{"product_id":"t75-cell-culture-flask-with-seal-cap-tc-treated-sterile","title":"T75 Cell Culture Flasks with Vented Cap TC Treated Sterile Biofargo","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eBiofargo cell culture flasks feature flat surfaces that offer a large culture area and promote growth. Vented caps with a 0.2 μm filter promote ventilation and prevent contamination. The angled neck provides fast and easy access. Meticulous manufacturing process guarantees reliability for your cell culture work. Each flask features two large noting areas that allow quick identification. Flasks are available in three different sizes: \u003ca href=\"https:\/\/biofargo.com\/products\/t25-cell-culture-flask-with-vent-cap-tc-treated-steriled?variant=51539735806133\"\u003eT25\u003c\/a\u003e, \u003ca href=\"https:\/\/biofargo.com\/products\/t75-cell-culture-flask-with-seal-cap-tc-treated-steriled?variant=51539739082933\"\u003eT75\u003c\/a\u003e, and \u003ca href=\"https:\/\/biofargo.com\/products\/t175-cell-culture-flask-with-vent-cap-tc-treated-steriled?Angled%20neck%20provides%20fast%20and%20easy%20access.variant=51539749929141\"\u003eT175\u003c\/a\u003e.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse; height: 237px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\"\u003eColour\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eFlask: clear\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eCap: Red\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\"\u003eMaterial\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eFlask: PS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eCap: HDPE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eSterilization\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eIrradiation sterilization\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 50%; text-align: center; vertical-align: middle; height: 39px;\"\u003eVolume\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 21px;\"\u003eGrowth area: 75cm\u003csup\u003e2\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eMax. volume: 245mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eTreatment\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eTC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd rowspan=\"3\" style=\"width: 50%; text-align: center; vertical-align: middle; height: 90px;\"\u003eQuality Control\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eRaw Material-Control: physical testing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 36px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\"\u003eProduct-Control: testing of attributive and variable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 36px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\"\u003echaracteristics in accordance with the valid specification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eTC treated\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eVented caps with \u003cspan\u003e0.2 μm filter, promoting ventilation and preventing contamination.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eAngled bottle neck designed for pipettes and cell scrapers.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eMeasurements and annotation area on both sides.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eSterilized self-sealing bag to prevent contamination.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eEasily stackable.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eGamma sterilized, DNase-free, RNase-free.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eTC-Treated (Adherent Treatment):\u003c\/p\u003e\n\u003cp\u003eScenario: Suitable for the culture of adherent cells, providing excellent cell adhesion.\u003c\/p\u003e\n\u003cp\u003eEffect: TC-treated cell culture flasks offer strong adhesion properties, making them ideal for long-term cell culture and large-scale cell expansion.\u003c\/p\u003e\n\u003ch2\u003eFrequently Asked Questions\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is a T75 cell culture flask used for?\u003c\/summary\u003e\n\u003cp\u003eA T75 cell culture flask is designed for the growth, maintenance, and expansion of adherent mammalian cells. With a 75 cm² growth surface, it is commonly used for routine cell culture, cell line maintenance, transfection experiments, virus production, and preparation of cells for downstream assays.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails open=\"\"\u003e\n\u003csummary\u003eWhat does TC-treated mean on a cell culture flask?\u003c\/summary\u003e\n\u003cp\u003eTC-treated (Tissue Culture Treated) refers to a surface modification process that increases the hydrophilicity of the flask surface. This treatment improves cell attachment and spreading, making TC-treated flasks ideal for adherent cell lines such as HEK293, CHO, Vero, HeLa, and fibroblast cells.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the growth area of a T75 flask?\u003c\/summary\u003e\n\u003cp\u003eA T75 flask provides a growth surface area of 75 cm² and a maximum volume of 245 mL. This size is commonly used for routine cell expansion and offers a balance between culture capacity and laboratory handling convenience.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails open=\"\"\u003e\n\u003csummary\u003eWhat is the recommended working volume for a T75 cell culture flask?\u003c\/summary\u003e\n\u003cp\u003eAlthough the maximum volume is 245 mL, most cell culture protocols recommend using approximately 15–30 mL of culture medium in a T75 flask. The optimal volume depends on the cell type and specific experimental requirements.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the difference between a vented cap and a sealed cap cell culture flask?\u003c\/summary\u003e\n\u003cp\u003eA vented cap contains a 0.2 μm filter that allows sterile gas exchange while helping prevent contamination. Vented cap flasks are typically recommended for incubator-based mammalian cell culture. Sealed cap flasks are better suited for transport, storage, or applications where gas exchange is not required.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhy is a 0.2 μm vent filter important in cell culture flasks?\u003c\/summary\u003e\n\u003cp\u003eThe 0.2 μm filter allows carbon dioxide and oxygen exchange while reducing the risk of microbial contamination. Proper gas exchange helps maintain stable culture conditions and supports healthy cell growth in CO₂ incubators.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan this T75 flask be used for HEK293, CHO, Vero, or HeLa cells?\u003c\/summary\u003e\n\u003cp\u003eYes. The TC-treated surface is suitable for a wide range of adherent mammalian cell lines, including HEK293, CHO, Vero, HeLa, MDCK, fibroblasts, stem cells, and many other attachment-dependent cell cultures.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eAre Biofargo T75 cell culture flasks sterile?\u003c\/summary\u003e\n\u003cp\u003eYes. Biofargo T75 Cell Culture Flasks are individually packaged and sterilized by irradiation. The flasks are supplied ready to use and are suitable for aseptic cell culture applications.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eAre these cell culture flasks DNase-free and RNase-free?\u003c\/summary\u003e\n\u003cp\u003eYes. Biofargo T75 flasks are manufactured under strict quality control procedures and are supplied DNase-free and RNase-free, helping protect sensitive cell culture and molecular biology workflows.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat material is used to manufacture the flask?\u003c\/summary\u003e\n\u003cp\u003eThe flask body is made from high-clarity polystyrene (PS), providing excellent visibility for microscopic observation and routine monitoring of cell morphology and confluency.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan these T75 flasks be stacked during incubation?\u003c\/summary\u003e\n\u003cp\u003eYes. The flask design allows stable stacking, helping optimize incubator space while maintaining convenient handling during routine cell culture operations.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat types of applications are T75 tissue culture flasks commonly used for?\u003c\/summary\u003e\n\u003cp\u003eT75 tissue culture flasks are widely used for adherent cell culture, cell expansion, transfection studies, recombinant protein expression, virus propagation, vaccine research, stem cell culture, toxicity testing, and general life science research.\u003c\/p\u003e\n\u003c\/details\u003e","brand":"Cellprobio","offers":[{"title":"18Bags\/Case(90Pcs\/Case)","offer_id":51539739082933,"sku":"803075","price":174.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20250908_1.jpg?v=1757474411","url":"https:\/\/biofargo.com\/products\/t75-cell-culture-flask-with-seal-cap-tc-treated-sterile","provider":"Biofargo","version":"1.0","type":"link"}