Descprition

FOREGENE’s 2× RT EasyTM Mix can synthesize the first-strand cDNA at a temperature as high as 50°C, which is conducive to the reverse transcription reaction of complex secondary structure RNA templates.

The RNA purified in the laboratory often contains alcohol and guanidine salt residues, which have strong inhibitory properties on most reverse transcriptases, resulting in unsatisfactory reverse transcription effects or low reverse transcription efficiency. FOREGENE 2× RT EasyTM Mix shows extremely high tolerance to alcohol and guanidine salt, the highest tolerance to alcohol in RNA samples is 45%, and the highest tolerance to guanidine salt is 750mM. Even if the RNA is not pure, the reverse transcription reaction can be performed with FORREGENE 2×RT Mix. The unique reaction system makes the RT reaction easier, faster and more efficient, and the synthesis of the first strand cDNA can be completed in 25 minutes.

This product can be used in conjunction with Foregene's fluorescent quantitative product Real Time PCR EasyTM to obtain high-quality experimental results.

Product components

Kit contents	RT-01011	RT-01012
Kit contents	25 times (20μl system)	100 times (20μl system)
2× RT EasyTM Mix	0.25ml	1ml
Random Primer (50μM)	50μl	200μl
Oligo(dT)18 Primer (50μM)	50μl	200μl
RNase-Free ddH2O	1.7ml	1.7ml
Instructions Manual	1 piece	1 piece

Features

Efficient reverse transcription system, it only takes 25 minutes to complete the synthesis of the first strand cDNA.
High-sensitivity reverse transcription system, pg-level templates can also get high-quality cDNA.
The reverse transcription system has high thermal stability, the reaction temperature of the system can be as high as 50 ℃, and it has good reverse transcription performance.
Even impure RNA samples (alcohol up to 45%, guanidine salt up to 750mM) can be subjected to reverse transcription reaction.

Application

Conventional RT-PCR
Synthesis of cDNA for cloning and expression research
Analysis of the primer extension method of the transcription initiation site
RACE (rapid amplification of cDNA ends)
Linear RNA amplification
Chip mark

Preparations before operation

It is strongly recommended that users read the instructions carefully before using this kit. The RT Easy series kits are simple, convenient and fast to operate. The instructions provide the correct use of the entire kit. Please prepare necessary experimental materials and equipment before use.

Storage and Shelf life

RT EasyTM I is stored at -20°C. Store the product in a constant temperature refrigerator at -20°C immediately after receipt. If the storage conditions are appropriate, the product will not degrade any performance during the 1-year validity period.
The whole process of low-temperature ice box transportation, to ensure that the kit is in a state of <4 ℃.

Documents

RT Easy™ I Instruction Manual-V1.0-EN

RT EasyTM I (Master Premix for first-strand cDNA synthesis)