{"product_id":"superprep-ii-cell-lysis-rt-kit-for-qpcr","title":"SuperPrep II Cell Lysis \u0026 RT Kit for qPCR-Toyobo","description":"\u003cdiv style=\"font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif; color: #333; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 10px;\"\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDescription\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #f9f9f9; padding: 20px; border-radius: 4px; border: 1px solid #eee;\"\u003e\n\u003cp style=\"margin-top: 0;\"\u003eSuperPrep™ II Cell Lysis \u0026amp; RT Kit for qPCR (Code No. SCQ-401) consists of \"Lysis Reagents\" and \"RT Reagents\" for synthesis of cDNA templates for real-time PCR assays.\u003c\/p\u003e\n\u003cp\u003e\"Lysis Reagents\" prepares cell lysates containing RNAs that can be used as templates for reverse transcription. \"RT Reagents\" contains reagents for reverse transcription, optimized for efficient cDNA synthesis from crude lysates. The synthesized cDNA can be applied to real-time PCR directly. This assay system is suitable for high-throughput assays. In the previous version, it was necessary to add Stop Solution after adding Lysis Solution. In SuperPrep™ II, cell lysates with lysis solution can be used as templates for cDNA synthesis directly. Moreover, high sensitivity detection is possible from a wide variety of mammalian cells than in the previous version. With this assay system, it is possible to synthesize template cDNA for real-time PCR from cultured cells conveniently and quickly.\u003c\/p\u003e\n\u003cp\u003eSuperPrep™ II Cell Lysis Kit for qPCR (Code No. SCQ-501) is an option of \"Lysis Reagents\". The cell lysate prepared by \"Lysis Reagents\" can be applied to one-step real-time PCR.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/pic01_a295c7eb-6f7f-426c-bbef-e61fa10671d5.png?v=1775205291\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eFeatures\u003c\/h2\u003e\n\u003cul style=\"list-style-type: none; padding-left: 0;\"\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e RNA purification is not necessary.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e High-quality cDNA can be obtained from cell lysates.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e Reduction of dispersion on high-throughput assay.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e Various real-time PCR regents can be applied.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eSpecification\u003c\/h2\u003e\n\u003cdiv style=\"overflow-x: auto;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 600px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eCategory\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eItem\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; font-weight: bold; background-color: #fcfcfc;\"\u003eStorage condition\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: center;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eStore at -20ºC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eComponents\u003c\/h2\u003e\n\u003cdiv style=\"overflow-x: auto;\"\u003e\n\u003cp\u003e\u003cstrong\u003eTable 1.SuperPrep™ II Cell Lysis \u0026amp; RT Kit for qPCR (Code No.SCQ-401, SCQ-401S)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 600px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eLysis Reagents\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eSCQ-401\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eSCQ-401S (SAMPLE)\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eLysis Solution\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e6.5 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e1.3 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003egDNA Remover\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e33 µL\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e6.6 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eRNase Inhibitor\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e110 µL\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e22 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 600px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eRT Reagents\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eSCQ-401\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eSCQ-401S (SAMPLE)\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e5 × RT Master Mix\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e860 µL\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e172 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e5 × RT Master Mix no-RT Control\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e86 µL\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e17 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eNuclease-free Water\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e1.7 mL × 2\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e680 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003eTable 2.SuperPrep™ II Cell Lysis Kit for qPCR (Code No.SCQ-501)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv style=\"overflow-x: auto;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 600px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eItem\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eLysis Solution\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e6.5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003egDNA Remover\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e33 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eRNase Inhibitor\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e110 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eApplication\u003c\/h2\u003e\n\u003cp style=\"padding-left: 5px;\"\u003eDNA synthesis for real-time PCR from mammalian cultured cells(Code No. SCQ-401)\u003c\/p\u003e\n\u003cp style=\"padding-left: 5px;\"\u003eTotal RNA preparation for one-step real-time PCR from mammalian cultured cells (Code No. SCQ-501)\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eApplication Data\u003c\/h2\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 1.High-quality cDNA can be obtained from several kind of cell lysates.\u003c\/h3\u003e\n\u003cp\u003eThe optimized lysis solution efficiently inhibits RNA degradation during treatment. RNA in the lysate is stable on ice for at least 6 h. High-quality cDNA can be synthesized using highly efficient reverse transcriptase \"ReverTra Ace™\" with low contamination of genomic DNA because of preceding DNase I treatment. The reverse transcriptase is supplied as a master mix reagent containing optimally mixed primers (random and oligo dT) to achieve effective cDNA synthesis.