{"product_id":"rna-br-assay-kit-for-broad-range-rna-quantification-starlighter","title":"RNA BR Assay Kit for Broad Range RNA Quantification——StarLighter","description":"\u003csection\u003e\n  \u003ch2\u003eDescription\u003c\/h2\u003e\n  \u003cp\u003eLaboratories measuring RNA across a broad concentration range need an assay that selectively detects RNA while accommodating different sample volumes and common laboratory contaminants. The StarLighter RNA BR Assay Kit provides a fluorescence-based method for broad range RNA quantification. It is highly selective for RNA over double-stranded DNA (dsDNA) and detects initial RNA concentrations from 1 ng\/µL to 100 ng\/µL, depending on sample volume, with a total assay range of 20–1000 ng.\u003c\/p\u003e\n\n  \u003cp\u003eThis RNA Broad Range Assay Kit accepts 1–20 µL of sample in a final assay volume of 200 µL. The protocol can be performed with either a Qubit fluorometer or a fluorescence microplate reader. For microplate-based RNA quantification, fluorescence is measured at 620 nm excitation and 660 nm emission.\u003c\/p\u003e\n\n  \u003cp\u003eThe assay tolerates common contaminants, including salts, free nucleotides, solvents, detergents, proteins, agarose, and PEG at the concentrations listed below. Signal development requires a 2-minute incubation at room temperature while protected from light. When protected from light, the fluorescent signal remains stable for up to 3 hours after incubation.\u003c\/p\u003e\n\u003c\/section\u003e\n\n\u003csection\u003e\n  \u003ch2\u003eSpecification\u003c\/h2\u003e\n\n  \u003cdiv style=\"width:100%; overflow-x:auto; -webkit-overflow-scrolling:touch;\"\u003e\n    \u003ctable style=\"width:100%; min-width:680px; border-collapse:collapse;\"\u003e\n      \u003ctbody\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\"\u003eProduct Name\u003c\/th\u003e\n          \u003ctd\u003eStarLighter RNA BR Assay Kit\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\"\u003eProduct Type\u003c\/th\u003e\n          \u003ctd\u003eRNA Broad Range Assay Kit\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\"\u003eCatalog Numbers\u003c\/th\u003e\n          \u003ctd\u003eFS-T1007-S; FS-T1007\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\"\u003eInitial RNA Concentration Range\u003c\/th\u003e\n          \u003ctd\u003e1–100 ng\/µL, depending on sample volume\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\"\u003eTotal Assay Range\u003c\/th\u003e\n          \u003ctd\u003e20–1000 ng\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\"\u003eSample Volume\u003c\/th\u003e\n          \u003ctd\u003e1–20 µL\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\"\u003eFinal Assay Volume\u003c\/th\u003e\n          \u003ctd\u003e200 µL\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\"\u003eSelectivity\u003c\/th\u003e\n          \u003ctd\u003eHighly selective for RNA over double-stranded DNA (dsDNA)\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\"\u003eCompatible Instruments\u003c\/th\u003e\n          \u003ctd\u003eQubit fluorometer or fluorescence microplate reader\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\"\u003eMicroplate Reader Format\u003c\/th\u003e\n          \u003ctd\u003e96-well format\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\"\u003eExcitation\u003c\/th\u003e\n          \u003ctd\u003e620 nm\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\"\u003eEmission\u003c\/th\u003e\n          \u003ctd\u003e660 nm\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\"\u003eAssay Temperature\u003c\/th\u003e\n          \u003ctd\u003eRoom temperature, 20–25°C\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\"\u003eIncubation\u003c\/th\u003e\n          \u003ctd\u003e2 minutes at room temperature, protected from light\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\"\u003eSignal