Specification

Package	25 RXNS
Full name	SpeedyCut NsiI
Applications	Fast enzymatic digestion
Summary	SpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.
Features	All SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.
Composition	25 ul enzyme, 10X SpeedyOne Buffer
Recognition Site	5'…-ATGCA↓T…-3' 3'…-T↑ACGTA…-5'
Reaction Buffer	10X SpeedyOne Buffer
Recommended Reaction Temperature	37°C
Unit Definition	At 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.
Methylation Effects	Dam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect; EcoKI: Methylation does not affect; EcoBI: Cleavage may be blocked when sequences overlap.
Thermal Inactivation	Heat at 80 °C for 20 min to lose activity.
Component	25 ul enzyme, 10X SpeedyOne Buffer

Storage

-20°C