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTable 1 Cells tested by this system\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv style=\"overflow-x: auto; margin: 20px 0;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 900px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center; width: 4.53109%;\"\u003e \u003c\/th\u003e\n\u003cth style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center; width: 11.6965%;\"\u003eCall Name\u003c\/th\u003e\n\u003cth style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center; width: 15.1739%;\"\u003e\n\u003cp\u003eAdherent\u003cbr\u003eNon-adherent\u003c\/p\u003e\n\u003c\/th\u003e\n\u003cth style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center; width: 10.6428%;\"\u003eSpecies\u003c\/th\u003e\n\u003cth style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center; width: 42.5711%;\"\u003eRemarks\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center; width: 4.53109%;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 11.6965%;\"\u003eHPA\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 15.1739%;\"\u003eAdherent\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 10.6428%;\"\u003eH.sapiens\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 42.5711%;\"\u003epreadipocytes (primary cell)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center; width: 4.53109%;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 11.6965%;\"\u003eHEK\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 15.1739%;\"\u003eAdherent\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 10.6428%;\"\u003eH.sapiens\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 42.5711%;\"\u003eepidermal keratinocytes (primary cell)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center; width: 4.53109%;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 11.6965%;\"\u003eHA\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 15.1739%;\"\u003eAdherent\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 10.6428%;\"\u003eH.sapiens\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 42.5711%;\"\u003eastrocytes (primary cell)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center; width: 4.53109%;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 11.6965%;\"\u003eHDF\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 15.1739%;\"\u003eAdherent\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 10.6428%;\"\u003eC. griseus\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 42.5711%;\"\u003edermal fibroblasts (primary cell)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center; width: 4.53109%;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 11.6965%;\"\u003eHBEpC\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 15.1739%;\"\u003eAdherent\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 10.6428%;\"\u003eH.sapiens\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 42.5711%;\"\u003ebronchial epithelial cells (primary cell)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center; width: 4.53109%;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 11.6965%;\"\u003eHUVEC\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 15.1739%;\"\u003eAdherent\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 10.6428%;\"\u003eH.sapiens\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 42.5711%;\"\u003eumbilical vein endothelial cells (primary cell)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center; width: 4.53109%;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 11.6965%;\"\u003eHPAEC\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 15.1739%;\"\u003eAdherent\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 10.6428%;\"\u003eH.sapiens\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 42.5711%;\"\u003epulmonary artery endothelial cells (primary cell)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center; width: 4.53109%;\"\u003e8\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 11.6965%;\"\u003eHC\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 15.1739%;\"\u003eAdherent\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 10.6428%;\"\u003eH.sapiens\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 42.5711%;\"\u003echondrocytes (primary cell)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center; width: 4.53109%;\"\u003e9\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 11.6965%;\"\u003eHOb\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 15.1739%;\"\u003eAdherent\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 10.6428%;\"\u003eH.sapiens\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 42.5711%;\"\u003eosteoblasts (primary cell)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center; width: 4.53109%;\"\u003e10\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 11.6965%;\"\u003eHSkMC\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 15.1739%;\"\u003eAdherent\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 10.6428%;\"\u003eH.sapiens\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 42.5711%;\"\u003eskeletal muscle cells (primary cell)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center; width: 4.53109%;\"\u003e11\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 11.6965%;\"\u003eHAOSMC\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 15.1739%;\"\u003eAdherent\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 10.6428%;\"\u003eH.sapiens\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 42.5711%;\"\u003eAortic smooth muscle cells (primary cell)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center; width: 4.53109%;\"\u003e12\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 11.6965%;\"\u003eHFDPC\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 15.1739%;\"\u003eAdherent\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 10.6428%;\"\u003eH.sapiens\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 42.5711%;\"\u003ehair follicle dermal papilla Cells (primary cell)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center; width: 4.53109%;\"\u003e13\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 11.6965%;\"\u003eHeLa S3\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 15.1739%;\"\u003eAdherent\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 10.6428%;\"\u003eH.sapiens\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 42.5711%;\"\u003ecervix carcinoma cell line\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center; width: 4.53109%;\"\u003e14\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 11.6965%;\"\u003eHepG2\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 15.1739%;\"\u003eAdherent\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 10.6428%;\"\u003eH.sapiens\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 42.5711%;\"\u003ehepatocellular carcinoma cell line\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center; width: 4.53109%;\"\u003e15\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 