{"product_id":"ssdna-assay-kit-for-single-stranded-dna-quantification","title":"Biofargo ssDNA Assay Kit-V2","description":"\u003cstyle\u003e\n  .bf-rich .josh-faq {\n    background: #fff;\n    color: #000;\n    font-family: \"IBM Plex Sans\", sans-serif;\n  }\n\n  .bf-rich .josh-faq__inner { padding: 24px 0 40px; max-width: 100%; box-sizing: border-box; }\n  .bf-rich .josh-faq__item { border-bottom: 1px solid #e5e7eb; padding: 0; }\n  .bf-rich .josh-faq__item:first-child { border-top: 1px solid #e5e7eb; }\n\n  .bf-rich .josh-faq__question {\n    width: 100%;\n    margin: 0;\n    padding: 20px 0;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    color: #505052;\n    background: none;\n    border: 0;\n    font: 700 16px\/1.4 \"IBM Plex Sans\", sans-serif;\n    text-align: left;\n    cursor: pointer;\n    list-style: none;\n  }\n\n  .bf-rich .josh-faq__question::-webkit-details-marker { display: none; }\n  .bf-rich .josh-faq__question:hover { color: #505052; }\n\n  .bf-rich .josh-faq__icon {\n    position: relative;\n    width: 20px;\n    height: 20px;\n    flex: 0 0 20px;\n    transition: transform .25s ease;\n  }\n\n  .bf-rich .josh-faq__icon::before,\n  .bf-rich .josh-faq__icon::after {\n    position: absolute;\n    content: \"\";\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity .25s ease, transform .25s ease;\n  }\n\n  .bf-rich .josh-faq__icon::before {\n    top: 50%;\n    left: 0;\n    width: 100%;\n    height: 2px;\n    transform: translateY(-50%);\n  }\n\n  .bf-rich .josh-faq__icon::after {\n    top: 0;\n    left: 50%;\n    width: 2px;\n    height: 100%;\n    transform: translateX(-50%);\n  }\n\n  .bf-rich .josh-faq__item[open] .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .bf-rich .josh-faq__answer {\n    max-width: 860px;\n    padding: 0 0 20px;\n    color: #505052;\n    font: 400 16px\/1.6 \"IBM Plex Sans\", sans-serif;\n  }\n\n  .bf-rich .josh-faq__answer p { margin: 0 0 15px; color: #505052; font: inherit; }\n  .bf-rich .josh-faq__answer p:last-child { margin-bottom: 0; }\n  .bf-rich .josh-faq__answer ul { margin: 8px 0 12px; padding-left: 22px; }\n  .bf-rich .josh-faq__answer li { margin-bottom: 6px; font: inherit; list-style-type: disc; }\n\n  @media (max-width: 767px) {\n    .bf-rich .josh-faq__inner { padding: 24px 0 40px; }\n    .bf-rich .josh-faq__question { padding: 16px 0; font-size: 15px; }\n    .bf-rich .josh-faq__answer { font-size: 15px; }\n  }\n\u003c\/style\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eThe Biofargo ssDNA Assay Kit-V2 provides fluorescence-based quantification of single-stranded DNA (ssDNA) for molecular biology research. The reagent also responds to dsDNA and RNA; purify ssDNA before quantification when these are present.\u003c\/p\u003e\n\u003cp\u003eThe total target amount is 1–200 ng per assay. Use 1–20 µL of sample in a 200 µL final reaction and incubate for 2 minutes at 20–25 °C, protected from light. Prepare fresh working solution by mixing 1 volume of fluorescent reagent with 199 volumes of the supplied buffer (1:200 final dilution).\u003c\/p\u003e\n\u003cp\u003eAvailable formats: BF-T1008-V2-S (100 assays) and BF-T1008-V2 (1,000 assays). For research use only. Not for diagnostic or therapeutic procedures. This assay does not independently distinguish ssDNA from contaminating dsDNA or RNA.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 90%;\"\u003e\n  \u003ctbody\u003e\n    \u003ctr\u003e\n      \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eParameter\u003c\/strong\u003e\u003c\/td\u003e\n      \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eSpecification\u003c\/strong\u003e\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd style=\"text-align: center;\"\u003eBiofargo catalog numbers\u003c\/td\u003e\n      \u003ctd style=\"text-align: center;\"\u003eBF-T1008-V2-S \/ BF-T1008-V2\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd style=\"text-align: center;\"\u003eTarget\u003c\/td\u003e\n      \u003ctd style=\"text-align: center;\"\u003esingle-stranded DNA (ssDNA)\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd style=\"text-align: center;\"\u003eTotal target amount per assay\u003c\/td\u003e\n      \u003ctd style=\"text-align: center;\"\u003e1–200 ng\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd style=\"text-align: center;\"\u003eInitial sample concentration\u003c\/td\u003e\n      \u003ctd style=\"text-align: center;\"\u003e0.05–200 ng\/µL (50 pg\/µL at the lower end), depending on sample input\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd style=\"text-align: center;\"\u003eSample input\u003c\/td\u003e\n      \u003ctd style=\"text-align: center;\"\u003e1–20 µL\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd style=\"text-align: center;\"\u003eFinal assay volume\u003c\/td\u003e\n      \u003ctd style=\"text-align: center;\"\u003e200 µL per tube or well\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd style=\"text-align: center;\"\u003eWorking-solution preparation\u003c\/td\u003e\n      \u003ctd style=\"text-align: center;\"\u003ePrepare fresh working solution by mixing 1 volume of fluorescent reagent with 199 volumes of the supplied buffer (1:200 final dilution).