Description

Rat Primary Liver Kupffer Cells from Cell Biologics are isolated from Sprague-Dawley rat liver tissues and grown in tissue culture plates with Cell Biologics’ Cell Culture Medium. Live Cells at passage 0 in culture plates/wells or flasks are shipped and cells are ready used for experiments. Cells are characterized by immunofluorescence staining using an antibody of CD11b. Primary Liver Kupffer Cells can not be cultured indefinitely and can not be passaged.

Handling of Arriving Live Cells

Live Cells at passage 0 in culture plates or flasks are shipped overnight. When you receive the live cells in plates or flasks, remove the sticker from the filter cap, and keep the plates or flasks with 3-15 ml existing medium in a 37°C CO₂ incubator for 1 hour before replacing the desired Cell Biologics' cell culture medium.

Suggested Medium

M3368 Complete Macrophage Medium /w Kit (500 ml)

Documents

RA-6226S

Cell-Culture-Protocol-CB

Disclaimer

Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.

Sprague-Dawley Rat Liver Kupffer Cells