{"product_id":"rna-hs-assay-kit","title":"RNA HS Assay Kit","description":"\u003cstyle\u003e\n  .bf-rich .josh-faq {\n    background: #fff;\n    color: #000;\n    font-family: \"IBM Plex Sans\", sans-serif;\n  }\n\n  .bf-rich .josh-faq__inner { padding: 30px 300px 60px 30px; }\n  .bf-rich .josh-faq__item { border-bottom: 1px solid #e5e7eb; padding: 0; }\n  .bf-rich .josh-faq__item:first-child { border-top: 1px solid #e5e7eb; }\n\n  .bf-rich .josh-faq__question {\n    width: 100%;\n    margin: 0;\n    padding: 20px 0;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    color: #10213d;\n    background: none;\n    border: 0;\n    font: 700 16px\/1.4 \"IBM Plex Sans\", sans-serif;\n    text-align: left;\n    cursor: pointer;\n    list-style: none;\n  }\n\n  .bf-rich .josh-faq__question::-webkit-details-marker { display: none; }\n  .bf-rich .josh-faq__question:hover { color: #055da9; }\n\n  .bf-rich .josh-faq__icon {\n    position: relative;\n    width: 20px;\n    height: 20px;\n    flex: 0 0 20px;\n    transition: transform .25s ease;\n  }\n\n  .bf-rich .josh-faq__icon::before,\n  .bf-rich .josh-faq__icon::after {\n    position: absolute;\n    content: \"\";\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity .25s ease, transform .25s ease;\n  }\n\n  .bf-rich .josh-faq__icon::before {\n    top: 50%;\n    left: 0;\n    width: 100%;\n    height: 2px;\n    transform: translateY(-50%);\n  }\n\n  .bf-rich .josh-faq__icon::after {\n    top: 0;\n    left: 50%;\n    width: 2px;\n    height: 100%;\n    transform: translateX(-50%);\n  }\n\n  .bf-rich .josh-faq__item[open] .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .bf-rich .josh-faq__answer {\n    max-width: 860px;\n    padding: 0 0 20px;\n    color: #333;\n    font: 400 16px\/1.6 \"IBM Plex Sans\", sans-serif;\n  }\n\n  .bf-rich .josh-faq__answer p { margin: 0 0 15px; color: #333; font: inherit; }\n  .bf-rich .josh-faq__answer p:last-child { margin-bottom: 0; }\n  .bf-rich .josh-faq__answer ul { margin: 8px 0 12px; padding-left: 22px; }\n  .bf-rich .josh-faq__answer li { margin-bottom: 6px; font: inherit; list-style-type: disc; }\n\n  @media (max-width: 767px) {\n    .bf-rich .josh-faq__inner { padding: 24px 0 40px; }\n    .bf-rich .josh-faq__question { padding: 16px 0; font-size: 15px; }\n    .bf-rich .josh-faq__answer { font-size: 15px; }\n  }\n\u003c\/style\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eMeasuring low-concentration RNA by UV A260 can be affected by limited sensitivity and substances present in laboratory samples. The Biofargo RNA HS Assay Kit provides a fluorescence-based method that is highly selective for RNA over double-stranded DNA. The assay tolerates the common contaminants tested in the user guide, including salts, free nucleotides, solvents, detergents, and proteins.\u003c\/p\u003e\n\u003cp\u003eDepending on sample volume, the assay measures initial RNA concentrations from 0.5 to 100 ng\/µL, with a total assay range of 5–100 ng. It accepts 1–20 µL of sample in a final assay volume of 200 µL and can be used with a Qubit fluorometer or a fluorescence microplate reader.\u003c\/p\u003e\n\u003cp\u003eThe assay requires a 2-minute incubation at room temperature protected from light. The fluorescent signal remains stable for up to 3 hours after incubation when protected from light. For research use only; not for diagnostic or therapeutic procedures.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 90%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eParameter\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eSpecification\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eProduct name\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eBiofargo RNA HS Assay Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eTarget\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eRNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eSelectivity\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eHighly selective for RNA over double-stranded DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eDetection method\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eFluorescence\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eInitial RNA concentration\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e0.5–100 ng\/µL, depending on sample volume\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eTotal assay range\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e5–100 ng\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eSample volume\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e1–20 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eFinal assay volume\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e200 µL per tube or well\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eAssay temperature\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e20–25 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eIncubation\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2 minutes, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eCompatible instruments\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eQubit fluorometer or fluorescence microplate reader\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eMicroplate reader settings\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e620 nm excitation; 660 nm emission\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eAvailable formats\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eFS-T1006-S: 100 reactions; FS-T1006: 1,000 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eIntended use\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eResearch use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eMeasures initial RNA concentrations from 0.5 to 100 ng\/µL, depending on sample volume.