{"product_id":"ripa-lysis-buffer-low","title":"RIPA Lysis Buffer (low)","description":"\u003c!-- =========================\nPRODUCT DESCRIPTION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eRIPA Lysis Buffer-low is a mild RIPA protein extraction buffer designed for gentle cell and tissue lysis while maintaining protein integrity and native protein interactions. During protein sample preparation, overly harsh extraction conditions may disrupt protein complexes or affect protein activity, which can impact downstream immunoprecipitation and protein interaction studies. This mild RIPA lysis buffer provides efficient cell disruption with reduced extraction strength, helping researchers obtain reliable whole-cell lysates for Western blot, SDS-PAGE, immunoprecipitation (IP), and co-immunoprecipitation (Co-IP) workflows.\u003c\/p\u003e\n\u003cp\u003eCompared with stronger RIPA formulations, RIPA Lysis Buffer-low provides a gentler extraction environment that is suitable for routine protein extraction, protein expression analysis, and experiments requiring preservation of protein complexes. The resulting protein lysates are compatible with common molecular biology and biochemical applications, including whole-cell protein extraction, native protein analysis, and antibody-based enrichment workflows.\u003c\/p\u003e\n\u003cp\u003eRIPA Lysis Buffer-low is part of a RIPA lysis buffer series with different extraction strengths, allowing researchers to select an appropriate protein extraction buffer according to sample type, target protein characteristics, and experimental requirements.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nSPECIFICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eRIPA Lysis Buffer-low\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eAP0251\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eVolume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eMild RIPA Protein Extraction Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eProtein extraction, Western Blot, SDS-PAGE, Immunoprecipitation, Co-IP, ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSample Types\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eCells, tissues, bacteria, yeast\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e4 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e18 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFEATURES\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eGentle protein extraction:\u003c\/strong\u003e Provides mild cell lysis conditions for obtaining soluble protein lysates while helping maintain protein integrity.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSuitable for protein interaction studies:\u003c\/strong\u003e The mild extraction environment is suitable for workflows where preservation of protein complexes and native interactions is important.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eIntegrated protease inhibition system:\u003c\/strong\u003e Contains multiple protease inhibitors to help reduce protein degradation during sample preparation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBroad sample compatibility:\u003c\/strong\u003e Suitable for protein extraction from adherent cells, suspension cells, tissues, bacteria, and yeast samples.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eFlexible RIPA buffer selection:\u003c\/strong\u003e Available as part of a RIPA lysis buffer series with low, medium, and high extraction strengths.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nAPPLICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eRIPA Lysis Buffer-low is widely used as a mild protein extraction reagent for molecular biology, cell biology, and biochemical research workflows where gentle cell lysis and protein preservation are required.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eWestern Blot Protein Extraction:\u003c\/strong\u003e Preparation of whole-cell lysates for SDS-PAGE separation and Western blot detection of target proteins.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eImmunoprecipitation (IP):\u003c\/strong\u003e Provides a suitable immunoprecipitation buffer environment for antibody-based protein enrichment workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCo-Immunoprecipitation (Co-IP):\u003c\/strong\u003e Supports protein-protein interaction studies by helping maintain protein complexes during extraction.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eWhole Cell Protein Extraction:\u003c\/strong\u003e Used for routine extraction of cellular proteins from adherent and suspension cultured cells.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTissue Protein Extraction:\u003c\/strong\u003e Suitable for extracting proteins from biological tissues when moderate extraction strength is preferred.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSDS-PAGE Sample Preparation:\u003c\/strong\u003e Provides protein lysates compatible with electrophoretic separation and downstream analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eELISA Sample Preparation:\u003c\/strong\u003e Supports preparation of protein samples for immunoassay-based research applications.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein Interaction Research:\u003c\/strong\u003e Used in biochemical studies requiring preservation of native protein associations.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPROTOCOL OVERVIEW\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eProtocol Overview\u003c\/h2\u003e\n\u003cp\u003eFor optimal protein extraction performance, samples should be processed under cold conditions to minimize protein degradation.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eAdd appropriate protease inhibitors or phosphatase inhibitors according to experimental requirements before use.\u003c\/li\u003e\n\u003cli\u003ePerform cell or tissue lysis on ice or at 4 °C to maintain protein stability.\u003c\/li\u003e\n\u003cli\u003eAllow sufficient incubation time for complete sample disruption while avoiding unnecessary over-lysis.\u003c\/li\u003e\n\u003cli\u003eCentrifuge lysates at 10,000–14,000 ×g for 3–5 minutes and collect the clarified supernatant for downstream analysis.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eBecause RIPA buffer contains detergents, protein concentration should be measured using detergent-compatible methods such as BCA or Lowry assays instead of Bradford assays.\u003c\/p\u003e\n\u003ch2\u003eOrdering Information\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eAP0251\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eRIPA Lysis Buffer-low\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFAQ\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is RIPA Lysis Buffer-low used for?\u003c\/summary\u003e\n\u003cp\u003eRIPA Lysis Buffer-low is a mild RIPA protein extraction buffer used for gentle cell and tissue lysis. It is designed for preparing protein lysates for Western blot, SDS-PAGE, immunoprecipitation, Co-IP, and other protein analysis workflows.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the difference between mild and strong RIPA buffer?\u003c\/summary\u003e\n\u003cp\u003eMild RIPA buffer provides gentler extraction conditions that are suitable when maintaining protein integrity or protein complexes is important. Strong RIPA buffer provides higher disruption capability for difficult-to-solubilize proteins and stronger cellular structures.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan RIPA Lysis Buffer-low be used for Western blot?\u003c\/summary\u003e\n\u003cp\u003eYes. RIPA Lysis Buffer-low can be used for Western blot protein extraction by preparing whole-cell lysates or tissue protein extracts before SDS-PAGE and antibody-based detection.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs mild RIPA buffer suitable for immunoprecipitation and Co-IP?\u003c\/summary\u003e\n\u003cp\u003eYes. Mild RIPA lysis conditions are commonly selected for immunoprecipitation and co-immunoprecipitation workflows because they help preserve protein-protein interactions compared with stronger extraction conditions.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhich samples can be extracted using RIPA Lysis Buffer-low?\u003c\/summary\u003e\n\u003cp\u003eRIPA Lysis Buffer-low can be used for protein extraction from adherent cells, suspension cells, tissue samples, bacteria, and yeast.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhy should BCA or Lowry assays be used instead of Bradford after RIPA extraction?\u003c\/summary\u003e\n\u003cp\u003eRIPA buffer contains detergents that may interfere with Bradford protein assays. BCA or Lowry protein assays are recommended because they provide better compatibility with detergent-containing protein lysates.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhy does RIPA lysate sometimes contain a transparent gel-like material?\u003c\/summary\u003e\n\u003cp\u003eA transparent gel-like material may appear after RIPA lysis due to genomic DNA and DNA-protein complexes. This is a common occurrence and usually does not affect downstream protein analysis after centrifugation and collection of the clarified lysate.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPRODUCT SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!-- =========================\nFAQ SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AccuRef Scientific","offers":[{"title":"100mL","offer_id":52775907393717,"sku":"AP0251","price":61.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/5_e69bf41e-aec0-44f2-89a8-ead8090bf846.jpg?v=1783480983","url":"https:\/\/biofargo.com\/products\/ripa-lysis-buffer-low","provider":"Biofargo","version":"1.0","type":"link"}