Description

Biofargo Sudan Black B is a fat-soluble biological stain and laboratory reagent used for staining neutral lipids and lipoproteins, blocking autofluorescence in fixed tissues, and enhancing contrast in histochemical and microscopy analyses. Ideal for research use only in histology, lipid studies, and cellular imaging applications.

Sudan black B: Sudan Black B was found to eliminate lipofuscin-like autofluorescence in mammalian neural tissue without adversely affecting other fluorescent labels. It was also found to reduce autofluroescence in archival formaldehyde-fixed paraffin-embedded myocardium tissue. Sudan Black B was used to detect native and oxidized low density lipoproteins (LDLs) after separation by capillary isotachophoresis (CITP). It has been used for staining fat in animal tissues and in bacteria (Burdon's method), as well as the lipid portion of lipoprotein in polyacrylamide gel electrophoresis. It has also been used for staining lipofuchsin.

Specifications

Item	Value
Product Name	Sudan Black B
Grade	Indicator
Chemical Formula	C₂₉H₂₄N₆
Molecular Weight	456.55
CAS No.	4197-25-5
Appearance	Very Dark Brown to Black Crystalline Powder
Identification	Conforms to Structure and Standard
Melting Point	118–126℃
Dye Content	100 ± 5%
Loss on Drying	<5.0%
Lambda Max	598 (412) nm in Ethanol
Storage	Room Temperature. Protect from Moisture

Features

High-Quality Sudan Black B – reliable fat-soluble azo dye for biological staining and histochemical applications.
Effective Lipid Staining – stains neutral triglycerides and lipoproteins in tissue sections and cellular samples.
Autofluorescence Blocking – reduces lipofuscin-like autofluorescence in fluorescent microscopy workflows.
Versatile Research Reagent – suitable for histology, microscopy, morphological studies, and diagnostic research.
Research Use Only – optimized laboratory reagent for scientific investigations, not for clinical or therapeutic use.

Applications

Biofargo Sudan Black B is widely used as a biological staining reagent and laboratory dye for:

Histological Lipid Staining – visualization of neutral fats and lipoprotein distributions in tissues.
Autofluorescence Suppression – effective blocking of background fluorescence in formalin-fixed samples.
Microscopy & Imaging Studies – used in light microscopy and fluorescence imaging workflows.
Cellular & Tissue Morphology Research – aids in differentiating lipid-rich structures during sample analysis.
Biochemical and Diagnostic Research – research use only dye for histology, pathology research, and lipid analysis.

Documents

INFO

COA

SDS

Disclaimer: For laboratory research use only.

Sudan black B Indicator Grade