Description

TAE Buffer Premix Powder is an economical and space-efficient solution for laboratories that routinely perform nucleic acid electrophoresis. Each package contains a pre-formulated powder blend that produces 1 liter of 25× TAE stock solution, equivalent to 25 liters of 1× working buffer after dilution, making it ideal for high-throughput research environments and laboratories with frequent electrophoresis requirements.

Designed to eliminate the need for weighing individual buffer components, the premixed formulation helps ensure batch-to-batch consistency while reducing preparation errors. Once dissolved and diluted, the resulting TAE buffer can be used as both gel preparation buffer and running buffer for agarose gel electrophoresis of DNA and RNA samples.

TAE buffer is widely used for PCR product analysis, plasmid verification, genomic DNA separation, cloning workflows, gel extraction procedures, and other molecular biology applications. Compared with TBE buffer, TAE allows double-stranded DNA to migrate more rapidly and is often preferred for preparative electrophoresis and downstream DNA recovery workflows.

Specification

Parameter	Specification
Total Product Size	25 L
Individual Container Size	25 L
Number of Containers	1
Shipping Conditions	RT
UNSPSC Code	41105323
UNSPSC Category	Electrophoresis Buffers
Grade	Biotech
Dangerous Goods (DG)	No
Storage	18–25°C
Sterile	No

Features

Produces Up to 25 Liters of 1× Working Buffer

Each package generates 1 liter of 25× stock solution or up to 25 liters of 1× TAE buffer, making it a cost-effective choice for laboratories with frequent electrophoresis needs.
Pre-Mixed Formula Eliminates Weighing Errors
No need to separately measure Tris, acetate, or EDTA components. The premixed format simplifies preparation and improves reproducibility between buffer batches.
Optimized for Agarose Gel Electrophoresis

Suitable for preparing both agarose gels and running buffers used in routine nucleic acid separation workflows.
Faster Double-Stranded DNA Migration
Compared with TBE and TPE systems, double-stranded DNA typically migrates more rapidly in TAE buffer, improving efficiency for routine gel analysis.
Nuclease-Free Quality
The product has been analyzed for the absence of nucleases, supporting reliable nucleic acid electrophoresis applications.
Space-Saving Powder Format

Powder storage reduces shipping weight, lowers storage requirements, and simplifies inventory management compared with ready-made liquid buffers.
Ideal for High-Volume Laboratories

Well suited for molecular biology laboratories, core facilities, teaching laboratories, biotechnology companies, and research institutions performing routine electrophoresis.

Applications

PCR Product Verification

Analyze amplification products, confirm expected fragment sizes, and evaluate PCR reaction quality through agarose gel electrophoresis.
DNA Agarose Gel Electrophoresis

Routine separation and visualization of plasmid DNA, restriction digests, genomic DNA, and other nucleic acid samples.
Gel Extraction and DNA Recovery

Frequently used in preparative electrophoresis workflows prior to DNA purification, cloning, sequencing, or downstream molecular biology procedures.
Molecular Cloning Workflows

Support plasmid screening, recombinant construct verification, restriction enzyme digestion analysis, and cloning experiments.
RNA Electrophoresis

Suitable for agarose gel electrophoresis applications involving RNA analysis and quality assessment.
Southern Blot Preparation

Can be used during electrophoresis steps preceding Southern blot hybridization workflows.
Academic and Research Laboratories

Ideal for teaching laboratories, university research groups, biotechnology companies, contract research organizations, and shared core facilities requiring large volumes of electrophoresis buffer.

Documents

INFO

COA

SDS

TAE Buffer Premix Powder Biotech Grade