Description

The RNAsimple Total RNA Kit adopts a new RNA extraction method based on guanidinium isothiocyanate/phenol method. The Buffer RZ specifically designed by TIANGEN can remove genomic DNA and proteins from cells rapidly and efficiently, making the obtained RNA highly pure and stable.

This kit is used to separate total RNA from blood, animal cells, tissues, and plant tissues. Each spin column can process 50-100 mg tissue or 5×106 cells at a time and can process a large number of different samples simultaneously. The reaction can be completed in less than one hour, and the total RNA extracted has a higher yield, better purity, with no DNA and protein contamination, and can be used in various downstream experiments.

Storage

Buffer RZ should be stored protected from light at 2-8°C for 15 months;
others stored at room temperature (15-30°C) for 15 months.

Required Reagents

Chloroform, Ethanol (96% -100%)

Features

The high-purity ready-to-use RNA is suitable for sensitive downstream applications.
Wide applications: The purified RNA can be applied to various experimental samples.
The experiment can be completed in 1 hour with simple operations.

Applications

RT-PCR
Northern Blot, Dot Blot.
Real-Time PCR
PolyA Screening, in vitro translation, RNase protection analysis, molecular cloning.

Experimental Example

Material: 20 mg rat spleen tissue

Method: The total RNA of rat spleen tissue was isolated using the RNAsimple Total RNA Kit.

Results: Please see the above agarose gel electrophoresis picture. 2-4 μl of 100 μl eluates were loaded per lane. The electrophoresis was conducted at 6 V/cm for 30 min on a 1% agarose.

RNAsimple Total RNA Kit For the High-efficient Total RNA Extraction Using The Widely Used Centrifugal Column DP419 (50 preps)-Tiangen