Description

The RNAprep Pure Cell/Bacteria Kit provides a fast, simple and cost-effective method for purification of total RNA from cultured cells and bacteria samples by using effective spin column and unique buffer system. The kit includes RNase-Free Spin Column CR3 for purifying high quality RNA by using silica-membrane technology. High-quality total RNA could be obtained in 30-40 minutes with high-purity and is free of protein and genomic DNA contamination.

Features

Optimized buffers and protocols for cultured cells and bacteria samples make the process simple and convenient.
Unique DNase I minimizes genomic DNA contamination.
Unique RNase-Free Filtration Columns CS eliminates other contaminations.
The high-purity and ready-to-use RNA is suitable for sensitive downstream applications.
No phenol/chloroform extraction, no LiCl and ethanol precipitation, no CsCl gradient centrifugation are needed, which makes the process safe and reliable.

Required Reagents

β-mercaptoethanol, ethanol, lysozyme (optional for bacterial RNA extraction)

Applications

RT-PCR.
Northern Blot, Dot Blot.
Real-Time PCR.
Chip analysis.
PolyA Screening, in vitro translation, RNase protection analysis and molecular cloning.

Storage

DNase I, Buffer RDD, RNase-Free ddH2O (Tubular) should be stored at 2-8℃

Other reagents could be stored at room temperature (15-25℃)

Documents

GDP430-RNAprep Pure CellBacteria Kit-240514