{"product_id":"stripping-protocol-stripsure™-western-blot-stripping-buffer","title":"Stripping Protocol (StripSure™) - Western Blot Stripping Buffer","description":"\u003ch1 dir=\"ltr\"\u003e\n\u003cspan\u003eStripSure™ Western Blot Stripping Buffer\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h1\u003e\n\u003cp dir=\"ltr\"\u003e\u003cstrong\u003eStripSure™ Western Blot Stripping Buffer — Ready-to-Use, 20-Minute Protocol\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eStripSure™ is a ready-to-use stripping buffer formulated to efficiently remove primary and secondary antibodies from Western blot membranes while fully preserving membrane-bound target proteins for reliable re-probing. It's compatible with PVDF, nitrocellulose, and nylon membranes, and delivers complete antibody removal in a single 20-minute incubation at room temperature with only gentle agitation required — no heat, no harsh chemicals, no lengthy prep.\u003c\/span\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003ePerformance testing confirms that StripSure™ eliminates detectable signal from previously probed antibodies (validated against anti-ATG7) while leaving target proteins fully intact on the membrane. Re-probing after stripping produced strong, specific signals with both a non-phospho antibody (β-actin) and a phospho-specific antibody (phospho-STAT3, Tyr705) — demonstrating that StripSure™ is gentle enough for even sensitive phospho-specific detection workflows.\u003c\/span\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eBecause StripSure™ ships ready-to-use, there's no dilution or multi-reagent mixing involved. Simply immerse the membrane, incubate, rinse, and re-probe. This makes it a reliable choice for labs that need to reprobe the same membrane for multiple targets without sacrificing signal quality or membrane integrity.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eKey Features \u0026amp; Benefits\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli dir=\"ltr\"\u003e\n\u003cp dir=\"ltr\" role=\"presentation\"\u003e\u003cspan\u003eEfficient, complete removal of both primary and secondary antibodies\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli dir=\"ltr\"\u003e\n\u003cp dir=\"ltr\" role=\"presentation\"\u003e\u003cspan\u003eCompatible with phospho-specific antibody re-probing\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli dir=\"ltr\"\u003e\n\u003cp dir=\"ltr\" role=\"presentation\"\u003e\u003cspan\u003ePreserves membrane-bound target proteins across multiple stripping cycles\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli dir=\"ltr\"\u003e\n\u003cp dir=\"ltr\" role=\"presentation\"\u003e\u003cspan\u003eEnables multiple rounds of re-probing from a single membrane\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli dir=\"ltr\"\u003e\n\u003cp dir=\"ltr\" role=\"presentation\"\u003e\u003cspan\u003eReady-to-use liquid formulation — do not dilute\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli dir=\"ltr\"\u003e\n\u003cp dir=\"ltr\" role=\"presentation\"\u003e\u003cspan\u003eFast 20-minute protocol at room temperature (20–25°C)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli dir=\"ltr\"\u003e\n\u003cp dir=\"ltr\" role=\"presentation\"\u003e\u003cspan\u003eCompatible with PVDF, nitrocellulose, and nylon membranes\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli dir=\"ltr\"\u003e\n\u003cp dir=\"ltr\" role=\"presentation\"\u003e\u003cspan\u003eIdeal for chemiluminescent and fluorescent detection systems\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eHow It Works\u003c\/span\u003e\u003c\/h2\u003e\n\u003col\u003e\n\u003cli dir=\"ltr\"\u003e\n\u003cp dir=\"ltr\" role=\"presentation\"\u003e\u003cspan\u003eInitial wash\u003c\/span\u003e\u003cspan\u003e — Rinse the membrane once with deionized water to remove residual detection reagents.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli dir=\"ltr\"\u003e\n\u003cp dir=\"ltr\" role=\"presentation\"\u003e\u003cspan\u003eStripping\u003c\/span\u003e\u003cspan\u003e — Gently mix StripSure™ until homogeneous (do not dilute) and completely immerse the membrane, ensuring it's fully covered.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli dir=\"ltr\"\u003e\n\u003cp dir=\"ltr\" role=\"presentation\"\u003e\u003cspan\u003eIncubation\u003c\/span\u003e\u003cspan\u003e — Incubate for 20 minutes at room temperature (20–25°C) with gentle agitation.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli dir=\"ltr\"\u003e\n\u003cp dir=\"ltr\" role=\"presentation\"\u003e\u003cspan\u003eRinsing\u003c\/span\u003e\u003cspan\u003e — Remove the stripping buffer and rinse 3 times with deionized water, or 3 times with TBST (recommended for sensitive applications).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli dir=\"ltr\"\u003e\n\u003cp dir=\"ltr\" role=\"presentation\"\u003e\u003cspan\u003eRe-equilibration\u003c\/span\u003e\u003cspan\u003e — Equilibrate the membrane in TBST for 5 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli dir=\"ltr\"\u003e\n\u003cp dir=\"ltr\" role=\"presentation\"\u003e\u003cspan\u003eRe-probing\u003c\/span\u003e\u003cspan\u003e — Proceed with your standard Western blot workflow: primary antibody incubation, wash, secondary antibody incubation, wash, and detection.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003cb\u003e\u003cbr\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eImportant:\u003c\/span\u003e\u003cspan\u003e Ensure the membrane is completely submerged in StripSure™ throughout the stripping step for consistent, even results.