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Description
Sf9 HCP ELISA Kit is based on the solid-phase enzyme-linked immunosorbent assay (ELISA) with a double-antibody sandwich technique to detect residual host cell proteins (HCPs) from Sf9 cells. A sheep polyclonal antibody specific to Sf9 HCPs was employed in the assay to capture any remaining HCPs in the sample. The antibody coverage is assessed by the current mainstream method. Both the Calibration standards (or test sample) and the HRP (Horseradish Peroxidase) labeled with anti-Sf9 HCP antibody were simultaneously added to the microtiter plate coated with the affinity purified capture antibody, and followed by incubation and washing. Then TMB (3,3',5,5' -tetramethylbenzidine) substrate was added into reaction, HRP catalyzed the oxidation of TMB by H2O2 to produce a blue colored product (maximum absorption peak at 655 nm). Then the stop solution was added to terminate the enzymatic reaction, resulting in a yellow colored product (maximum absorption peak at 450 nm). The absorbance values at 450 nm wavelength was positively correlated with the HCPs concentration in the calibration standard and the samples. The concentration of HCPs in the samples can be calculated using a dose-response curve.
| Components | Shipping Condition | Unit Size | Detection Method |
| Sf9 HCP Calibration Standard | 2-8℃ | 96 Tests | Sandwich ELISA |
| Anti-Sf9 HCP Microtiter Strips | |||
| Reconstitution Solution | |||
| Diluent | |||
| Wash Buffer Concentrate (10×) | |||
| Anti-Sf9:HRP (100×) | |||
| TMB Substrate | |||
| Stop Solution | |||
| Sealing Film |
Specifications
| Linearity & Range | 3-243 ng/mL, R2>0.990 |
| Accuracy | Recovery Rate= 88.9%-100.6% |
| LLOQ | 3 ng/mL |
| ULOQ | 243 ng/mL |
| LOD | 0.9 ng/mL |
| Precision | 3.2%-7.7% (CV≤20%) |
| Antibody Coverage (IMBS-2D) | 70.4%-90.6% |
| Antibody Coverage (IMBS-LC/MS) | 91.2% (Unique Peptide ≥2) |
| Specificity | No cross-reactivity with MDCK, Vero, HEK293T, CHO. E.coli and P.pastoris strains. |
| Robustness | Incubate at 25±5℃, CV≤ 20% |
| Instrument suitability not limited to Thermo Multiskan FC, MD Spectra Max ABS Microplate Readers |
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When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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