Description

Laboratories running real-time PCR experiments with up to 48 reactions require a system that supports common PCR consumables, multiple fluorescence channels, stable temperature control, and different data-analysis modes. The SLAN®-48P is a 48-well real-time PCR system with a reaction volume range of 15–100 µL. It is compatible with 0.2 mL PCR tubes, 8-tube strips, and 48-well PCR plates.

For multiplex real-time PCR workflows, the SLAN®-48P provides four standard fluorescence channels and supports two additional optional channels. When configured with six fluorescence channels, the system supports six-target fluorescence detection without cross-interference correction. Compatible probes and dyes include FAM, SYBR Green, VIC, HEX, JOE, TET, TAMRA, Cy3, ROX, Texas Red, CY5, Quasar 705, and Atto 425.

The thermal system provides a temperature control range of 4–99°C, a maximum heating and cooling rate of 4.0°C/s, temperature uniformity of ±0.1°C, and temperature accuracy of ±0.1°C. Its optical detection system uses a maintenance-free LED excitation source and a high-sensitivity photoelectric sensor.

The SLAN®-48P also supports high-resolution melting analysis. The software provides qualitative analysis, absolute quantification, relative quantification, standard melting curves, endpoint allele genotyping, melting-curve genotyping, high-resolution melting analysis, and isothermal amplification. Experimental data can be exported in XLSX, CSV, and TXT formats.

Specification

Product Name	SLAN®-48P Real-Time PCR System
Model	SLAN®-48P
Sample Capacity	48 wells
Reaction Volume	15–100 µL
Compatible Consumables	0.2 mL PCR tubes, 8-tube strips, and 48-well PCR plates
Detection Sensitivity	Minimum resolution of 1 copy
Linear Range	10⁰–10¹⁰ copies
Repeatability	CV <1.00%
Sample Linearity	\|r\| ≥0.9990
Internal Reference Dye Correction	Not required
Multiplex Fluorescence Detection	Up to six-target detection without cross-interference correction
HRM Analysis	Supported
Temperature Control Method	Semiconductor method
Temperature Control Modes	Block control and tube control
Temperature Range	4–99°C
Maximum Heating and Cooling Rate	4.0°C/s
Temperature Uniformity	±0.1°C
Temperature Accuracy	±0.1°C
Heated Lid	Automatic heated lid
Excitation Source	Maintenance-free LED
Detector	High-sensitivity photoelectric sensor
Data Export Formats	XLSX, CSV, and TXT
Custom Reports	Supported
Operating System	Microsoft Windows XP, Vista, 7, and 8
Minimum Computer Memory	4 GB
Minimum Hard Drive Capacity	40 GB
Power Input	220 VAC, 50 Hz
Maximum Power	500 VA
Interfaces	RS232 and USB
Weight	16 kg

Fluorescence Channels

Channel	Compatible Probes and Dyes	Excitation Wavelength	Detection Wavelength	Configuration
Channel 1	FAM / SYBR Green	470 nm	510 nm	Standard
Channel 2	VIC / HEX / JOE / TET / TAMRA / Cy3	530 nm	565 nm	Standard
Channel 3	ROX / Texas Red	585 nm	620 nm	Standard
Channel 4	CY5	630 nm	665 nm	Standard
Channel 5	Quasar 705	690 nm	705 nm	Optional
Channel 6	Atto 425	420 nm	470 nm	Optional

Features

48-Well Reaction Capacity

The SLAN®-48P provides a 48-well reaction platform for real-time fluorescence PCR analysis.

Flexible PCR Consumable Compatibility

The 48-well qPCR instrument accepts 0.2 mL PCR tubes, 8-tube strips, and 48-well PCR plates. The supported reaction volume is 15–100 µL.

Up to Six Fluorescence Channels

The system provides four standard fluorescence channels. Channel 5 and Channel 6 are optional, allowing the SLAN®-48P to support up to six fluorescence channels.

Six-Target Multiplex Detection

The six-channel configuration supports six-target fluorescence detection without cross-interference correction.

High-Resolution Melting Analysis

The SLAN®-48P supports high-resolution melting curve analysis as part of its software analysis functions.

Precise Temperature Control

The thermal system provides a 4–99°C temperature range, a maximum heating and cooling rate of 4.0°C/s, temperature uniformity of ±0.1°C, and temperature accuracy of ±0.1°C.

Automatic Heated Lid

The SLAN®-48P is equipped with an automatic heated lid.

Maintenance-Free LED Excitation

The optical system uses a maintenance-free LED excitation source and a high-sensitivity photoelectric sensor.

No Internal Reference Dye Correction

The manufacturer states that internal reference dye correction is not required.

Multiple Analysis Modes

The software supports qualitative analysis, absolute quantification, relative quantification, standard melting curves, endpoint allele genotyping, melting-curve genotyping, high-resolution melting analysis, and isothermal amplification.

Data Export and Reporting

Experimental data can be exported in XLSX, CSV, and TXT formats. Custom reports are supported.

Application

Qualitative real-time PCR analysis
Absolute quantitative PCR analysis
Relative quantitative PCR analysis
Standard melting curve analysis
Endpoint allele genotyping
Melting-curve genotyping
High-resolution melting curve analysis
Isothermal amplification
Multicolor fluorescence detection
Multiplex real-time PCR analysis

FAQ

What is the sample capacity of the SLAN®-48P real-time PCR system?

The SLAN®-48P has a sample capacity of 48 wells.

What reaction volume does the SLAN®-48P support?

The supported reaction volume is 15–100 µL.

Which PCR consumables are compatible with the SLAN®-48P?

The system is compatible with 0.2 mL PCR tubes, 8-tube strips, and 48-well PCR plates.

How many fluorescence channels does the SLAN®-48P support?

The system includes four standard fluorescence channels. Channel 5 for Quasar 705 and Channel 6 for Atto 425 are optional.

Which fluorescent probes and dyes are supported?

The listed probes and dyes are FAM, SYBR Green, VIC, HEX, JOE, TET, TAMRA, Cy3, ROX, Texas Red, CY5, Quasar 705, and Atto 425. Quasar 705 and Atto 425 require the optional fifth and sixth channels.

Does the SLAN®-48P support multiplex real-time PCR?

Yes. The system supports up to six-target fluorescence detection without cross-interference correction when configured with six fluorescence channels.

Does the SLAN®-48P support HRM analysis?

Yes. High-resolution melting curve analysis is included among the listed software analysis functions.

Does the SLAN®-48P require internal reference dye correction?

No. The manufacturer states that internal reference dye correction is not required.

What is the detection sensitivity of the SLAN®-48P?

The stated minimum resolution is 1 copy. The stated linear range is 10⁰–10¹⁰ copies, repeatability is CV <1.00%, and sample linearity is |r| ≥0.9990.

What are the temperature performance specifications?

The temperature range is 4–99°C. The maximum heating and cooling rate is 4.0°C/s, temperature uniformity is ±0.1°C, and temperature accuracy is ±0.1°C.

Which analysis functions are available?

The software supports qualitative analysis, absolute quantification, relative quantification, standard melting curves, endpoint allele genotyping, melting-curve genotyping, HRM analysis, and isothermal amplification.

Which data export formats are supported?

Experimental data can be exported in XLSX, CSV, and TXT formats. Custom reports are also supported.

SLAN®-48P 48-Well Real-Time PCR System