Description

TAE buffer 50x solution: TAE (Tris/Acetate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but dsDNA runs faster with TAE buffer. This 25x and 50x concentrate can be easily diluted with molecular biology grade water to 1x before use.

Specification

Parameter	Specification
Total Product Size	1 L
Individual Container Size	1 L
Number of Containers	1
Shipping Conditions	RT
UNSPSC Code	41105323
UNSPSC Category	Electrophoresis Buffers
Grade	Biotech
Dangerous Goods (DG)	No
Storage	18–25°C
Sterile	No
Stock Status	Usually in Stock

Features

Ready-to-Use 50× Concentrate
Simply dilute with molecular biology grade water to prepare a 1× working solution, reducing preparation time and improving laboratory efficiency.
Optimized for DNA Electrophoresis
Provides reliable conductivity and buffering performance for routine nucleic acid separation and analysis.
Faster dsDNA Migration
Compared with TBE buffer, TAE buffer allows double-stranded DNA fragments to migrate more rapidly through agarose gels, helping reduce run times.
Ideal for DNA Recovery Workflows
Frequently selected for gel extraction, cloning, sequencing preparation, and other applications where DNA recovery after electrophoresis is important.
Space-Saving High-Concentration Format
The 50× concentration reduces storage requirements and supports preparation of large volumes of running buffer from a single bottle.
Consistent Experimental Performance
Manufactured to Biotech Grade standards with controlled pH and composition for reproducible electrophoresis results.

Applications

PCR Product Analysis

Verify amplification success, assess amplicon size, and evaluate PCR specificity through agarose gel electrophoresis.
Plasmid DNA Characterization

Analyze plasmid preparations, restriction digestion products, and cloning constructs during molecular biology workflows.
Genomic DNA Separation

Suitable for evaluating DNA integrity and resolving larger DNA fragments.
DNA Gel Extraction and Purification

Commonly used prior to DNA recovery procedures for cloning, sequencing, mutagenesis, and other downstream applications.
Southern Blot Workflows

Can be used as a running buffer during electrophoresis steps preceding Southern blot hybridization procedures.
Academic and Research Laboratories

Ideal for routine nucleic acid electrophoresis performed in molecular biology, genetics, biotechnology, and life science research laboratories.

Documents

INFO

COA

SDS

TAE Buffer 50x Solution Biotech Grade - Biofargo