\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd style=\"text-align: center;\"\u003eIncubation\u003c\/td\u003e\n      \u003ctd style=\"text-align: center;\"\u003e2 minutes at 20–25 °C, protected from light\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd style=\"text-align: center;\"\u003eSignal stability\u003c\/td\u003e\n      \u003ctd style=\"text-align: center;\"\u003eUp to 3 hours, protected from light\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd style=\"text-align: center;\"\u003eInstrument formats\u003c\/td\u003e\n      \u003ctd style=\"text-align: center;\"\u003eBiofargo X1 and X3; Qubit fluorometers with the matching assay; fluorescence microplate readers\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd style=\"text-align: center;\"\u003ePlate-reader settings\u003c\/td\u003e\n      \u003ctd style=\"text-align: center;\"\u003e485 nm excitation \/ 530 nm emission\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd style=\"text-align: center;\"\u003eAvailable formats\u003c\/td\u003e\n      \u003ctd style=\"text-align: center;\"\u003eBF-T1008-V2-S: 100 assays; BF-T1008-V2: 1,000 assays\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd style=\"text-align: center;\"\u003eIntended use\u003c\/td\u003e\n      \u003ctd style=\"text-align: center;\"\u003eResearch use only\u003c\/td\u003e\n    \u003c\/tr\u003e\n  \u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\u003cp\u003eConcentrated fluorescent reagent and assay buffer support freshly prepared working solution.\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp\u003eQuantifies single-stranded DNA (ssDNA) at 1–200 ng per assay.\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp\u003eThe reagent also responds to dsDNA and RNA; purify ssDNA before quantification when these are present.\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp\u003eUses 1–20 µL of sample in a 200 µL reaction.\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp\u003eTwo-minute incubation at room temperature.\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp\u003eSupports tube-based and fluorescence microplate-reader workflows.\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n    \u003cp\u003e\u003cstrong\u003eOligonucleotide and ssDNA research:\u003c\/strong\u003e Quantify purified oligonucleotides or long ssDNA. Sequence, length and contamination can affect the signal.\u003c\/p\u003e\n  \u003c\/li\u003e\n  \u003cli\u003e\n    \u003cp\u003e\u003cstrong\u003eNGS sample preparation:\u003c\/strong\u003e Measure target nucleic acid concentration for input normalization; this concentration assay does not measure fragment size, integrity or amplifiable library concentration.\u003c\/p\u003e\n  \u003c\/li\u003e\n  \u003cli\u003e\n    \u003cp\u003e\u003cstrong\u003eFluorescence microplates:\u003c\/strong\u003e Use a black 96-well plate and a multi-point standard curve at 485 nm excitation \/ 530 nm emission.\u003c\/p\u003e\n  \u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 90%;\"\u003e\n  \u003ctbody\u003e\n    \u003ctr\u003e\n      \u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent\u003c\/td\u003e\n      \u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDesignation\u003c\/td\u003e\n      \u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBF-T1008-V2-S (100 assays)\u003c\/td\u003e\n      \u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBF-T1008-V2 (1,000 assays)\u003c\/td\u003e\n      \u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFunction\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003essDNA Buffer QB-V2\u003c\/td\u003e\n      \u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eQB-V2\u003c\/td\u003e\n      \u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e20 mL\u003c\/td\u003e\n      \u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e200 mL\u003c\/td\u003e\n      \u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eWorking-solution buffer\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003essDNA Standard QS0\u003c\/td\u003e\n      \u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eQS0\u003c\/td\u003e\n      \u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e100 µL\u003c\/td\u003e\n      \u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\n      \u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlank