Description

Cas13a is an RNA-guided RNA endonuclease belonging to the class 2 type VI CRISPR-Cas system (1). It possesses both RNA recognition and cleavage activities (2). Activated Cas13a also induces collateral cleavage of nearby non-targeted RNAs in a nonspecific manner (3). This property of the enzyme has been leveraged for the development of robust nucleic acid detection tools with applications in basic research and therapeutics development (4). TccCas13a is thermostable and exhibits robust cis and trans catalytic activities over a broad range of temperatures from 37°C to 70°C (5).

Accession Number

WP_149678719.1

Formulation

Recombinant protein stored in 50mM sodium phosphate pH 7.5, 150mM imidazole, 1mM DTT, 10% glycerol.

Storage

Store product at â€“70oC. For optimal performance aliquot product into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated handling and multiple freeze/thaw cycles. Always include RNase inhibitor at manufacturer recommended concentrations in Cas enzyme reactions.

Documents

TccCas13a, Active

References

East-Seletsky A, et al. 2016. Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection. Nature. 538:270â€“3. 2.
Abudayyeh O, et al. 2016. C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector. Science. 353:5573 3.
Zhang J, You Y. 2020. CRISPR-Cas13a system: A novel approach to precision oncology. Cancer Biol Med. 17(1):6-8.
Patchsung M, et al. 2020. Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA. Nat Biomed Eng. 4(12):1140-1149.
Mahas A, et al. 2022. Characterization of a thermostable Cas13 enzyme for one-pot detection of SARS-CoV-2. Proc Natl Acad Sci U S A. 119(28):e2118260119.