{"product_id":"ssdna-assay-kit-for-single-stranded-dna-quantification-starlighter","title":"ssDNA Assay Kit for Single-Stranded DNA Quantification——StarLighter","description":"\u003csection\u003e\n  \u003ch2\u003eDescription\u003c\/h2\u003e\n\n  \u003cp\u003eLaboratories quantifying oligonucleotides or long single-stranded DNA may require greater sensitivity and accuracy than traditional UV A260 measurements provide. The StarLighter ssDNA Assay Kit offers an easy fluorescence-based solution for single-stranded DNA quantification across a total assay range of 1–200 ng.\u003c\/p\u003e\n\n  \u003cp\u003eDepending on the sample volume, this ssDNA quantification kit accurately detects initial ssDNA concentrations from 50 pg\/µL to 200 ng\/µL. It can be used to measure oligonucleotides or long ssDNA and is compatible with both a Qubit fluorometer and fluorescence microplate readers.\u003c\/p\u003e\n\n  \u003cp\u003eCommon contaminants, including salts, free nucleotides, solvents, detergents, and proteins, are well tolerated by the assay. Nucleotides and short oligonucleotides of six bases or fewer do not interfere with quantification. The StarLighter ssDNA reagent also exhibits fluorescence enhancement when bound to double-stranded DNA and RNA, which should be considered when evaluating samples containing these nucleic acids.\u003c\/p\u003e\n\u003c\/section\u003e\n\n\u003csection\u003e\n  \u003ch2\u003eSpecification\u003c\/h2\u003e\n\n  \u003cdiv style=\"width:100%; overflow-x:auto; -webkit-overflow-scrolling:touch;\"\u003e\n    \u003ctable style=\"width:100%; min-width:680px; border-collapse:collapse;\"\u003e\n      \u003ctbody\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\"\u003eProduct Name\u003c\/th\u003e\n          \u003ctd\u003eStarLighter ssDNA Assay Kit\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\"\u003eProduct Type\u003c\/th\u003e\n          \u003ctd\u003eFluorescent ssDNA Quantification Kit\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\"\u003eTarget\u003c\/th\u003e\n          \u003ctd\u003eOligonucleotides or long single-stranded DNA\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\"\u003eInitial ssDNA Concentration Range\u003c\/th\u003e\n          \u003ctd\u003e50 pg\/µL–200 ng\/µL, depending on sample volume\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\"\u003eTotal Assay Range\u003c\/th\u003e\n          \u003ctd\u003e1–200 ng\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\"\u003eSensitivity\u003c\/th\u003e\n          \u003ctd\u003e1 ng\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\"\u003eDetection Method\u003c\/th\u003e\n          \u003ctd\u003eFluorescence-based quantification\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\"\u003eCompatible Instruments\u003c\/th\u003e\n          \u003ctd\u003eQubit fluorometer or fluorescence microplate reader\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\"\u003eContaminant Tolerance\u003c\/th\u003e\n          \u003ctd\u003eSalts, free nucleotides, solvents, detergents, and proteins are well tolerated\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\"\u003eShort Oligonucleotide Interference\u003c\/th\u003e\n          \u003ctd\u003eNucleotides and short oligonucleotides of six bases or fewer do not interfere\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\"\u003eOther Nucleic Acid Response\u003c\/th\u003e\n          \u003ctd\u003eThe reagent exhibits fluorescence enhancement when bound to dsDNA and RNA\u003c\/td\u003e\n        \u003c\/tr\u003e\n      \u003c\/tbody\u003e\n    \u003c\/table\u003e\n  \u003c\/div\u003e\n\n  \u003ch3\u003eOrdering Information\u003c\/h3\u003e\n\n  \u003cdiv style=\"width:100%; overflow-x:auto; -webkit-overflow-scrolling:touch;\"\u003e\n    \u003ctable style=\"width:100%; min-width:560px; border-collapse:collapse;\"\u003e\n      \u003cthead\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"col\"\u003eDescription\u003c\/th\u003e\n          \u003cth scope=\"col\"\u003eCatalog Number\u003c\/th\u003e\n          \u003cth scope=\"col\"\u003eSize\u003c\/th\u003e\n        \u003c\/tr\u003e\n      \u003c\/thead\u003e\n      \u003ctbody\u003e\n        \u003ctr\u003e\n          \u003ctd\u003eStarLighter ssDNA Assay Kit\u003c\/td\u003e\n          \u003ctd\u003eFS-T1008-V2-S\u003c\/td\u003e\n          \u003ctd\u003e100 assays\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd\u003eStarLighter ssDNA Assay Kit\u003c\/td\u003e\n          \u003ctd\u003eFS-T1008-V2\u003c\/td\u003e\n          \u003ctd\u003e1000 assays\u003c\/td\u003e\n        \u003c\/tr\u003e\n      \u003c\/tbody\u003e\n    \u003c\/table\u003e\n  \u003c\/div\u003e\n\u003c\/section\u003e\n\n\u003csection\u003e\n  \u003ch2\u003eFeatures\u003c\/h2\u003e\n\n  \u003cul\u003e\n    \u003cli\u003eFluorescence-based single-stranded DNA quantification\u003c\/li\u003e\n    \u003cli\u003eTotal ssDNA assay range of 1–200 ng\u003c\/li\u003e\n    \u003cli\u003eInitial ssDNA concentration range from 50 pg\/µL to 200 ng\/µL, depending on sample volume\u003c\/li\u003e\n    \u003cli\u003eDetection of oligonucleotides and long ssDNA\u003c\/li\u003e\n    \u003cli\u003eCompatible with a Qubit fluorometer\u003c\/li\u003e\n    \u003cli\u003eCompatible with fluorescence microplate readers\u003c\/li\u003e\n    \u003cli\u003eHigh sensitivity with a stated sensitivity of 1 ng\u003c\/li\u003e\n    \u003cli\u003eExcellent tolerance to common contaminants\u003c\/li\u003e\n    \u003cli\u003eEasy and fast workflow\u003c\/li\u003e\n    \u003cli\u003eAvailable in 100-assay and 1000-assay configurations\u003c\/li\u003e\n  \u003c\/ul\u003e\n\u003c\/section\u003e\n\n\u003csection\u003e\n  \u003ch2\u003eApplication\u003c\/h2\u003e\n\n  \u003cp\u003eThe StarLighter ssDNA Assay Kit is designed for accurate fluorescent quantification of single-stranded DNA across a total assay range of 1–200 ng. It supports the following applications:\u003c\/p\u003e\n\n  \u003cul\u003e\n    \u003cli\u003eSingle-stranded DNA quantification\u003c\/li\u003e\n    \u003cli\u003eOligonucleotide quantification\u003c\/li\u003e\n    \u003cli\u003eLong ssDNA quantification\u003c\/li\u003e\n    \u003cli\u003eFluorescence-based ssDNA measurement with a Qubit fluorometer\u003c\/li\u003e\n    \u003cli\u003eFluorescence-based ssDNA measurement with a fluorescence microplate reader\u003c\/li\u003e\n  \u003c\/ul\u003e\n\u003c\/section\u003e\n\n\u003csection\u003e\n  \u003ch2\u003eFAQ\u003c\/h2\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat is the quantification range of the StarLighter ssDNA Assay Kit?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe total assay range is 1–200 ng. Depending on sample volume, the assay detects initial ssDNA concentrations from 50 pg\/µL to 200 ng\/µL.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat types of single-stranded DNA can the assay detect?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe assay detects oligonucleotides and long single-stranded DNA.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eCan the ssDNA Assay Kit be used with a Qubit fluorometer?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eYes. The StarLighter ssDNA Assay Kit is compatible with a Qubit fluorometer.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eCan the assay be used with a fluorescence microplate reader?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eYes. The assay is compatible with fluorescence microplate readers.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eDo nucleotides or short oligonucleotides interfere with the assay?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eNucleotides and short oligonucleotides of six bases or fewer do not interfere with the quantification assay.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eDo dsDNA and RNA affect ssDNA quantification?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe StarLighter ssDNA reagent exhibits fluorescence enhancement when bound to double-stranded DNA and RNA. The possible presence of dsDNA or RNA should therefore be considered when evaluating ssDNA samples.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhich contaminants are tolerated by the assay?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eCommon contaminants, including salts, free nucleotides, solvents, detergents, and proteins, are well tolerated by the assay.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhich pack sizes are available?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eCatalog number FS-T1008-V2-S contains 100 assays. Catalog number FS-T1008-V2 contains 1000 assays.\u003c\/p\u003e\n  \u003c\/details\u003e\n\u003c\/section\u003e","brand":"Qihengxing","offers":[{"title":"100 Assays","offer_id":53193987227829,"sku":"FS-T1008-V2-S","price":0.0,"currency_code":"USD","in_stock":false},{"title":"1000 Assays","offer_id":53193987260597,"sku":"FS-T1008-V2","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/starlighter-ssdna-assay-kit.png?v=1788233047","url":"https:\/\/biofargo.com\/products\/ssdna-assay-kit-for-single-stranded-dna-quantification-starlighter","provider":"Biofargo","version":"1.0","type":"link"}