Description

In bacteria and archaea CRISPR- Cas systems provide sequence based adaptive immunity against mobile genetic elements such as phages and plasmids (1). Cas9 is a multidomain nuclease from the bacterium Streptococcus pyogenes characteristic of type II CRISPR- Cas systems (2). Using single-guide RNA (sgRNA), Cas9 is targeted to specific genomic loci where it generates a blunt ended double strand break (3). Cas9 can be used for genetic engineering experiments such as genome editing and generating transgenic models (4).

Accession Number

Q99ZW2

Formulation

Recombinant protein stored in 50mM sodium phosphate, pH 7.5, 300mM NaCl, 150mM imidazole, 1mM DTT, 10% glycerol.

Storage

Store product at -70℃. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Always include RNase inhibitor at manufacturer recommended concentrations in Cas enzyme reactions.

Documents

SpCas9-NLS, Active

References

Gong T, et al. 2019. CRISPR-Cas Systems in Streptococci. Curr Issues Mol Biol. 32:1-38.
Nishimasu H, et al. 2014. Crystal structure of Cas9 in complex with guide RNA and target DNA. Cell. 156(5):935-49.
Sapranauskas R, et al. 2011. The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli. Nucleic Acids Res.39(21):9275-82.
Adli M. 2018 The CRISPR tool kit for genome editing and beyond. Nat Commun. 9(1):1911.