Specification

Full name	RUNX1/RUNX2/RUNX3 rabbit monoclonal antibody
Alternative names	50 μl/100 μl
Reactivity	rabbit monoclonal
Applications	WB, IHC, IF
Host	Rabbit
Clone type	rabbit monoclonal
Target Background	Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by the RUNX1 gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene. The RUNX2 gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Two regions of potential trinucleotide repeat expansions are present in the N-terminal region of the encoded protein, and these and other mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. The RUNX3 gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Alternative splicing results in multiple transcript variants.
Swissprot No	Q01196/Q13950/Q13761
Gene Accession	861/860/864
WB Predicted band size	49 kDa
WB Positive control
WB Recommended dilution	500-2000
IHC predicted cell location	Predicted cell location: Nucleus
IHC positive control	Positive control: mouse colon
IHC Recommed dilution	Recommended dilution: 50-100

Storage

-20°C, 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA. Avoid repeated freeze-thaw cycles.

WB PIC Description

P291371-WB-1-P

IHC PIC Description

Immunohistochemical analysis of paraffin-embedded mouse colon tissue using P291371(RUNX1/RUNX2/RUNX3 Antibody) at dilution 1/50

RUNX1/RUNX2/RUNX3 rabbit monoclonal antibody