standard; 0 ng\/µL\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003essDNA Standard QS1\u003c\/td\u003e\n      \u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eQS1\u003c\/td\u003e\n      \u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e100 µL\u003c\/td\u003e\n      \u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\n      \u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eHigh standard; 20 ng\/µL\u003c\/td\u003e\n    \u003c\/tr\u003e\n    \u003ctr\u003e\n      \u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003essDNA Fluorescent Reagent QF\u003c\/td\u003e\n      \u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eQF\u003c\/td\u003e\n      \u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e100 µL\u003c\/td\u003e\n      \u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\n      \u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eConcentrated detection reagent\u003c\/td\u003e\n    \u003c\/tr\u003e\n  \u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\u003cp\u003eShip on ice.\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp\u003eStore all components at 2–8 °C, protected from light.\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eFrequently Asked Questions (FAQs)\u003c\/h2\u003e\n\u003cdiv class=\"josh-faq\"\u003e\n  \u003csection class=\"josh-faq__inner\"\u003e\n    \u003cdiv class=\"josh-faq__list\"\u003e\n      \u003cdetails class=\"josh-faq__item\"\u003e\n        \u003csummary class=\"josh-faq__question\"\u003e\n          What is the quantification range?\n          \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\n        \u003c\/summary\u003e\n        \u003cdiv class=\"josh-faq__answer\"\u003e\n          \u003cp\u003eThe target mass range is 1–200 ng in the assay. The stated initial concentration range is 0.05–200 ng\/µL (50 pg\/µL at the lower end), depending on sample input. Initial sample concentration is measured before dilution; target mass equals initial concentration multiplied by the sample volume. These are different quantities.\u003c\/p\u003e\n        \u003c\/div\u003e\n      \u003c\/details\u003e\n      \u003cdetails class=\"josh-faq__item\"\u003e\n        \u003csummary class=\"josh-faq__question\"\u003e\n          Can dsDNA or RNA affect ssDNA results?\n          \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\n        \u003c\/summary\u003e\n        \u003cdiv class=\"josh-faq__answer\"\u003e\n          \u003cp\u003eYes. The ssDNA reagent also produces fluorescence with dsDNA and RNA. Remove these contaminants before interpreting a result as ssDNA concentration.\u003c\/p\u003e\n        \u003c\/div\u003e\n      \u003c\/details\u003e\n      \u003cdetails class=\"josh-faq__item\"\u003e\n        \u003csummary class=\"josh-faq__question\"\u003e\n          How do I prepare the working solution?\n          \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\n        \u003c\/summary\u003e\n        \u003cdiv class=\"josh-faq__answer\"\u003e\n          \u003cp\u003ePrepare fresh working solution by mixing 1 volume of fluorescent reagent with 199 volumes of the supplied buffer (1:200 final dilution).\u003c\/p\u003e\n        \u003c\/div\u003e\n      \u003c\/details\u003e\n      \u003cdetails class=\"josh-faq__item\"\u003e\n        \u003csummary class=\"josh-faq__question\"\u003e\n          Can I use this kit with Qubit fluorometers?\n          \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\n        \u003c\/summary\u003e\n        \u003cdiv class=\"josh-faq__answer\"\u003e\n          \u003cp\u003eThe supplier protocol supports use with the matching Qubit assay program. Use this kit's own standards, the specified sample volume and the correct consumables for the Qubit model. Compatibility does not imply Thermo Fisher endorsement or identical performance. For the 1X assay on older Qubit models, verify that the appropriate assay program is installed.\u003c\/p\u003e\n        \u003c\/div\u003e\n      \u003c\/details\u003e\n      \u003cdetails class=\"josh-faq__item\"\u003e\n        \u003csummary class=\"josh-faq__question\"\u003e\n          Which Biofargo fluorometers support this assay?\n          \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\n        \u003c\/summary\u003e\n        \u003cdiv class=\"josh-faq__answer\"\u003e\n          \u003cp\u003eX1 and X3 list the corresponding dsDNA or ssDNA program. The eight-position X3 uses different tubes from the single-sample X1.\u003c\/p\u003e\n        \u003c\/div\u003e\n      \u003c\/details\u003e\n      \u003cdetails class=\"josh-faq__item\"\u003e\n        \u003csummary class=\"josh-faq__question\"\u003e\n          How much sample is required?