Description

This product was purified from a plasmid cloned with a gene encoding Thermus aquaticus DNA polymerase and induced expression by E. coli. Taq DNA polymerase does not have 3'→5' exonuclease correction activity, but has very low 5'→3' exonuclease activity. Recombinant Taq DNA polymerase is the recommended choice for most PCR reactions, with a half-life of > 40 min at 95°C. The mutation rate of each circulating Taq DNA polymerase < 2.2×10-5. The above-mentioned Taq DNA polymerase is free of contamination by foreign nucleases and bacterial DNA, and has high amplification efficiency and good stability, making it suitable for routine PCR amplification.

Specification

Parameter	Specification
Package	1 KU/5 KU/200 U
Full name	Taq DNA Polymerase
Composition	Taq DNA polymerase (5 U/ul), 10X PCR Buffer without Mgcl2, 25 mM MgCl2 .
Reaction Buffer	10X PCR Buffer
Unit Definition	One unit is defined as the amount of enzyme that catalyzes the incorporation of 10 nmol deoxyribonucleotides into a polynucleotide fraction in 30 min at 74°C.
Concentration	5 U/μl
Storage Buffer	20 mM Tris-HCl (PH 8.0) ；0.1 mM EDTA,；1 mM DTT；0.5% Tween 20；100 mM KCl；0.5% NP-40；50% glycerin.
Component	Taq DNA polymerase (5 U/ul), 10X PCR Buffer without Mgcl2, 25 mM MgCl2 .

Features

There is no contamination of foreign nuclease and bacterial DNA, and the amplification efficiency is high and the stability is good.

Applications

Conventional PCR amplification, RT-PCR, and direct TA cloning of the product.

Storage

-20°C

Taq DNA Polymerase