Stability\u003c\/th\u003e\n          \u003ctd\u003eUp to 3 hours after incubation when protected from light\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\"\u003eShipping Condition\u003c\/th\u003e\n          \u003ctd\u003eShip on ice\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\"\u003eStandards Storage\u003c\/th\u003e\n          \u003ctd\u003eStore QS0 and QS1 at -25 to -15°C\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\"\u003eOther Components Storage\u003c\/th\u003e\n          \u003ctd\u003eStore at 2–8°C\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\"\u003eIntended Use\u003c\/th\u003e\n          \u003ctd\u003eFor Research Use Only. Not for use in diagnostic or therapeutic procedures.\u003c\/td\u003e\n        \u003c\/tr\u003e\n      \u003c\/tbody\u003e\n    \u003c\/table\u003e\n  \u003c\/div\u003e\n\n  \u003ch3\u003eKit Components\u003c\/h3\u003e\n\n  \u003cdiv style=\"width:100%; overflow-x:auto; -webkit-overflow-scrolling:touch;\"\u003e\n    \u003ctable style=\"width:100%; min-width:760px; border-collapse:collapse;\"\u003e\n      \u003cthead\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"col\"\u003eComponent\u003c\/th\u003e\n          \u003cth scope=\"col\"\u003eFS-T1007-S (100 Reactions)\u003c\/th\u003e\n          \u003cth scope=\"col\"\u003eFS-T1007 (1000 Reactions)\u003c\/th\u003e\n        \u003c\/tr\u003e\n      \u003c\/thead\u003e\n      \u003ctbody\u003e\n        \u003ctr\u003e\n          \u003ctd\u003eRNA BR Buffer QB\u003c\/td\u003e\n          \u003ctd\u003e20 mL\u003c\/td\u003e\n          \u003ctd\u003e200 mL\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd\u003eRNA BR Standard QS0\u003c\/td\u003e\n          \u003ctd\u003e100 µL, 0 ng\/µL\u003c\/td\u003e\n          \u003ctd\u003e1 mL, 0 ng\/µL\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd\u003eRNA BR Standard QS1\u003c\/td\u003e\n          \u003ctd\u003e100 µL, 100 ng\/µL\u003c\/td\u003e\n          \u003ctd\u003e1 mL (4 × 250 µL), 100 ng\/µL\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd\u003eRNA BR Fluorescent Reagent QF\u003c\/td\u003e\n          \u003ctd\u003e100 µL\u003c\/td\u003e\n          \u003ctd\u003e1 mL\u003c\/td\u003e\n        \u003c\/tr\u003e\n      \u003c\/tbody\u003e\n    \u003c\/table\u003e\n  \u003c\/div\u003e\n\n  \u003ch3\u003eRequired Materials Not Supplied\u003c\/h3\u003e\n  \u003cul\u003e\n    \u003cli\u003eQubit fluorometer or fluorescence microplate reader\u003c\/li\u003e\n    \u003cli\u003eThin-walled, clear 0.5 mL PCR tubes for the Qubit fluorometer\u003c\/li\u003e\n    \u003cli\u003eBlack 96-well microplate for the microplate reader\u003c\/li\u003e\n    \u003cli\u003eVortex mixer\u003c\/li\u003e\n    \u003cli\u003ePipettes and sterile, RNase-free pipette tips\u003c\/li\u003e\n  \u003c\/ul\u003e\n\n  \u003ch3\u003eContaminant Tolerance\u003c\/h3\u003e\n\n  \u003cdiv style=\"width:100%; overflow-x:auto; -webkit-overflow-scrolling:touch;\"\u003e\n    \u003ctable style=\"width:100%; min-width:780px; border-collapse:collapse;\"\u003e\n      \u003cthead\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"col\"\u003eContaminant\u003c\/th\u003e\n          \u003cth scope=\"col\"\u003eFinal Concentration in Assay\u003c\/th\u003e\n          \u003cth scope=\"col\"\u003eConcentration in 10 µL Sample\u003c\/th\u003e\n          \u003cth scope=\"col\"\u003eResult\u003c\/th\u003e\n        \u003c\/tr\u003e\n      \u003c\/thead\u003e\n      \u003ctbody\u003e\n        \u003ctr\u003e\n\u003ctd\u003eProtein (BSA)\u003c\/td\u003e\n\u003ctd\u003e10 mg\/mL\u003c\/td\u003e\n\u003ctd\u003e200 mg\/mL\u003c\/td\u003e\n\u003ctd\u003eOK\u003c\/td\u003e\n\u003c\/tr\u003e\n        \u003ctr\u003e\n\u003ctd\u003eSodium acetate\u003c\/td\u003e\n\u003ctd\u003e20 mM\u003c\/td\u003e\n\u003ctd\u003e400 