{"product_id":"sds-protein-extraction-buffer","title":"SDS Lysis Buffer","description":"\u003c!-- =========================\nPRODUCT DESCRIPTION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eSDS Lysis Buffer is a strong denaturing protein extraction buffer designed for efficient cell and tissue lysis when mild lysis reagents cannot fully release target proteins. During protein sample preparation, incomplete disruption of cellular structures, poor solubilization of membrane-associated proteins, or insufficient extraction efficiency from complex biological samples can affect downstream protein analysis. This SDS-based cell lysis buffer provides powerful protein solubilization capability to generate reliable protein lysates for Western blot, SDS-PAGE, and other protein analysis workflows.\u003c\/p\u003e\n\u003cp\u003eFormulated with SDS detergent, Tris buffer, EDTA, and protein protection components, SDS Lysis Buffer enables rapid disruption of cellular membranes and efficient extraction of total proteins from animal cells, plant samples, bacteria, yeast, and tissue specimens. Compared with mild non-denaturing lysis buffers, this strong protein extraction buffer provides enhanced recovery of difficult-to-solubilize proteins and is particularly suitable for applications requiring complete protein denaturation and maximum protein yield.\u003c\/p\u003e\n\u003cp\u003eSDS Lysis Buffer is optimized for preparing protein lysates for electrophoretic and antibody-based detection workflows. Due to the presence of SDS detergent, lysates prepared with this buffer are not suitable for Bradford protein assays and should be quantified using detergent-compatible protein determination methods when required.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nSPECIFICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eSDS Lysis Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eAP0761\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eVolume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eStrong SDS Protein Extraction Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eBuffer Composition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e50 mM Tris (pH 8.1), 1% SDS, EDTA, protease and phosphatase inhibitor components\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eProtein extraction, Western Blot, SDS-PAGE, ELISA, IP, ChIP sample preparation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSample Types\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eAnimal cells, tissues, plants, bacteria, yeast\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e18 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFEATURES\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eStrong protein extraction capability:\u003c\/strong\u003e Provides efficient cell disruption and protein solubilization for samples containing difficult-to-extract proteins.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSDS-based denaturing lysis system:\u003c\/strong\u003e Uses SDS detergent to disrupt cellular membranes and promote complete protein extraction.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eEfficient cell and tissue lysis:\u003c\/strong\u003e Suitable for preparation of whole-cell lysates and tissue protein extracts from diverse biological samples.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eEnhanced recovery of membrane-associated proteins:\u003c\/strong\u003e Supports extraction of proteins tightly associated with cellular structures.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein degradation protection:\u003c\/strong\u003e Contains EDTA and inhibitor components to help maintain protein integrity during sample preparation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCompatible with electrophoresis workflows:\u003c\/strong\u003e Ideal for SDS-PAGE and Western blot protein sample preparation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nAPPLICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eSDS Lysis Buffer is widely used as a strong protein extraction reagent in molecular biology, biochemical research, and protein analysis workflows where efficient solubilization and high protein recovery are required.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eWestern Blot Protein Extraction:\u003c\/strong\u003e Generates high-quality protein lysates for SDS-PAGE separation and Western blot detection of target proteins.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSDS-PAGE Sample Preparation:\u003c\/strong\u003e Provides denatured protein samples suitable for electrophoretic protein separation and analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTotal Protein Extraction:\u003c\/strong\u003e Enables efficient extraction of total cellular proteins from cultured cells and biological samples.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCell Protein Extraction:\u003c\/strong\u003e Suitable for adherent cells and suspension cells requiring strong lysis conditions.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTissue Protein Extraction:\u003c\/strong\u003e Supports extraction of proteins from animal and plant tissues where stronger disruption is required.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eMembrane Protein Extraction:\u003c\/strong\u003e Helps improve solubilization of membrane-associated and difficult-to-extract proteins.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBacterial and Yeast Protein Extraction:\u003c\/strong\u003e Can be used for microbial protein preparation with optional enzymatic pretreatment when additional disruption is required.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eChIP Sample Preparation:\u003c\/strong\u003e Suitable for chromatin-related sample preparation workflows when SDS-compatible downstream processing is applied.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eELISA and Protein Analysis:\u003c\/strong\u003e Provides prepared protein lysates for selected immunoassay and biochemical analysis workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPROTOCOL OVERVIEW\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eProtocol Overview\u003c\/h2\u003e\n\u003cp\u003eFor optimal protein extraction performance, samples should be processed under cold conditions to minimize protein degradation.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eAdd PMSF or other required protease\/phosphatase inhibitors before use according to experimental requirements.\u003c\/li\u003e\n\u003cli\u003eLyse samples on ice or at 4 °C to maintain protein stability.\u003c\/li\u003e\n\u003cli\u003eAdd SDS Lysis Buffer to cells or tissues and ensure complete sample disruption.\u003c\/li\u003e\n\u003cli\u003eCentrifuge lysates at 10,000–14,000 ×g for 3–5 minutes and collect the clarified supernatant for downstream analysis.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eBecause SDS-containing lysates contain high detergent concentrations, Bradford protein assays are not recommended. Detergent-compatible protein quantification methods should be selected when protein concentration measurement is required.\u003c\/p\u003e\n\u003ch2\u003eOrdering Information\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eAP0761\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eSDS Lysis Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFAQ\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is SDS Lysis Buffer used for?\u003c\/summary\u003e\n\u003cp\u003eSDS Lysis Buffer is a strong cell lysis and protein extraction reagent used to prepare protein lysates from cells, tissues, bacteria, yeast, and plant samples. It is commonly used for Western blot, SDS-PAGE, and total protein extraction workflows.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs SDS Lysis Buffer suitable for Western blot?\u003c\/summary\u003e\n\u003cp\u003eYes. SDS Lysis Buffer is especially suitable for Western blot sample preparation because SDS efficiently solubilizes proteins and produces denatured protein lysates compatible with SDS-PAGE analysis.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the difference between SDS Lysis Buffer and RIPA Buffer?\u003c\/summary\u003e\n\u003cp\u003eSDS Lysis Buffer provides stronger denaturing extraction through SDS detergent and is designed for maximum protein solubilization. RIPA buffers are generally milder and are often selected when partial preservation of protein complexes or native protein structures is required.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan SDS Lysis Buffer be used for protein interaction studies?\u003c\/summary\u003e\n\u003cp\u003eSDS Lysis Buffer is a strong denaturing lysis reagent and may disrupt many protein-protein interactions. For native protein interaction studies such as Co-IP, milder non-denaturing buffers are generally preferred.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan Bradford assay be used after SDS Lysis Buffer extraction?\u003c\/summary\u003e\n\u003cp\u003eNo. SDS detergent can interfere with Bradford protein quantification and may result in inaccurate measurements. Detergent-compatible protein assays should be considered instead.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat samples can be extracted using SDS Lysis Buffer?\u003c\/summary\u003e\n\u003cp\u003eSDS Lysis Buffer can be used for animal cells, tissue samples, plant materials, bacteria, and yeast. Sample disruption conditions may require optimization depending on sample characteristics.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPRODUCT SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!-- =========================\nFAQ SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AccuRef Scientific","offers":[{"title":"100mL","offer_id":52776280228021,"sku":"AP0761","price":61.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/5_84f27dda-71d8-4426-b2d9-07b6069991e8.jpg?v=1783488898","url":"https:\/\/biofargo.com\/products\/sds-protein-extraction-buffer","provider":"Biofargo","version":"1.0","type":"link"}