{"product_id":"stubby-udi-primer-kit-for-illumina-pe-adapter-plus-set4-289-384-12330es","title":"Stubby UDI Primer Kit for Illumina, PE adapter plus, Set4 ( 289-384)-12330ES","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eStubby UDI Primer Kit for Illumina is a dedicated linker kit for library construction on the Illumina high-throughput sequencing platform, including PE Adapter and UDI Primer used in next-generation sequencing library construction. The UDI Primer is a master mix of i5 and i7 Primer used with the DNA Library Prep Kit for Illumina, and 12327-12330 are available in plate packs with 96 indexes each, allowing the construction of up to 384 double-ended unique Index-labeled libraries. All reagents provided in the kit undergo rigorous quality control and functional verification to maximize library construction stability and repeatability.\u003c\/p\u003e\n\u003ch2\u003eComponents\u003cbr\u003e\n\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eNo.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eName\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003e96 × 1 T\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003e96 × 2 T\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003e96 × 4 T\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" style=\"text-align: center;\"\u003e12327\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003ePE Adapter\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e336 μL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e672 μL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2 × 672 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eUDI Primer 001–096\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e5 μL each\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e10 μL each\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e20 μL each\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" style=\"text-align: center;\"\u003e12328\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003ePE Adapter\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e336 μL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e672 μL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2 × 672 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eUDI Primer 097–192\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e5 μL each\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e10 μL each\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e20 μL each\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" style=\"text-align: center;\"\u003e12329\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003ePE Adapter\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e336 μL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e672 μL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2 × 672 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eUDI Primer 193–288\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e5 μL each\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e10 μL each\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e20 μL each\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" style=\"text-align: center;\"\u003e12330\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003ePE Adapter\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e336 μL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e672 μL\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2 × 672 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eUDI Primer 289–384\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e5 μL each\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e10 μL each\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e20 μL each\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eShipping and Storage\u003c\/h2\u003e\n\u003cp\u003eAll the components are shipped with dry ice and can be stored at -15℃~ -25℃ for 2 years.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eThe concentration of PE Adapter in this kit is 15 μM, and the amount of linker used for individual library construction is adjusted according to the kit used.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe PE Adapter provided with this kit is a general-purpose short adapter that requires PCR amplification to obtain a complete library.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eUDI Primer provides Index sequence tags at a concentration of 12.5 μM for sample differentiation during high-throughput sequencing.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDo not heat the adaptors, let it dissolve slowly at room temperature, and the laboratory temperature is best set to 20-25 °C. It is recommended to store it in aliquots avoiding repeated freeze-thaw and store it at 4°C for a short time.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe DNA library structure using the Hieff NGS® Stubby UDI Primer Kit for Illumina® kit is as follows:\u003cbr\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/7cee-168483330621879434_480x480_8f7b67ec-a9b0-4590-be9e-e47b0b30f29e.jpg?v=1776396777\"\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFor your safety and health, please wear a lab coat and wear disposable gloves.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThis product is for scientific research purposes only!\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSequence information\u003c\/h2\u003e\n\u003cp\u003e PE Adapter for Illumina：\u003c\/p\u003e\n\u003cp\u003e5´-\/5Phos\/GATCGGAAGAGCACACGTCTGAACTCCAGT*C-3´\u003c\/p\u003e\n\u003cp\u003e5´-ACACTCTTTCCCTACACGACGCTCTTCCGATC*T-3´\u003c\/p\u003e\n\u003cp\u003ei5 Index Primer for Illumina：\u003c\/p\u003e\n\u003cp\u003e5´-AATGATACGGCGACCACCGAGATCTACAC[i5 Index]ACACTCTTTCCCTACACGACGCTCTTCCGATCT-3´\u003c\/p\u003e\n\u003cp\u003ei7 Index Primer for Illumina：\u003c\/p\u003e\n\u003cp\u003e5´-CAAGCAGAAGACGGCATACGAGAT[i7 Index]GTGACTGGAGTTCAGACGTGTGCTCTTCCGATC-3´\u003c\/p\u003e\n\u003cp\u003e[i5 Index] 8 bp i5 sequence，[i7 Index]8 bp i7 sequence\u003c\/p\u003e\n\u003cp\u003eThe corresponding locations of each primer in 96-well plate are shown:‘’\u003cbr\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/319c-168483329085540421_480x480_263bd221-e71e-4bd5-af18-48ba2ab40ac3.jpg?v=1776396796\"\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/12330-Manual-Ver.EN20260327.pdf?v=1776403250\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Yeasenbio","offers":[{"title":"96T","offer_id":52439374594229,"sku":"12330ES01","price":423.0,"currency_code":"USD","in_stock":true},{"title":"192T","offer_id":52439374626997,"sku":"12330ES02","price":841.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Univ_8baa1596-48a0-4ecb-ae42-4ae9d0769e29_1.jpg?v=1776403118","url":"https:\/\/biofargo.com\/products\/stubby-udi-primer-kit-for-illumina-pe-adapter-plus-set4-289-384-12330es","provider":"Biofargo","version":"1.0","type":"link"}