\n          \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\n        \u003c\/summary\u003e\n        \u003cdiv class=\"josh-faq__answer\"\u003e\n          \u003cp\u003eAdd 1–20 µL of sample and adjust the working solution to a final volume of 200 µL. Choose the input volume so the target mass lies within the assay range. Dilute samples that exceed the upper limit.\u003c\/p\u003e\n        \u003c\/div\u003e\n      \u003c\/details\u003e\n      \u003cdetails class=\"josh-faq__item\"\u003e\n        \u003csummary class=\"josh-faq__question\"\u003e\n          Which assay tubes should I use?\n          \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\n        \u003c\/summary\u003e\n        \u003cdiv class=\"josh-faq__answer\"\u003e\n          \u003cp\u003eUse clear, thin-wall 0.5 mL assay tubes for Biofargo X1 and Qubit 2\/3\/4. Use the instrument-specified clear 0.2 mL tubes or eight-tube strips for Biofargo X3; Qubit Flex requires its specified 0.2 mL assay tube strips. Keep the total assay volume at 200 µL.\u003c\/p\u003e\n        \u003c\/div\u003e\n      \u003c\/details\u003e\n      \u003cdetails class=\"josh-faq__item\"\u003e\n        \u003csummary class=\"josh-faq__question\"\u003e\n          How long does the assay take?\n          \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\n        \u003c\/summary\u003e\n        \u003cdiv class=\"josh-faq__answer\"\u003e\n          \u003cp\u003eIncubate for 2 minutes at 20–25 °C, protected from light, after mixing. This is the incubation time, not the total preparation and measurement time. The signal remains stable for up to 3 hours when protected from light.\u003c\/p\u003e\n        \u003c\/div\u003e\n      \u003c\/details\u003e\n      \u003cdetails class=\"josh-faq__item\"\u003e\n        \u003csummary class=\"josh-faq__question\"\u003e\n          Do I need new standards?\n          \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\n        \u003c\/summary\u003e\n        \u003cdiv class=\"josh-faq__answer\"\u003e\n          \u003cp\u003eUse the two standards supplied with this kit and the matching assay program. A new calibration for each run is recommended. Do not reuse a calibration generated with a different kit. For a plate reader, prepare a dilution series and fit a standard curve.\u003c\/p\u003e\n        \u003c\/div\u003e\n      \u003c\/details\u003e\n      \u003cdetails class=\"josh-faq__item\"\u003e\n        \u003csummary class=\"josh-faq__question\"\u003e\n          Does the reaction count equal the number of unknown samples?\n          \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\n        \u003c\/summary\u003e\n        \u003cdiv class=\"josh-faq__answer\"\u003e\n          \u003cp\u003eThe listed count is the nominal assay format. Standards, controls, repeat measurements and handling losses reduce the number of unknown samples that can be measured.\u003c\/p\u003e\n        \u003c\/div\u003e\n      \u003c\/details\u003e\n      \u003cdetails class=\"josh-faq__item\"\u003e\n        \u003csummary class=\"josh-faq__question\"\u003e\n          How should I store the kit?\n          \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\n        \u003c\/summary\u003e\n        \u003cdiv class=\"josh-faq__answer\"\u003e\n          \u003cp\u003eShip on ice. Store all components at 2–8 °C, protected from light.\u003c\/p\u003e\n        \u003c\/div\u003e\n      \u003c\/details\u003e\n      \u003cdetails class=\"josh-faq__item\"\u003e\n        \u003csummary class=\"josh-faq__question\"\u003e\n          Is this product intended for diagnostic use?\n          \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\n        \u003c\/summary\u003e\n        \u003cdiv class=\"josh-faq__answer\"\u003e\n          \u003cp\u003eNo. For research use only. Not for diagnostic or therapeutic procedures.\u003c\/p\u003e\n        \u003c\/div\u003e\n      \u003c\/details\u003e\n    \u003c\/div\u003e\n  \u003c\/section\u003e\n\u003c\/div\u003e\n\u003ch2\u003eCompatible Instruments\u003c\/h2\u003e\n\u003cp\u003eThis kit is run on the Biofargo fluorometers below.\u003c\/p\u003e\n\u003cul\u003e\n  \u003cli\u003e\u003cp\u003e\u003ca href=\"\/products\/fluorometer-x1-dna-rna-protein-quantification\"\u003e\u003cstrong\u003eBiofargo Fluorometer X1\u003c\/strong\u003e\u003c\/a\u003e — Single sample position. Select the matching assay program on the instrument.\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp\u003e\u003ca href=\"\/products\/fluorometer-x3-for-dna-rna-protein-quantification\"\u003e\u003cstrong\u003eBiofargo Fluorometer X3\u003c\/strong\u003e\u003c\/a\u003e — Eight sample positions. Select the matching assay program on the instrument.