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHighly selective for RNA over double-stranded DNA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAccepts 1–20 µL of sample in a final assay volume of 200 µL.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eIncludes protocols for a Qubit fluorometer and a fluorescence microplate reader.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eRequires a 2-minute incubation at 20–25 °C, protected from light.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFluorescent signal remains stable for up to 3 hours after incubation when protected from light.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eQubit Fluorometer:\u003c\/strong\u003e Quantify RNA in thin-walled, clear 0.5 mL PCR tubes using the two supplied standards and the RNA High Sensitivity assay setting.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eFluorescence Microplate Reader:\u003c\/strong\u003e Quantify RNA in a black-wall, clear-bottom 96-well microplate using a standard curve, 620 nm excitation, and 660 nm emission.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 90%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFS-T1006-S (100 reactions)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFS-T1006 (1,000 reactions)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eRNA HS Buffer QB\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e20 mL\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e200 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eRNA HS Standard QS0\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e100 µL, 0 ng\/µL\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL, 0 ng\/µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eRNA HS Standard QS1\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e100 µL, 10 ng\/µL\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL (4 × 250 µL), 10 ng\/µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eRNA HS Fluorescent Reagent QF\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e100 µL\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eShip on ice.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eStore RNA HS Standards QS0 and QS1 at −25 to −15 °C.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eStore all other kit components at 2–8 °C.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eProtect the working solution and assay tubes from light during preparation and incubation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eFrequently Asked Questions (FAQs)\u003c\/h2\u003e\n\u003cdiv class=\"josh-faq\"\u003e\n\u003csection class=\"josh-faq__inner\"\u003e\n\u003cdiv class=\"josh-faq__list\"\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\n\u003csummary class=\"josh-faq__question\"\u003e What is the measurement range of the Biofargo RNA HS Assay Kit? \u003cspan class=\"josh-faq__icon\"\u003e\u003c\/span\u003e \u003c\/summary\u003e\n\u003cdiv class=\"josh-faq__answer\"\u003e\n\u003cp\u003eDepending on sample volume, the initial RNA concentration range is 0.5–100 ng\/µL. The total assay range is 5–100 ng.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\n\u003csummary class=\"josh-faq__question\"\u003e Can the kit be used with a Qubit fluorometer? \u003cspan class=\"josh-faq__icon\"\u003e\u003c\/span\u003e \u003c\/summary\u003e\n\u003cdiv class=\"josh-faq__answer\"\u003e\n\u003cp\u003eYes. The user guide provides a Qubit workflow using two RNA standards. On a Qubit 4 Fluorometer, select RNA and then RNA High Sensitivity.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\n\u003csummary class=\"josh-faq__question\"\u003e Can the kit be used with a fluorescence microplate reader? \u003cspan class=\"josh-faq__icon\"\u003e\u003c\/span\u003e \u003c\/summary\u003e\n\u003cdiv class=\"josh-faq__answer\"\u003e\n\u003cp\u003eYes. The user guide provides a 96-well workflow with 620 nm excitation and 660 nm emission.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\n\u003csummary class=\"josh-faq__question\"\u003e How much RNA sample is needed? \u003cspan class=\"josh-faq__icon\"\u003e\u003c\/span\u003e \u003c\/summary\u003e\n\u003cdiv class=\"josh-faq__answer\"\u003e\n\u003cp\u003eAdd 1–20 µL of sample and adjust the working solution so that the final volume is 200 µL per tube or well.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\n\u003csummary class=\"josh-faq__question\"\u003e How should the kit components be stored? \u003cspan class=\"josh-faq__icon\"\u003e\u003c\/span\u003e \u003c\/summary\u003e\n\u003cdiv class=\"josh-faq__answer\"\u003e\n\u003cp\u003eStore standards QS0 and QS1 at −25 to −15 °C. Store all other components at 2–8 °C.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003c\/div\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca rel=\"noopener\" type=\"application\/pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RNA_HS_Assay_Kit-User_guide.pdf?v=1789626826\" target=\"_blank\"\u003e\u003cstrong\u003eProduct User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Qihengxing","offers":[{"title":"100 Reactions","offer_id":53193265774773,"sku":"FS-T1006-S","price":39.0,"currency_code":"USD","in_stock":true},{"title":"1000 Reactions","offer_id":53193265807541,"sku":"FS-T1006","price":399.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/exec-2e22d3e5-f051-4a3c-a5a5-fba8fe4fd5d3.png?v=1789627446","url":"https:\/\/biofargo.com\/products\/rna-hs-assay-kit","provider":"Biofargo","version":"1.0","type":"link"}