mM\u003c\/td\u003e\n\u003ctd\u003eOK\u003c\/td\u003e\n\u003c\/tr\u003e\n        \u003ctr\u003e\n\u003ctd\u003eAmmonium acetate\u003c\/td\u003e\n\u003ctd\u003e20 mM\u003c\/td\u003e\n\u003ctd\u003e400 mM\u003c\/td\u003e\n\u003ctd\u003eOK\u003c\/td\u003e\n\u003c\/tr\u003e\n        \u003ctr\u003e\n\u003ctd\u003eNaCl\u003c\/td\u003e\n\u003ctd\u003e20 mM\u003c\/td\u003e\n\u003ctd\u003e400 mM\u003c\/td\u003e\n\u003ctd\u003eOK\u003c\/td\u003e\n\u003c\/tr\u003e\n        \u003ctr\u003e\n\u003ctd\u003eMgCl\u003csub\u003e2\u003c\/sub\u003e\n\u003c\/td\u003e\n\u003ctd\u003e5 mM\u003c\/td\u003e\n\u003ctd\u003e100 mM\u003c\/td\u003e\n\u003ctd\u003eOK\u003c\/td\u003e\n\u003c\/tr\u003e\n        \u003ctr\u003e\n\u003ctd\u003ePhenol\u003c\/td\u003e\n\u003ctd\u003e0.1%\u003c\/td\u003e\n\u003ctd\u003e2%\u003c\/td\u003e\n\u003ctd\u003eOK\u003c\/td\u003e\n\u003c\/tr\u003e\n        \u003ctr\u003e\n\u003ctd\u003eChloroform\u003c\/td\u003e\n\u003ctd\u003e0.5%\u003c\/td\u003e\n\u003ctd\u003e10%\u003c\/td\u003e\n\u003ctd\u003eOK\u003c\/td\u003e\n\u003c\/tr\u003e\n        \u003ctr\u003e\n\u003ctd\u003eEthanol\u003c\/td\u003e\n\u003ctd\u003e0.5%\u003c\/td\u003e\n\u003ctd\u003e10%\u003c\/td\u003e\n\u003ctd\u003eOK\u003c\/td\u003e\n\u003c\/tr\u003e\n        \u003ctr\u003e\n\u003ctd\u003eSDS\u003c\/td\u003e\n\u003ctd\u003e0.01%\u003c\/td\u003e\n\u003ctd\u003e0.2%\u003c\/td\u003e\n\u003ctd\u003eOK\u003c\/td\u003e\n\u003c\/tr\u003e\n        \u003ctr\u003e\n\u003ctd\u003eTriton X-100\u003c\/td\u003e\n\u003ctd\u003e0.01%\u003c\/td\u003e\n\u003ctd\u003e0.2%\u003c\/td\u003e\n\u003ctd\u003eOK\u003c\/td\u003e\n\u003c\/tr\u003e\n        \u003ctr\u003e\n\u003ctd\u003eAgarose\u003c\/td\u003e\n\u003ctd\u003e0.1%\u003c\/td\u003e\n\u003ctd\u003e2%\u003c\/td\u003e\n\u003ctd\u003eOK\u003c\/td\u003e\n\u003c\/tr\u003e\n        \u003ctr\u003e\n\u003ctd\u003ePEG\u003c\/td\u003e\n\u003ctd\u003e1%\u003c\/td\u003e\n\u003ctd\u003e20%\u003c\/td\u003e\n\u003ctd\u003eOK\u003c\/td\u003e\n\u003c\/tr\u003e\n        \u003ctr\u003e\n\u003ctd\u003edNTPs\u003c\/td\u003e\n\u003ctd\u003e100 µM\u003c\/td\u003e\n\u003ctd\u003e2 mM\u003c\/td\u003e\n\u003ctd\u003eOK\u003c\/td\u003e\n\u003c\/tr\u003e\n      \u003c\/tbody\u003e\n    \u003c\/table\u003e\n  \u003c\/div\u003e\n\n  \u003cp\u003eThe listed sample concentrations are approximate equivalents for a 10 µL sample volume. An “OK” result usually represents less than 10% perturbation under the stated assay conditions.\u003c\/p\u003e\n\u003c\/section\u003e\n\n\u003csection\u003e\n  \u003ch2\u003eFeatures\u003c\/h2\u003e\n  \u003cul\u003e\n    \u003cli\u003eFluorescent RNA quantification across a total assay range of 20–1000 ng\u003c\/li\u003e\n    \u003cli\u003eInitial RNA concentration range of 1–100 ng\/µL, depending on sample volume\u003c\/li\u003e\n    \u003cli\u003eHigh selectivity for RNA over double-stranded DNA\u003c\/li\u003e\n    \u003cli\u003eFlexible sample input from 1–20 µL\u003c\/li\u003e\n    \u003cli\u003eCompatible with a Qubit fluorometer or fluorescence microplate reader\u003c\/li\u003e\n    \u003cli\u003eTwo-minute signal development at room temperature\u003c\/li\u003e\n    \u003cli\u003eFluorescent signal remains stable for up to 3 hours when protected from light\u003c\/li\u003e\n    \u003cli\u003eTolerates the listed salts, solvents, detergents, proteins, agarose, PEG, and dNTPs\u003c\/li\u003e\n    \u003cli\u003eAvailable in 100-reaction and 1000-reaction configurations\u003c\/li\u003e\n  \u003c\/ul\u003e\n\u003c\/section\u003e\n\n\u003csection\u003e\n  \u003ch2\u003eApplication\u003c\/h2\u003e\n  \u003cp\u003eThe StarLighter RNA BR Assay Kit is intended for broad range RNA quantification in research laboratories. It supports fluorescence-based RNA measurement using either individual assay tubes with a Qubit fluorometer or a black 96-well microplate with a fluorescence microplate reader.