Description

This kit provides a quick and easy method for extracting genomic DNA from various sources of soil. There are a large number of inhibitors such as humic acid and metal ions in soil samples. Even if these substances are present in small amounts in the purified DNA, they will have an impact on downstream reactions, such as PCR and restriction enzyme digestion. Therefore, the key to purifying soil DNA is how to effectively remove inhibitors in the soil.

This kit uses DNA-Only Column that can specifically bind to DNA, a brand-new Foregene Protease and a unique buffer system, which can effectively remove various inhibitors from the soil without organic solvent extraction or ethanol and isopropanol precipitation. The extraction of DNA from soil samples can be completed in 90 minutes.

Features

No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove RNA from genomic DNA without adding RNase during the experiment, avoiding the laboratory from being contaminated by exogenous RNase.
Fast speed: The operation is simple, and the soil genomic DNA extraction operation can be completed within 90 minutes.
Convenient: The centrifugation is performed at room temperature, and there is no need for 4°C low-temperature centrifugation or ethanol precipitation of DNA.
Safety: No organic reagent extraction is required.
High quality: The extracted genomic DNA has large fragments, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.

Application

Kit Application:

It is suitable for the purification of genomic DNA from the following samples: silt samples, flower bed soil, treading soil, forest soil, wasteland soil, pond soil, lakeside soil, lawn soil and other samples.

Genomic DNA Appliaction:

The genomic DNA purified by Soil DNA Isolation Kit has high purity and can be used for routine molecular biology operations, such as: restriction enzyme digestion, PCR, Southern blotting, library construction and other experiments.

Work Flow

Storage

This kit can be stored for 12 months under dry conditions at room temperature (15-25°C), if it needs to be stored for a longer period of time, it can be stored at 2-8°C.

Note: If stored at low temperature, the solution is prone to precipitation. Before use, be sure to place the solution in the kit at room temperature for a period of time. If necessary, preheat it in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix it before use.
Foregene Protease solution has a unique formula, which is active when stored at room temperature for a long time (3 months); its activity and stability will be better when stored at 4°C, so it is recommended to store it at 4°C, remember not to keep it at -20°C.
Dry Lysozyme powder is stored at -20°C; the prepared Lysozyme solution is divided into small portions and stored at -20°C.

Documents

Soil DNA Isolation Kit Instruction Manual-V1.0-EN