Synonym(s): 2-Hydroxy-3-[tris(hydroxymethyl)methylamino]-1-propanesulfonic acid, N-[Tris(hydroxymethyl)methyl]-3-amino-2-hydroxypropanesulfonic acid

Description

TAPSO (2-Hydroxy-3-[tris(hydroxymethyl)methylamino]-1-propanesulfonic acid) is a high-purity (≥ 99.0%) biochemical-grade buffering reagent supplied as a white crystalline powder. Its molecular formula is C₇H₁₇NO₇S (molecular weight 259.28) and CAS number 68399‑81‑5. This compound dissolves readily in water to yield a clear, colorless solution — ideal for preparing stable, pH‑controlled buffers. When properly dissolved and titrated (e.g., with NaOH), TAPSO serves as an excellent zwitterionic buffer for biological and biochemical experiments.

Specifications

Item	Value
Chemical Name	3-[[Tris(Hydroxymethyl)Methylamino]-2-Hydroxypropane-Sulfonic Acid
Appearance	White Crystalline Powder
CAS No.	68399-81-5
Molecular Formula	C7H17NO7S
Molecular Weight	259.28
Grade	Biochemical Grade
Purity (as Agreed)	≥99.0%
Moisture	≤1.0%
Solubility (0.1M Aqueous Solution)	Clear Colorless Solution
pH (1% Di H2O)	4.7–5.3
Infrared Spectrum	Complies with Specifications
A260 (in 1M Water)	≤0.040
A280 (in 1M Water)	≤0.020

Features

High Purity: ≥99.0% biochemical grade ensures minimal impurities for sensitive experiments.
Stable pH Buffering: Strong buffering capacity in the pH range of 7.0–8.2.
Excellent Solubility: Readily dissolves in water, producing clear, particle-free solutions.
Low Optical Absorbance: Minimal interference in assays involving proteins or nucleic acids.
Versatile Applications: Suitable for protein chemistry, enzyme assays, and molecular biology workflows.

Applications

Buffer preparation for protein purification, enzyme reactions, and biochemical assays.
Cell culture media formulation requiring stable physiological pH.
Molecular biology protocols where consistent pH and low background absorbance are critical.
General laboratory buffer systems for reproducible results in research experiments.

Safety Disclaimer

This product is intended for laboratory research use only. Not for drug, human, or veterinary use. Review the Safety Data Sheet (SDS) provided by BioFargo for detailed handling, storage, hazard, and disposal information. Use appropriate personal protective equipment (PPE) including gloves, lab coat, and eye protection when handling. Dispose of waste and residual buffer in accordance with local, state, and federal regulations. Ensure proper ventilation if preparing large volumes or heating solutions.

Documents

MSDS

TAPSO 99% Biochemical Grade