11.6965%;\"\u003eJurkat\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 15.1739%;\"\u003eNon-adherent\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 10.6428%;\"\u003eH.sapiens\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 42.5711%;\"\u003eT lymphocyte cell line\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center; width: 4.53109%;\"\u003e16\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 11.6965%;\"\u003eK562\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 15.1739%;\"\u003eNon-adherent\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 10.6428%;\"\u003eH.sapiens\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 42.5711%;\"\u003emyelogenous leukemia cell line\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center; width: 4.53109%;\"\u003e17\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 11.6965%;\"\u003eTHP-1\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 15.1739%;\"\u003eNon-adherent\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 10.6428%;\"\u003eH.sapiens\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 42.5711%;\"\u003eacute monocytic leukemia cell line\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center; width: 4.53109%;\"\u003e18\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 11.6965%;\"\u003eU937\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 15.1739%;\"\u003eNon-adherent\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 10.6428%;\"\u003eH.sapiens\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 42.5711%;\"\u003eleukemic monocyte lymphoma cell line\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center; width: 4.53109%;\"\u003e19\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 11.6965%;\"\u003eHMNC\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 15.1739%;\"\u003eNon-adherent\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 10.6428%;\"\u003eH.sapiens\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; width: 42.5711%;\"\u003eMononuclar cells (primary cell)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 2.Stability test of the cell lysates.\u003c\/h3\u003e\n\u003cp\u003ecDNA were synthesized from lysates that had been left on ice for 0–24 h after lysing of 4×104 HeLa and U937 cells using SuperPrep™. β-actin genes were detected using TaqMan™ real-time PCR assay with THUNDERBIRD™ Probe qPCR Mix (Code No. QPS-101). The results were compared with that from the other company’s system (Company A). The results suggest that the RNA in the cell lysates is stable for at least 2 h. Lysates from U937 cells showed higher RNase activity than the other cells and tended to deteriorate in storage over 2 h.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/pic02_329bd2db-2fed-48d2-b79f-0b3c274a0544.png?v=1775205291\"\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003eNOTE:\u003c\/strong\u003e\u003cbr\u003eRNase activity depends on the type and number of cells. The cell lysates should be placed on ice after preparation and cDNA should be synthesized immediately after preparing the lysates to minimize RNA degradation.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 3.Various real-time PCR reagents can be applied.\u003c\/h3\u003e\n\u003cp\u003eThe synthesis cDNA can be used in various real-time PCR assay (TaqMan™ probe, SYBR® Green etc.).\u003cbr\u003eIn addition, the cell lysate can be applied to one-step real-time PCR reagents.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/pic03_ac9c7b7e-0280-4a1e-b06f-632fab92a802.png?v=1775205291\"\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003eTaqMan™ Probe assay using cDNA synthesized from cell lysates prepared by SuperPrep™\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ecDNAs were synthesized using the cell lysates (8 µL) prepared from 7.5x104, 5x104, 1x104, 1x103, 1x102 and 1x10] HeLa S3 cells by SuperPrep™ in 40 µL reaction. β-actin genes were detected by various real-time PCR reagents with TaqMan™ real-time PCR assay. Successful amplifications were obtained from all reagents tested and the THUNDERBIRD™ Probe qPCR Mix tended to show a better Ct than the other tested methods.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 4.Evaluation of the assay variation\u003c\/h3\u003e\n\u003cp\u003eHeLa S3 cells were incubated with or without 100 nM phorbol 12-myristate 13-acetate (PMA) for 24 h after seeding at 2 ×104 cells\/well in a 96-well culture plate. cDNA were synthesized from the lysates prepared from the cells washed with PBS(-). IL-6, IL-1β and β-actin genes were detected by TaqMan™ real-time PCR assay with THUNDERBIRD™ Probe qPCR Mix (Code No. QPS-101). After compensation of the Cts of IL-6 and IL-1β by that of β-actin, the ΔΔCt between with or without PMA and Z’ factors* were calculated.\u003c\/p\u003e\n\u003cp\u003eZ’ factors from SuperPrep™ were superior to that from the other system (Company A).\u003c\/p\u003e\n\u003cp\u003e*The Z’ factor is a simple statistical parameter that is used to assess the quality of high-throughput screening (HTS) assays. A Z’ score of ≥0.5 is generally considered to indicate good quality Z’ can be calculated by the following formula.\u003c\/p\u003e\n\u003cp\u003eZ’= 1-3 x [Δ Ct(+) standard deviation + Δ Ct(-) standard deviation]\/| Δ Δ Ct|\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/pic04_pc.png?v=1775205291\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDocuments\u003c\/h2\u003e\n\u003cdiv style=\"border: 1px solid #eee; background-color: #f9f9f9; padding: 24px; border-radius: 8px;\"\u003e\n\u003cdiv style=\"display: flex; flex-wrap: wrap; gap: 15px;\"\u003e\n  \u003ca style=\"display: inline-block; background-color: #003366; color: #ffffff; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold;\" rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/SCQ-401_501.pdf?v=1775799967\" target=\"_blank\"\u003e Download Manual \u003c\/a\u003e \n  \n  \u003ca style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/SCQ-401_SDS.pdf?v=1775799966\" target=\"_blank\"\u003e Download SDS \u003c\/a\u003e\n\n\u003ca style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" rel=\"noopener noreferrer\" href=\"https:\/\/biofargo.com\/pages\/contact-us?srsltid=AfmBOorrYySVXlrC6mOen85FBiTKooBmPEyrEjjsqXyC0kKPRm7fDFPV\" target=\"_blank\"\u003e Request COA \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Toyobo","offers":[{"title":"100Rxn","offer_id":52417364492469,"sku":"SCQ-401","price":496.8,"currency_code":"USD","in_stock":true},{"title":"500Rxn","offer_id":52417364525237,"sku":"SCQ-401X5","price":1987.2,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/SuperPrep_II_Cell_Lysis_RT_Kit_for_qPCR-Toyobo.jpg?v=1777517312","url":"https:\/\/biofargo.com\/products\/superprep-ii-cell-lysis-rt-kit-for-qpcr","provider":"Biofargo","version":"1.0","type":"link"}