\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca target=\"_blank\" rel=\"noopener\" type=\"application\/pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/BF-T1008-V2_Product_Guide.pdf?v=1791219229\"\u003e\u003cstrong\u003eBiofargo BF-T1008-V2 Product Guide\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cscript type=\"application\/ld+json\"\u003e{\"@context\": \"https:\/\/schema.org\", \"@type\": \"FAQPage\", \"mainEntity\": [{\"@type\": \"Question\", \"name\": \"What is the quantification range?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"The target mass range is 1–200 ng in the assay. The stated initial concentration range is 0.05–200 ng\/µL (50 pg\/µL at the lower end), depending on sample input. Initial sample concentration is measured before dilution; target mass equals initial concentration multiplied by the sample volume. These are different quantities.\"}}, {\"@type\": \"Question\", \"name\": \"Can dsDNA or RNA affect ssDNA results?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Yes. The ssDNA reagent also produces fluorescence with dsDNA and RNA. Remove these contaminants before interpreting a result as ssDNA concentration.\"}}, {\"@type\": \"Question\", \"name\": \"How do I prepare the working solution?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Prepare fresh working solution by mixing 1 volume of fluorescent reagent with 199 volumes of the supplied buffer (1:200 final dilution).\"}}, {\"@type\": \"Question\", \"name\": \"Can I use this kit with Qubit fluorometers?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"The supplier protocol supports use with the matching Qubit assay program. Use this kit's own standards, the specified sample volume and the correct consumables for the Qubit model. Compatibility does not imply Thermo Fisher endorsement or identical performance. For the 1X assay on older Qubit models, verify that the appropriate assay program is installed.\"}}, {\"@type\": \"Question\", \"name\": \"Which Biofargo fluorometers support this assay?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"X1 and X3 list the corresponding dsDNA or ssDNA program. The eight-position X3 uses different tubes from the single-sample X1.\"}}, {\"@type\": \"Question\", \"name\": \"How much sample is required?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Add 1–20 µL of sample and adjust the working solution to a final volume of 200 µL. Choose the input volume so the target mass lies within the assay range. Dilute samples that exceed the upper limit.\"}}, {\"@type\": \"Question\", \"name\": \"Which assay tubes should I use?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Use clear, thin-wall 0.5 mL assay tubes for Biofargo X1 and Qubit 2\/3\/4. Use the instrument-specified clear 0.2 mL tubes or eight-tube strips for Biofargo X3; Qubit Flex requires its specified 0.2 mL assay tube strips. Keep the total assay volume at 200 µL.\"}}, {\"@type\": \"Question\", \"name\": \"How long does the assay take?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Incubate for 2 minutes at 20–25 °C, protected from light, after mixing. This is the incubation time, not the total preparation and measurement time. The signal remains stable for up to 3 hours when protected from light.\"}}, {\"@type\": \"Question\", \"name\": \"Do I need new standards?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Use the two standards supplied with this kit and the matching assay program. A new calibration for each run is recommended. Do not reuse a calibration generated with a different kit. For a plate reader, prepare a dilution series and fit a standard curve.\"}}, {\"@type\": \"Question\", \"name\": \"Does the reaction count equal the number of unknown samples?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"The listed count is the nominal assay format. Standards, controls, repeat measurements and handling losses reduce the number of unknown samples that can be measured.\"}}, {\"@type\": \"Question\", \"name\": \"How should I store the kit?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Ship on ice. Store all components at 2–8 °C, protected from light.\"}}, {\"@type\": \"Question\", \"name\": \"Is this product intended for diagnostic use?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"No. For research use only. Not for diagnostic or therapeutic procedures.\"}}]}\u003c\/script\u003e","brand":"Biofargo","offers":[{"title":"100 Assays","offer_id":53193987227829,"sku":"BF-T1008-V2-S","price":108.0,"currency_code":"USD","in_stock":true},{"title":"1000 Assays","offer_id":53193987260597,"sku":"BF-T1008-V2","price":643.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/ssdna-assay-kit-unbranded-1500.jpg?v=1789629446","url":"https:\/\/biofargo.com\/products\/ssdna-assay-kit-for-single-stranded-dna-quantification","provider":"Biofargo","version":"1.0","type":"link"}