\u003c\/p\u003e\n\n  \u003cul\u003e\n    \u003cli\u003eBroad range RNA quantification from 20–1000 ng total RNA\u003c\/li\u003e\n    \u003cli\u003eRNA concentration measurement from samples containing 1–100 ng\/µL RNA\u003c\/li\u003e\n    \u003cli\u003eTube-based RNA quantification with a Qubit fluorometer\u003c\/li\u003e\n    \u003cli\u003e96-well fluorescence microplate-based RNA quantification\u003c\/li\u003e\n  \u003c\/ul\u003e\n\n  \u003cp\u003eThis product is for research use only and is not intended for diagnostic or therapeutic procedures.\u003c\/p\u003e\n\u003c\/section\u003e\n\n\u003csection\u003e\n  \u003ch2\u003eFAQ\u003c\/h2\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat is the quantification range of the StarLighter RNA BR Assay Kit?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe assay detects initial RNA concentrations from 1 ng\/µL to 100 ng\/µL, depending on the sample volume. Its total assay range is 20–1000 ng.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eHow much RNA sample is required for each assay?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eEach assay accepts 1–20 µL of RNA sample. The working solution volume must be adjusted so that the final assay volume is exactly 200 µL.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eCan this RNA Broad Range Assay Kit be used with a Qubit fluorometer?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eYes. The protocol is compatible with a Qubit fluorometer. The assay requires two standards, QS0 and QS1, and thin-walled, clear 0.5 mL PCR tubes. On a Qubit 4 Fluorometer, RNA Broad Range is selected as the assay type.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eCan the kit be used with a fluorescence microplate reader?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eYes. The assay supports a 96-well fluorescence microplate reader workflow. Use a black-wall, clear-bottom microplate to minimize cross-talk and set the reader to 620 nm excitation and 660 nm emission.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eHow long should the RNA assay incubate before measurement?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eIncubate the prepared tubes or microplate for 2 minutes at room temperature, 20–25°C, while protected from light. The fluorescent signal remains stable for up to 3 hours after incubation when protected from light.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eIs the assay selective for RNA over DNA?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eYes. The StarLighter RNA BR Assay is highly selective for RNA over double-stranded DNA.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhich StarLighter RNA BR Assay Kit sizes are available?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eCatalog number FS-T1007-S contains materials for 100 reactions. Catalog number FS-T1007 contains materials for 1000 reactions.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eHow should the StarLighter RNA BR Assay Kit be stored?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe kit ships on ice. RNA BR Standards QS0 and QS1 must be stored at -25 to -15°C. All other kit components should be stored at 2–8°C. The fluorescent reagent, working solution, and assay tubes must be protected from light to minimize photobleaching.\u003c\/p\u003e\n  \u003c\/details\u003e\n\u003c\/section\u003e","brand":"Qihengxing","offers":[{"title":"100 Reactions","offer_id":53193546825909,"sku":"FS-T1007-S","price":0.0,"currency_code":"USD","in_stock":false},{"title":"1000 Reactions","offer_id":53193546858677,"sku":"FS-T1007","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/starlighter-rna-br-assay-kit.jpg?v=1788231165","url":"https:\/\/biofargo.com\/products\/rna-br-assay-kit-for-broad-range-rna-quantification-starlighter","provider":"Biofargo","version":"1.0","type":"link"}