Description

The Super Plant Genomic DNA Kit adopts a highly efficient, DNA-specific adsorption spin column and a unique buffer system, which can separate and purify high-quality genomic DNA from a variety of plant tissues. The unique precipitation solution can precipitate and remove the protein, polysaccharide, phenol and other impurities in the polysaccharides & polyphenolics-rich plant samples. The extracted genomic DNA has high purity, stable and reliable quality.

Features

Simple and fast: Ultra-pure gDNA could be obtained within 1 hour.
Wide applications: Suitable for various plant tissues, especially for polysaccharides & polyphenolics-rich plants.
Non-toxic: Safe operation because no need of phenol or chloroform for extraction.
High purity and efficiency: Ultra-pure DNA can be obtained efficiently, which can be applied directly in molecular biology experiments such as chip hybridization, NGS, etc.

Applications

Suitable for a variety of regular operations, including restriction enzyme digestion, PCR, library construction, chip hybridization and Southern blot.

Components

Buffer GA: 30 ml
Buffer GHL: 20 ml
Buffer GDZ: 45 ml
Buffer PWD: 20 ml
Proteinase K: 1 ml
MagAttract Suspension GH: 0.75 ml
Buffer TB: 15 ml

Storage

This kit can be stored dry for 12 months under room temperature (15-25℃). For long-term storage, store at 2-8℃. When stored at 2-8℃, precipitation might be formed in buffer. Place the buffers at room temperature for a period of time before use, and if necessary, incubate the buffers in a 37 ℃ water bath for 10 minutes to dissolve the precipitation.

Case Study

DNA was extracted from leaves of 100 mg Malus spectabilis and peach tree with Super Plant Genomic Kit and relevant kits of supplier A and Q according to the instructions. . The experimental results show that Super Plant Genomic Kit has higher DNA extraction yield.

DNA was extracted from laves of 100 mg various plant tissues with Super Plant Genomic Kit according to the instruction. The experimental results show that Super Plant Genomic Kit can be used to extract a variety of complex plant samples,including polysaccharides & polyphenolics-rich plant samples, with high extraction yield and purity.

Super Plant Genomic DNA Kit-Tiangen