Description

Super GelBlueTM is a highly sensitive, stable, non-toxic, non-mutagenic fluorescent nucleic acid dye (working concentration). Super GelBlueTM can replace the highly toxic EtBr. It can be excited by 488 nm laser, and observed directly by blue light cutter or scanner.

Due to its unique molecular structure, Super GelBlueTM can not affect the migration of DNA bands. Even if for the large amount of DNA, it can get a good effect of strip separation.

Protocol

Pre-cast Protocol for Agarose Gels (same as EB)

Add Super GelBlueTM to molten agarose at 1× final concentration. For example, add 5 μL of 10,000× Super GelBlueTM to 50 mL agarose. Super GelBlueTM can be directly added into hot gel solution. And it also can be mixed with TAE or TBE buffer containing agarose powder in advance before heating.

Load samples and run gels according to your standard protocol.

Post-Stain Protocol

Run gel as usual according to your standard protocol.
Place the gel in a suitable container. Add a sufficient amount of 3× Super GelBlueTM solution to submerge the gel.(i.e., add 15 μL 10,000×Super GelBlueTM and 5 mL 1 M NaCl to 45 mL H2O).
Agitate the gel gently at room temperature for ~30 min. For polyacrylamide gels, typical staining time is 30 min to 1 hour with gels of higher acrylamide content requiring longer staining time.

Notes

The gel presents light orange red when we use pre-cast. After electrophoresis, we can see the uneven color of the gel, which is normal and does not affact the result.
Super GelBlueTM is more suitable for blue light than UV imaging system.
Post-staining solution can be reused at least 3 times. Store staining solution at room temperature protected from light.

Fluorescent Nucleic Acid Gel Staining Reagent-Uelandy S2019S