{"product_id":"skygen-stagonospora-sacchari-probe-realtime-pcr-kit-without-internal-control","title":"Stagonospora sacchari Probe Realtime PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eStagonospora sacchari Probe Realtime PCR Kit is a PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified DNA is directly amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-4430 and is now updated to BF-15044956 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n  \u003cli data-end=\"191\" data-start=\"127\"\u003e\u003cp data-end=\"124\" data-start=\"62\"\u003eOne-step PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eCompatible with commonly used DNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2×Probe qPCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Powder\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/μL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eFrequently Asked Questions(FAQs)\u003c\/h2\u003e\r\n\u003cp\u003eMeet our leadership team, dedicated to bringing Biofargo's vision to life while supporting our customers, partners, and employees with excellence and integrity.\u003c\/p\u003e\r\n\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-4430.pdf\"\u003e\u003cstrong\u003eStagonospora sacchari Probe Realtime PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-4430.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282335752373,"sku":"BF-15044956","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_d802fda4-3499-4029-82f8-3b542ca4845a.jpg?v=1774197103","url":"https:\/\/biofargo.com\/products\/skygen-stagonospora-sacchari-probe-realtime-pcr-kit-without-internal-control","provider":"Biofargo","version":"1.0","type":"link"}