
	Buffer TE	15 ml
	Envir-DNA Reagents	48 tubes
	Envir-DNA Grinding Beads Tube	48 tubes/pack
OSE-TGA-S36	TGuide Smart Tip Comb	12 pcs

Features

Quick and easy: Quickly remove humic acid in 1 min.
High purity: Combined with magnetic bead purification, the extracted DNA has high purity and can be directly used in downstream experiments.
Wide application: Can be applied to microbial genomic DNA extraction for many types of samples.
Good compatibility: It can be applied to a wide range of TGuide S16/S32/S32 Pro automated nucleic acid extractor and can be extended for use with the TGuide S96 Dex Automated Nucleic Acid Extractor.

Applications

For purification DNA from various environmental samples such as soil, stool, fermentation products, water, pond sludge, marine sediment, and intestinal contents, etc.

Storage

The kit can be stored for 12 months under dry conditions at room temperature (15-30℃); if the solution produces precipitation, it can be preheated in a 37℃ water bath for 10 min to dissolve the precipitate.

Notes

The newly collected samples will get higher yield, and different samples should consult the corresponding optimal preservation conditions before sampling.
Excess DNA may inhibit downstream PCR reactions, and it is recommended to dilute the DNA template for use if inhibition appeared.
HA Removal Buffer CN needs to be pre-cooled at 2-8°C before use in order to achieve its optimal humic acid removal effect.
Buffer TB does not contain EDTA. In order to minimize the degradation of the extracted nucleic acids for long time storage, customer can replace the Buffer TB with the EDTA-containing Buffer TE if necessary, but pay attention to the fact that the EDTA will affect the downstream experiments, such as PCR, library construction, and so on.
Due to the complexity of environmental samples, which usually have more impurities, the concentration of extracted nucleic acids is low, and degradation of nucleic acids may occur in long time storage, etc. It is recommended to carry out downstream experiments as soon as possible after extraction.
This kit is equipped with two elution buffers. Choose corresponding one for the blank control.

Documents

INFO

TGuide Smart Envir-DNA Kit (Prefilled Single Sample Cartridge)