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eSpecifications\u003c\/span\u003e\u003c\/h2\u003e\n\u003cdiv dir=\"ltr\" align=\"left\"\u003e\n\u003ctable style=\"width: 99.9973%; height: 628.33px;\"\u003e\n\u003ccolgroup\u003e \u003ccol style=\"width: 33.0465%;\"\u003e \u003ccol style=\"width: 65.7487%;\"\u003e \u003c\/colgroup\u003e\n\u003cthead\u003e\n\u003ctr style=\"height: 35.5957px;\"\u003e\n\u003cth scope=\"col\" style=\"height: 35.5957px;\"\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eAttribute\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/th\u003e\n\u003cth scope=\"col\" style=\"height: 35.5957px;\"\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eDetail\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 35.5957px;\"\u003e\n\u003ctd style=\"height: 35.5957px;\"\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eProduct Name\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 35.5957px;\"\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eStripSure™ Western Blot Stripping Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5957px;\"\u003e\n\u003ctd style=\"height: 35.5957px;\"\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eBrand\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 35.5957px;\"\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eBioFargo Inc.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5957px;\"\u003e\n\u003ctd style=\"height: 35.5957px;\"\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eFormat\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 35.5957px;\"\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eReady-to-use liquid (do not dilute)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5957px;\"\u003e\n\u003ctd style=\"height: 35.5957px;\"\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eCompatible Membranes\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 35.5957px;\"\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003ePVDF, nitrocellulose, nylon\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 55.1953px;\"\u003e\n\u003ctd style=\"height: 55.1953px;\"\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eRecommended Detection Systems\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 55.1953px;\"\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eChemiluminescent and fluorescent imaging\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5957px;\"\u003e\n\u003ctd style=\"height: 35.5957px;\"\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eIncubation Time\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 35.5957px;\"\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003e20 minutes\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5957px;\"\u003e\n\u003ctd style=\"height: 35.5957px;\"\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eIncubation Temperature\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 35.5957px;\"\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eRoom temperature (20–25°C)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5957px;\"\u003e\n\u003ctd style=\"height: 35.5957px;\"\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eAgitation\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 35.5957px;\"\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eGentle\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 55.1953px;\"\u003e\n\u003ctd style=\"height: 55.1953px;\"\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eAntibody Compatibility\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 55.1953px;\"\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003ePrimary and secondary antibodies, including phospho-specific antibodies\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 55.1953px;\"\u003e\n\u003ctd style=\"height: 55.1953px;\"\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eRinse Options\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 55.1953px;\"\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eDeionized water or TBST (TBST recommended for sensitive applications)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5957px;\"\u003e\n\u003ctd style=\"height: 35.5957px;\"\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eSize \/ Volume\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 35.5957px;\"\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003e500mL Bottle\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5957px;\"\u003e\n\u003ctd style=\"height: 35.5957px;\"\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eStorage Conditions\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 35.5957px;\"\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eRoom Temperature\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5957px;\"\u003e\n\u003ctd style=\"height: 35.5957px;\"\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eShelf Life\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 35.5957px;\"\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003e1 Year\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Screenshot_2026-08-17_at_11.50.05_AM.png?v=1786985452\" alt=\"\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Screenshot_2026-08-17_at_11.50.16_AM.png?v=1786985452\" alt=\"\"\u003e\u003cimg\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/StripSure_-_BioFargo_Inc.-072126.docx?v=1786985291\"\u003e\u003cstrong\u003eDownload Product User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Biofargo","offers":[{"title":"Default Title","offer_id":52979990364341,"sku":"BF-SS-500","price":120.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20260817-114118.jpg?v=1786984894","url":"https:\/\/biofargo.com\/products\/stripping-protocol-stripsure%e2%84%a2-western-blot-stripping-buffer","provider":"Biofargo","version":"1.0","type":"link"}