Description

TAE Buffer 25× Solution is a concentrated electrophoresis buffer designed for convenient preparation of nucleic acid running buffer in molecular biology laboratories. Simply dilute the solution with molecular biology grade water to obtain a 1× working buffer, eliminating the need for weighing and mixing individual buffer components while helping ensure consistent experimental results.

Widely used for agarose gel electrophoresis, TAE buffer supports the separation and analysis of PCR products, plasmid DNA, genomic DNA, restriction fragments, and other nucleic acid samples. Compared with TBE buffer, TAE allows double-stranded DNA to migrate more rapidly and is often preferred for applications involving larger DNA fragments and downstream DNA recovery procedures. This makes it a popular choice for cloning, sequencing preparation, gel extraction, and routine molecular biology workflows.

The 25× concentrate format offers an ideal balance between convenience and storage efficiency, allowing researchers to prepare large volumes of working buffer while minimizing preparation time and laboratory variability.

Specification

Parameter	Specification
Total Product Size	1 L
Individual Container Size	1 L
Number of Containers	1
Shipping Conditions	Room Temperature (RT)
UNSPSC Code	41105323
UNSPSC Category	Electrophoresis Buffers
Grade	Biotech
Dangerous Goods (DG)	No
Storage	18–25°C
Sterile	No
Stock Status	Usually in Stock

Features

Convenient 25× Concentrated Format

Quickly prepare 1× working buffer by simple dilution with molecular biology grade water, reducing preparation time and minimizing handling errors.
Optimized for DNA Agarose Gel Electrophoresis

Designed for routine nucleic acid separation, analysis, and visualization in molecular biology laboratories.
Faster Double-Stranded DNA Migration

Compared with TBE buffer systems, TAE buffer allows dsDNA fragments to migrate more rapidly through agarose gels, improving efficiency for many routine electrophoresis applications.
Supports Downstream DNA Recovery

Frequently used for gel extraction, cloning, plasmid construction, sequencing preparation, and other workflows where DNA recovery after electrophoresis is important.
Consistent Buffer Composition

Pre-formulated solution helps reduce batch-to-batch variability and improves reproducibility across experiments. The 1× working solution contains Tris, acetate, and EDTA at controlled concentrations.
Ready for High-Frequency Laboratory Use

Suitable for research laboratories, academic institutions, biotechnology companies, and core facilities performing routine electrophoresis experiments.
Room-Temperature Storage

Stable storage at 18–25°C simplifies laboratory inventory management and daily use.

Applications

PCR Product Analysis

Verify amplification success, evaluate product specificity, and confirm expected fragment sizes using agarose gel electrophoresis.
Plasmid DNA Verification
Analyze plasmid preparations, restriction enzyme digestions, and recombinant DNA constructs during cloning workflows.
Genomic DNA Separation
Resolve and evaluate genomic DNA samples, including larger DNA fragments that benefit from TAE-based electrophoresis.
DNA Gel Extraction
Perform preparative electrophoresis prior to DNA purification, cloning, sequencing, mutagenesis, and other downstream molecular biology procedures.
Restriction Fragment Analysis
Separate and visualize DNA fragments generated by restriction enzyme digestion for molecular characterization and construct validation.
Southern Blot Preparation
Suitable for electrophoresis procedures preceding Southern blot hybridization workflows.
Academic and Research Laboratories
Ideal for routine nucleic acid electrophoresis applications in molecular biology, genetics, biotechnology, microbiology, and life science research laboratories.

Documents

INFO

COA

SDS

TAE Buffer 25x Solution Biotech Grade