{"product_id":"ripa-lysis-buffer-middle","title":"RIPA Lysis Buffer (Mild)","description":"\u003c!-- =========================\nPRODUCT DESCRIPTION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eRIPA Lysis Buffer-middle is a medium-strength RIPA protein extraction buffer designed for routine cell and tissue protein extraction when researchers need a balance between efficient protein solubilization and preservation of protein complexes. During protein sample preparation, insufficient cell disruption may reduce protein recovery, while overly strong lysis conditions can affect protein-protein interactions. This balanced RIPA lysis buffer provides reliable whole-cell lysate preparation for downstream Western blot, SDS-PAGE, immunoprecipitation (IP), and co-immunoprecipitation (Co-IP) workflows.\u003c\/p\u003e\n\u003cp\u003eCompared with strong RIPA formulations, RIPA Lysis Buffer-middle provides moderate cell lysis capability suitable for most commonly used biological samples, including cultured mammalian cells and tissues. The resulting protein lysates are suitable for total protein extraction, protein expression analysis, and biochemical research applications requiring consistent sample quality.\u003c\/p\u003e\n\u003cp\u003eRIPA Lysis Buffer-middle is part of a RIPA lysis buffer series with different extraction strengths, allowing researchers to select an appropriate protein extraction buffer according to sample characteristics, target protein solubility, and experimental requirements.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nSPECIFICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eRIPA Lysis Buffer-middle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eAP0241\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eVolume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eMedium Strength RIPA Protein Extraction Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eProtein extraction, Western Blot, SDS-PAGE, Immunoprecipitation, Co-IP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSample Types\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eCells, tissues, bacteria, yeast\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eLysis Strength\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eMedium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e4 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e18 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFEATURES\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBalanced protein extraction performance:\u003c\/strong\u003e Provides moderate lysis strength for efficient protein solubilization while maintaining compatibility with common protein analysis workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eReliable cell and tissue lysis:\u003c\/strong\u003e Suitable for preparing whole-cell lysates and tissue protein extracts from various biological samples.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBroad downstream compatibility:\u003c\/strong\u003e Supports Western blot, SDS-PAGE, immunoprecipitation, and co-immunoprecipitation applications.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSuitable for routine protein sample preparation:\u003c\/strong\u003e Provides a practical solution for researchers performing regular protein expression and biochemical analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eFlexible RIPA buffer selection:\u003c\/strong\u003e Available together with strong and weak RIPA formulations for different protein extraction requirements.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nAPPLICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eRIPA Lysis Buffer-middle is widely used as a medium-strength cell lysis buffer and protein extraction reagent in molecular biology, cell biology, and biochemical research workflows.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eWestern Blot Protein Analysis:\u003c\/strong\u003e Generates high-quality protein lysates suitable for SDS-PAGE separation and Western blot detection of target proteins.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eWhole Cell Protein Extraction:\u003c\/strong\u003e Enables preparation of whole-cell lysates from adherent cells and suspension cells for protein expression studies.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTissue Protein Extraction:\u003c\/strong\u003e Supports extraction of total proteins from tissue samples for biochemical characterization.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eImmunoprecipitation (IP):\u003c\/strong\u003e Provides protein lysates for antibody-based enrichment and target protein isolation workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCo-Immunoprecipitation (Co-IP):\u003c\/strong\u003e Suitable for studying protein-protein interactions and protein complex formation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSDS-PAGE Sample Preparation:\u003c\/strong\u003e Produces protein samples compatible with electrophoretic separation and molecular weight analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCell Signaling Research:\u003c\/strong\u003e Used for analyzing cellular signaling pathways and changes in protein expression levels.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBiochemical Research:\u003c\/strong\u003e Applicable for general protein lysate preparation and laboratory protein analysis workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPROTOCOL OVERVIEW\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eProtocol Overview\u003c\/h2\u003e\n\u003cp\u003eFor optimal protein extraction performance, samples should be processed under cold conditions to minimize protein degradation.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eAdd protease inhibitors or phosphatase inhibitors when required by the experimental design.\u003c\/li\u003e\n\u003cli\u003ePerform cell or tissue lysis on ice or at 4 °C.\u003c\/li\u003e\n\u003cli\u003eAllow sufficient incubation time for complete sample disruption and protein solubilization.\u003c\/li\u003e\n\u003cli\u003eCentrifuge lysates at 10,000–14,000 ×g for 5 minutes and collect the clarified supernatant for downstream analysis.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eSince RIPA Lysis Buffer-middle contains detergents, BCA or Lowry protein assays are recommended for protein concentration determination instead of Bradford assays.\u003c\/p\u003e\n\u003ch2\u003eOrdering Information\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eAP0241\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eRIPA Lysis Buffer-middle\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFAQ\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is RIPA Lysis Buffer-middle used for?\u003c\/summary\u003e\n\u003cp\u003eRIPA Lysis Buffer-middle is a medium-strength cell lysis buffer designed for protein extraction from cells and tissues. It is commonly used for preparing protein lysates for Western blot, SDS-PAGE, immunoprecipitation, and co-immunoprecipitation experiments.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the advantage of using medium-strength RIPA buffer?\u003c\/summary\u003e\n\u003cp\u003eMedium-strength RIPA buffer provides a balance between protein solubilization efficiency and preservation of protein complexes. It is suitable for many routine protein extraction applications where strong or mild lysis conditions are not ideal.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow should I choose between strong, medium, and weak RIPA buffers?\u003c\/summary\u003e\n\u003cp\u003eThe choice depends on sample type and experimental goals. Strong RIPA buffer is generally selected for difficult-to-solubilize proteins, while medium RIPA buffer is suitable for general protein extraction. Weak RIPA buffer may be preferred when maintaining protein interactions is a priority.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan RIPA Lysis Buffer-middle be used for Western blot?\u003c\/summary\u003e\n\u003cp\u003eYes. Protein lysates prepared with RIPA Lysis Buffer-middle are compatible with SDS-PAGE and Western blot workflows for analyzing protein expression and molecular signaling pathways.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhich protein assay method is recommended after RIPA extraction?\u003c\/summary\u003e\n\u003cp\u003eBecause RIPA buffer contains detergents, Bradford protein assays may be affected. Detergent-compatible methods such as BCA or Lowry assays are recommended for accurate protein concentration measurement.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhy is a transparent gel-like material sometimes observed after RIPA lysis?\u003c\/summary\u003e\n\u003cp\u003eA transparent gel-like material may appear because of genomic DNA and DNA-protein complexes released during cell disruption. This is a common feature of RIPA lysates and can usually be removed by centrifugation before collecting the protein-containing supernatant.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPRODUCT SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!-- =========================\nFAQ SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AccuRef Scientific","offers":[{"title":"100mL","offer_id":52775887667381,"sku":"AP0241","price":61.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/5_51e82d4f-457f-4ce7-a1cb-0c72c282b42f.jpg?v=1783480493","url":"https:\/\/biofargo.com\/products\/ripa-lysis-buffer-middle","provider":"Biofargo","version":"1.0","type":"link"}